Cellular uptake mechanisms of functionalised multi-walled carbon nanotubes by 3D electron tomography imaging

Nanoscale ◽  
2011 ◽  
Vol 3 (6) ◽  
pp. 2627 ◽  
Author(s):  
Khuloud T. Al-Jamal ◽  
Hannah Nerl ◽  
Karin H. Müller ◽  
Hanene Ali-Boucetta ◽  
Shouping Li ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Cellular Uptake ◽  
Electron Tomography ◽  
Tomography Imaging ◽  
Multi Walled Carbon Nanotubes ◽  
3D Electron ◽  
Walled Carbon Nanotubes ◽  
Uptake Mechanisms

A comparative study of cellular uptake and cytotoxicity of multi-walled carbon nanotubes, graphene oxide, and nanodiamond

2012 ◽  
Vol 1 (1) ◽  
pp. 62-68 ◽  
Author(s):  
Xiaoyong Zhang ◽  
Wenbing Hu ◽  
Jing Li ◽  
Lei Tao ◽  
Yen Wei
Keyword(s):  
Carbon Nanotubes ◽  
Graphene Oxide ◽  
Cellular Uptake ◽  
Hela Cells ◽  
Carbon Nanomaterials ◽  
Toxicity Assessment ◽  
Cell Uptake ◽  
Multi Walled Carbon Nanotubes ◽  
Living Organisms ◽  
Walled Carbon Nanotubes

Abstract Investigations of the interactions between carbon nanomaterials (CNMs) and living organisms and their subsequent biological responses are of fundamental significance for toxicity assessment and further biomedical applications. In this work, the cellular uptake and cytotoxicity of multi-walled carbon nanotubes (MWCNTs), graphene oxide (GO) and nanodiamond (ND) were examined and compared. We demonstrated that all of the CNMs were readily internalized by HeLa cells through nonspecific cellular uptake. Their cell uptake ratios showed significant differences in the following order: ND > MWCNTs > GO. A series of biological assays were used to evaluate the cytotoxicity of CNMs. It was found that CNMs showed dose- and time-dependent cytotoxicity to HeLa cells. However, cytotoxicity of CNMs was not associated with their cell uptake ratios. Among them, ND exhibited the highest cell uptake ratio and the least cytotoxicity. To the best of our knowledge, this is the first study which has quantitatively determined and compared the cell uptake ratios and cytotoxicities of MWCNTs, GO and ND. And we expect that these results described here could provide useful information for the development of new strategies to design efficient drug delivery nanocarriers and therapeutic systems as well as deep insights into the mechanism of CNMs' cytotoxicity.

Multi-walled Carbon Nanotubes Penetrate into Plant Cells and Affect the Growth of Onobrychis arenaria Seedlings

Acta Naturae ◽  
2011 ◽  
Vol 3 (1) ◽  
pp. 99-106 ◽  
Author(s):  
E A Smirnova ◽  
A A Gusev ◽  
O N Zaitseva ◽  
E M Lazareva ◽  
G E Onishchenko ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Plant Cells ◽  
Multi Walled Carbon Nanotubes ◽  
Walled Carbon Nanotubes

Incorporation of Multi Wall Carbon Nanotubes into Glass-Surfaces via Laser-Treatment

2003 ◽  
Vol 772 ◽  
Author(s):  
T. Seeger ◽  
G. de la Fuente ◽  
W.K. Maser ◽  
A.M. Benito ◽  
A. Righi ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Continuous Wave ◽  
Silica Surface ◽  
Multi Wall Carbon Nanotubes ◽  
Composite Formation ◽  
Multi Walled Carbon Nanotubes ◽  
Glass Surfaces ◽  
Walled Carbon Nanotubes ◽  
First Time ◽  
Scanning Electron

AbstractCarbon nanotubes (CNT) are interesting candidates for the reinforcement in robust composites and for conducting fillers in polymers due to their fascinating electronic and mechanical properties. For the first time, we report the incorporation of multi walled carbon nanotubes (MWNTs) into silica-glass surfaces by means of partial surface-melting caused by a continuous wave Nd:YAG laser. MWNTs were detected being well incorporated in the silica-surface. The composites are characterized using scanning electron microscopy (SEM) and Raman-spectroscopy. A model for the composite-formation is proposed based on heatabsorption by MWNTs and a partial melting of the silica-surface.

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