Functional characterization of a supramolecular affinity switch at the single molecule level

Nanoscale ◽  
2011 ◽  
Vol 3 (11) ◽  
pp. 4859 ◽  
Author(s):  
Volker Walhorn ◽  
Christian Schäfer ◽  
Tobias Schröder ◽  
Jochen Mattay ◽  
Dario Anselmetti
Keyword(s):  
Single Molecule ◽  
Functional Characterization ◽  
Single Molecule Level

scholarly journals Characterization of Fluorescent Base Analogs to Study DNA Base Flipping at the Single Molecule Level

2013 ◽  
Vol 104 (2) ◽  
pp. 346a
Author(s):  
Xochitl A. Sosa-Vazquez ◽  
Matthew Vander-Schuur ◽  
Liza Valencia ◽  
Elvin A. Aleman
Keyword(s):  
Single Molecule ◽  
Base Flipping ◽  
Single Molecule Level ◽  
Dna Base

Characterization of Pteridines: a New Approach by Fluorescence Correlation Spectroscopy and Analysis of Assay Sensitivity

Pteridines ◽  
2001 ◽  
Vol 12 (4) ◽  
pp. 147-153 ◽  
Author(s):  
U. Demel ◽  
Z. Foldes-Papp ◽  
D. Fuchs ◽  
G. P. Tilz
Keyword(s):  
Single Molecule ◽  
Fluorescence Correlation Spectroscopy ◽  
Correlation Spectroscopy ◽  
Cell Mediated Immunity ◽  
Distribution Analysis ◽  
Assay Sensitivity ◽  
New Approach ◽  
Single Molecule Level ◽  
Fluorescence Correlation

Abstract In the present investigation, fluorescence con-elation spectroscopy (FCS) was used to measure the molecular motion of the pteridine derivative neopterin. However, technical limitations in the present optical setup precluded the identification of ,single neopterin molecules. FCS measurements with a fluorophore were also can-ied out for comparison. Exemplified by rhodamine green, we have introduced a concept that allows the detection, identification and analysis of assays in solution at the single-molecule level in tenns of bulk concentration. This concept is based on FCS and Poisson distribution analysis of assay sensitivity. The molecules had not to be quantified in a more concentrated fonn, or in flow and trapping experiments. The study demonstrated an ultrasensitive, reliable, rapid and direct tool for analytics and diagnostics in solution. We discuss a possible application of our new concept in activation control of cell-mediated immunity via neopterin determination.

scholarly journals Kinetic Characterization of Nonmuscle Myosin IIB at the Single Molecule Level

2013 ◽  
Vol 104 (2) ◽  
pp. 642a
Author(s):  
Attila Nagy ◽  
Yasuharu Takagi ◽  
Neil Billington ◽  
Earl Homsher ◽  
James R. Sellers
Keyword(s):  
Single Molecule ◽  
Kinetic Characterization ◽  
Nonmuscle Myosin ◽  
Single Molecule Level

scholarly journals From Starphenes to Non-Benzenoid Linear Conjugated Polymers by Substrate Templating

2020 ◽  
Author(s):  
Mohammed S. G. Mohammed ◽  
James Lawrence ◽  
Fátima García ◽  
Pedro Brandimarte ◽  
Alejandro Berdonces-Layunta ◽  
...  
Keyword(s):  
Conjugated Polymers ◽  
Single Molecule ◽  
Molecular Structures ◽  
Synthetic Methods ◽  
Synthesis And Characterization ◽  
Scanning Tunneling ◽  
Tunneling Microscopy ◽  
Single Molecule Level ◽  
Templating Effect

Combining on-surface synthetic methods with the power of scanning tunneling microscopy to characterize novel materials at the single molecule level, we show how to steer the reactivity of one anthracene-based precursor towards different product nanostructures. Whereas using a two-dimensional Au(111) surface results in the dominant formation of a starphene derivative, the templating effect of a reconstructed Au(110) surface allows the selective growth of non-benzenoid linear conjugated polymers. We further assess the electronic properties of each of the observed product structures via tunneling spectroscopy and DFT calculations, altogether advancing in the synthesis and characterization of molecular structures of notable scientific interest that have been only scarcely investigated to date, as applied to both starphenes and to non-benzenoid conjugated polymers. <br>

scholarly journals Fluorescence-free quantification of protein/nucleic-acid binding through single-molecule kinetic locking

2020 ◽  
Author(s):  
Martin Rieu ◽  
Jessica Valle-Orero ◽  
Bertrand Ducos ◽  
Jean-François Allemand ◽  
Vincent Croquette
Keyword(s):  
Single Molecule ◽  
Functional Characterization ◽  
Nucleic Acid Binding ◽  
Single Molecule Force Spectroscopy ◽  
E Coli ◽  
Natural Function ◽  
Protein Nucleic Acid ◽  
Free Quantification

ABSTRACTFluorescence-free micro-manipulation of nucleic acids (NA) allows the functional characterization of DNA/RNA processing proteins, without the interference of labels, but currently fails to detect and quantify their binding. To overcome this limitation, we developed a new method based on single-molecule force spectroscopy, called kinetic locking, that allows a direct in vitro visualization of protein binding while avoiding any kind of chemical disturbance of the protein’s natural function. We validate kinetic locking by measuring accurately the hybridization energy of ultrashort nucleotides (5,6,7 bases) and use it to measure the dynamical interactions of E. coli RecQ helicase with its DNA substrate.

scholarly journals Kinetic Characterization of Non-Muscle Myosin IIB Single-Headed Heavy Meromyosin on Single Molecule Level with Optical Tweezers

2010 ◽  
Vol 98 (3) ◽  
pp. 561a
Author(s):  
Attila Nagy ◽  
Yasuharu Takagi ◽  
Earl E. Homsher ◽  
Davin K.T. Hong ◽  
Mihaly Kovacs ◽  
...  
Keyword(s):  
Optical Tweezers ◽  
Single Molecule ◽  
Kinetic Characterization ◽  
Heavy Meromyosin ◽  
Single Molecule Level ◽  
Muscle Myosin

Synthesis and Characterization of Long Perylenediimide Polymer Fibers:  From Bulk to the Single-Molecule Level

2006 ◽  
Vol 110 (15) ◽  
pp. 7803-7812 ◽  
Author(s):  
Pieter A. J. De Witte ◽  
Jordi Hernando ◽  
Edda E. Neuteboom ◽  
Erik M. H. P. van Dijk ◽  
Stefan C. J. Meskers ◽  
...  
Keyword(s):  
Single Molecule ◽  
Polymer Fibers ◽  
Synthesis And Characterization ◽  
Single Molecule Level

Spectroscopic characterization of Venus at the single molecule level

2012 ◽  
Vol 11 (2) ◽  
pp. 358 ◽  
Author(s):  
Charlotte C. David ◽  
Peter Dedecker ◽  
Gert De Cremer ◽  
Natalie Verstraeten ◽  
Cyrielle Kint ◽  
...  
Keyword(s):  
Single Molecule ◽  
Spectroscopic Characterization ◽  
Single Molecule Level

scholarly journals Single-molecule kinetic locking allows fluorescence-free quantification of protein/nucleic-acid binding

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Martin Rieu ◽  
Jessica Valle-Orero ◽  
Bertrand Ducos ◽  
Jean-François Allemand ◽  
Vincent Croquette
Keyword(s):  
Single Molecule ◽  
Functional Characterization ◽  
Nucleic Acid Binding ◽  
Single Molecule Force Spectroscopy ◽  
E Coli ◽  
Natural Function ◽  
Protein Nucleic Acid ◽  
Free Quantification

AbstractFluorescence-free micro-manipulation of nucleic acids (NA) allows the functional characterization of DNA/RNA processing proteins, without the interference of labels, but currently fails to detect and quantify their binding. To overcome this limitation, we developed a method based on single-molecule force spectroscopy, called kinetic locking, that allows a direct in vitro visualization of protein binding while avoiding any kind of chemical disturbance of the protein’s natural function. We validate kinetic locking by measuring accurately the hybridization energy of ultrashort nucleotides (5, 6, 7 bases) and use it to measure the dynamical interactions of Escherichia coli/E. coli RecQ helicase with its DNA substrate.

scholarly journals From Starphenes to Non-Benzenoid Linear Conjugated Polymers by Substrate Templating

2020 ◽  
Author(s):  
Mohammed S. G. Mohammed ◽  
James Lawrence ◽  
Fátima García ◽  
Pedro Brandimarte ◽  
Alejandro Berdonces-Layunta ◽  
...  
Keyword(s):  
Conjugated Polymers ◽  
Single Molecule ◽  
Molecular Structures ◽  
Synthetic Methods ◽  
Synthesis And Characterization ◽  
Scanning Tunneling ◽  
Tunneling Microscopy ◽  
Single Molecule Level ◽  
Templating Effect

Combining on-surface synthetic methods with the power of scanning tunneling microscopy to characterize novel materials at the single molecule level, we show how to steer the reactivity of one anthracene-based precursor towards different product nanostructures. Whereas using a two-dimensional Au(111) surface results in the dominant formation of a starphene derivative, the templating effect of a reconstructed Au(110) surface allows the selective growth of non-benzenoid linear conjugated polymers. We further assess the electronic properties of each of the observed product structures via tunneling spectroscopy and DFT calculations, altogether advancing in the synthesis and characterization of molecular structures of notable scientific interest that have been only scarcely investigated to date, as applied to both starphenes and to non-benzenoid conjugated polymers. <br>

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