Dark-field microscopy studies of polarization-dependent plasmonic resonance of single gold nanorods: rainbow nanoparticles

Nanoscale ◽  
2011 ◽  
Vol 3 (8) ◽  
pp. 3228 ◽  
Author(s):  
Youju Huang ◽  
Dong-Hwan Kim
2006 ◽  
Author(s):  
Vladimir A. Bogatyrev ◽  
Lev A. Dykman ◽  
Anna V. Alekseeva ◽  
Boris N. Khlebtsov ◽  
Anna P. Novikova ◽  
...  

Nanophotonics ◽  
2017 ◽  
Vol 7 (2) ◽  
pp. 497-505 ◽  
Author(s):  
Pedram Sadeghi ◽  
Kaiyu Wu ◽  
Tomas Rindzevicius ◽  
Anja Boisen ◽  
Silvan Schmid

AbstractWe report on the fabrication and dark-field spectroscopy characterization of Au dimer nanoantennas placed on top of SiO2 nanopillars. The reported process enables the fabrication of nanopillar dimers with gaps down to 15 nm and heights up to 1 μm. A clear dependence of the plasmonic resonance position on the dimer gap is observed for smaller pillar heights, showing the high uniformity and reproducibility of the process. It is shown how increasing the height of nanopillars significantly affects the recorded elastic scattering spectra from Au nanoantennas. The results are compared to finite-difference time-domain (FDTD) and finite-element method (FEM) simulations. Additionally, measured spectra are accompanied by dark-field microscopy images of the dimers, showing the pronounced change in color. Placing nanoantennas on nanopillars with a height comparable to the in-plane dimer dimensions results in an enhancement of the scattering response, which can be understood through reduced interaction of the near-fields with the substrate. When increasing the pillar height further, scattering by the pillars themselves manifests itself as a strong tail at lower wavelengths. Additionally, strong directional scattering is expected as a result of the interface between the nanoantennas and nanopillars, which is taken into account in simulations. For pillars of height close to the plasmonic resonance wavelength, the scattering spectra become more complex due to additional scattering peaks as a result of larger geometrical nonuniformities.


The Analyst ◽  
2018 ◽  
Vol 143 (4) ◽  
pp. 824-828 ◽  
Author(s):  
Jian Wang ◽  
Hong Zhi Zhang ◽  
Jia Jun Liu ◽  
Dan Yuan ◽  
Rong Sheng Li ◽  
...  

Plasmonic gold nanorods are promising and sensitive light scattering probes, which can reach the single particle level. Herein, we present the scattering properties of gold nanorods for time-resolved visual detection of heparin based on the rapid etching of gold nanorods under dark-field microscopy.


Author(s):  
J. Langmore ◽  
M. Isaacson ◽  
J. Wall ◽  
A. V. Crewe

High resolution dark field microscopy is becoming an important tool for the investigation of unstained and specifically stained biological molecules. Of primary consideration to the microscopist is the interpretation of image Intensities and the effects of radiation damage to the specimen. Ignoring inelastic scattering, the image intensity is directly related to the collected elastic scattering cross section, σɳ, which is the product of the total elastic cross section, σ and the eficiency of the microscope system at imaging these electrons, η. The number of potentially bond damaging events resulting from the beam exposure required to reduce the effect of quantum noise in the image to a given level is proportional to 1/η. We wish to compare η in three dark field systems.


Author(s):  
William Krakow

Tilted beam dark-field microscopy has been applied to atomic structure determination in perfect crystals, several synthesized molecules with heavy atcm markers and in the study of displaced atoms in crystals. Interpretation of this information in terms of atom positions and atom correlations is not straightforward. Therefore, calculated dark-field images can be an invaluable aid in image interpretation.


Cancers ◽  
2021 ◽  
Vol 13 (14) ◽  
pp. 3509
Author(s):  
Paule Marcoux-Valiquette ◽  
Cécile Darviot ◽  
Lu Wang ◽  
Andrée-Anne Grosset ◽  
Morteza Hasanzadeh Kafshgari ◽  
...  

Reliable cytopathological diagnosis requires new methods and approaches for the rapid and accurate determination of all cell types. This is especially important when the number of cells is limited, such as in the cytological samples of fine-needle biopsy. Immunoplasmonic-multiplexed- labeling may be one of the emerging solutions to such problems. However, to be accepted and used by the practicing pathologists, new methods must be compatible and complementary with existing cytopathology approaches where counterstaining is central to the correct interpretation of immunolabeling. In addition, the optical detection and imaging setup for immunoplasmonic-multiplexed-labeling must be implemented on the same cytopathological microscope, not interfere with standard H&E imaging, and operate as a second easy-to-use imaging method. In this article, we present multiplex imaging of four types of nanoplasmonic markers on two types of H&E-stained cytological specimens (formalin-fixed paraffin embedded and non-embedded adherent cancer cells) using a specially designed adapter for SI dark-field microscopy. The obtained results confirm the effectiveness of the proposed optical method for quantitative and multiplex identification of various plasmonic NPs, and the possibility of using immunoplasmonic-multiplexed-labeling for cytopathological diagnostics.


Nanoscale ◽  
2021 ◽  
Author(s):  
Paula Zamora-Perez ◽  
Beatriz Pelaz ◽  
Dionysia Tsoutsi ◽  
Mahmoud G. Soliman ◽  
Wolfgang J. Parak ◽  
...  

Hyperspectral-enhanced dark field microscopy to correlate Au/CuS NPs’ changes in their physicochemical properties induced by cellular environments with their functionality as photothermal probes by tracking their scattering profile evolution in real time.


2020 ◽  
Vol 64 (3) ◽  
Author(s):  
Eva Jennische ◽  
Stefan Lange ◽  
Ragnar Hultborn

A simple dark field microscopy technique was used for visualization of blood vessels in normal human renal tissues and carcinoma. Phase contrast condenser ring apt for high power objectives was combined with a 10x objective in order to create a dark field illumination of the specimens examined. The endothelial lining of the vessels had been stained by using CD31 monoclonal antibodies combined with conventional peroxidase immunohistochemistry. The final DAB addition used for this technique induced an intense light scatter in the dark field microscope. This scattered light originating from the endothelial lining made the walls of the bright vessels easily detectable from the dark background.


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