In situ monitoring of single molecule binding reactions with time-lapse atomic force microscopy on functionalized DNA origami

Nanoscale ◽  
2011 ◽  
Vol 3 (6) ◽  
pp. 2481 ◽  
Author(s):  
Na Wu ◽  
Xingfei Zhou ◽  
Daniel M. Czajkowsky ◽  
Ming Ye ◽  
Dongdong Zeng ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Single Molecule ◽  
Time Lapse ◽  
Dna Origami ◽  
In Situ Monitoring ◽  
Force Microscopy ◽  
Atomic Force

scholarly journals Magnesium-Free Immobilization of DNA Origami Nanostructures at Mica Surfaces for Atomic Force Microscopy

Molecules ◽  
2021 ◽  
Vol 26 (16) ◽  
pp. 4798
Author(s):  
Yang Xin ◽  
Amir Ardalan Zargariantabrizi ◽  
Guido Grundmeier ◽  
Adrian Keller
Keyword(s):  
Atomic Force Microscopy ◽  
Single Molecule ◽  
Pure Water ◽  
Dna Origami ◽  
Force Microscopy ◽  
Atomic Force ◽  
The Individual ◽  
Individual Strategies ◽  
Phosphate Buffered Saline

DNA origami nanostructures (DONs) are promising substrates for the single-molecule investigation of biomolecular reactions and dynamics by in situ atomic force microscopy (AFM). For this, they are typically immobilized on mica substrates by adding millimolar concentrations of Mg2+ ions to the sample solution, which enable the adsorption of the negatively charged DONs at the like-charged mica surface. These non-physiological Mg2+ concentrations, however, present a serious limitation in such experiments as they may interfere with the reactions and processes under investigation. Therefore, we here evaluate three approaches to efficiently immobilize DONs at mica surfaces under essentially Mg2+-free conditions. These approaches rely on the pre-adsorption of different multivalent cations, i.e., Ni2+, poly-l-lysine (PLL), and spermidine (Spdn). DON adsorption is studied in phosphate-buffered saline (PBS) and pure water. In general, Ni2+ shows the worst performance with heavily deformed DONs. For 2D DON triangles, adsorption at PLL- and in particular Spdn-modified mica may outperform even Mg2+-mediated adsorption in terms of surface coverage, depending on the employed solution. For 3D six-helix bundles, less pronounced differences between the individual strategies are observed. Our results provide some general guidance for the immobilization of DONs at mica surfaces under Mg2+-free conditions and may aid future in situ AFM studies.

scholarly journals Nanomechanical DNA origami 'single-molecule beacons' directly imaged by atomic force microscopy

2011 ◽  
Vol 2 (1) ◽  
Author(s):  
Akinori Kuzuya ◽  
Yusuke Sakai ◽  
Takahiro Yamazaki ◽  
Yan Xu ◽  
Makoto Komiyama
Keyword(s):  
Atomic Force Microscopy ◽  
Single Molecule ◽  
Dna Origami ◽  
Force Microscopy ◽  
Atomic Force

In situ single molecule detection of insulin receptors on erythrocytes from a type 1 diabetes ketoacidosis patient by atomic force microscopy

The Analyst ◽  
2015 ◽  
Vol 140 (21) ◽  
pp. 7407-7416 ◽  
Author(s):  
Lu Zhang ◽  
Jiang Pi ◽  
Qiping Shi ◽  
Jiye Cai ◽  
Peihui Yang ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Type 1 Diabetes ◽  
Single Molecule ◽  
Insulin Receptors ◽  
Single Molecule Detection ◽  
Force Microscopy ◽  
Atomic Force ◽  
Molecule Interactions

A method to investigate the single molecule interactions between insulin and insulin receptor in erythrocytes from healthy volunteer and type 1 diabetes ketoacidosis (T1-DKA) patient was introduced using atomic force microscopy (AFM).

Single Molecule Atomic Force Microscopy Studies of Photosensitized Singlet Oxygen Behavior on a DNA Origami Template

ACS Nano ◽  
2010 ◽  
Vol 4 (12) ◽  
pp. 7475-7480 ◽  
Author(s):  
Sarah Helmig ◽  
Alexandru Rotaru ◽  
Dumitru Arian ◽  
Larisa Kovbasyuk ◽  
Jacob Arnbjerg ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Singlet Oxygen ◽  
Single Molecule ◽  
Dna Origami ◽  
Force Microscopy ◽  
Atomic Force

scholarly journals Tuning and in situ monitoring of surface-initiated, atom-transfer radical polymerization of acrylamide derivatives in water-based solvents

2019 ◽  
Vol 10 (29) ◽  
pp. 3933-3942 ◽  
Author(s):  
Joydeb Mandal ◽  
Rok Simic ◽  
Nicholas D. Spencer
Keyword(s):  
Atomic Force Microscopy ◽  
Quartz Crystal ◽  
Polymer Brush ◽  
In Situ Monitoring ◽  
Colloidal Probe ◽  
Force Microscopy ◽  
Atomic Force ◽  
Water Based ◽  
Kinetics Of

SI-ATRP kinetics of acrylamide derivatives is studied in situ using a quartz crystal microbalance with dissipation (QCM-D). The effect of growth kinetics on polymer-brush dispersity have been examined using colloidal-probe atomic force microscopy.

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