Metabolic pathway visualization in living yeast by DNP-NMR

2011 ◽  
Vol 7 (10) ◽  
pp. 2834 ◽  
Author(s):  
Sebastian Meier ◽  
Magnus Karlsson ◽  
Pernille R. Jensen ◽  
Mathilde H. Lerche ◽  
Jens Ø. Duus
Keyword(s):  
Metabolic Pathway ◽  
Pathway Visualization

PERLAKUAN ETHYL METHANE SULFONATE (EMS) PADA ENTEROBACTER AEROGENES AY-2 DARI LIMBAH METAN FERMENTASI UNTUK PENINGKATAN PRODUKSI GAS HIDROGEN

2018 ◽  
Vol 5 (1) ◽  
Author(s):  
Mahyudin Abdul Rachman
Keyword(s):  
Metabolic Pathway ◽  
Acid Production ◽  
Double Mutant ◽  
Enterobacter Aerogenes ◽  
H2 Production ◽  
Hydrogen Gas ◽  
Survival Ratio ◽  
Ethyl Methane Sulfonate ◽  
Methane Sulfonate ◽  
Ethyl Methane

Enterobacter aerogenes AY-2 mutant is known for hydrogen gas producer which ws obtained from the sludge of methane fermentation and the yield is 1.5 fold higher than wildtype. Hydrogen gas production can be gain via NADH oxidation in anaerobic metabolic pathway by blocking organic acid production. Metabolic pathway can be changed by mutagenesis. Enterobacter aerogenes AY-2 mutated with ethyl methane sulfonate in logarithmic phase with consentration 10, 11, 12, 13, 14 and 15 μl/ml cell suspention during 120 minute. Mutation that result lowest survival ratio (0,01%) was 14 μl EMS/ml cell suspention is repeated with variation incubation time, 30, 60, 90 and 120 minute. 166 double mutant colony has been collected and choosen randomly. The choosen 43 colony was fermented in glycerol complex medium for determining ten double mutant with the highest H2 production. Double mutant AD-H43 is a highest H2 producer that increase 20% H2 production from AY-2 and has a decrease lactid acid production, 31% less from AY-2. Increasing H2 production in double mutant AD-H43 is caused by lactate dehydrogenase deffi cient.Keywords: Enterobacter aerogenes AY-2, ethyl methane sulfonate (EMS), H2 and methane sludge

Accelerated Phenol Removal by Amplifying the Metabolic Pathway with a Recombinant Plasmid Encoding Catechol 2, 3 Oxygenase

1992 ◽  
Vol 26 (9-11) ◽  
pp. 2191-2194 ◽  
Author(s):  
M. Fujita ◽  
M. Ike ◽  
T. Kamiya
Keyword(s):  
Metabolic Pathway ◽  
Recombinant Plasmid ◽  
Removal Rate ◽  
Phenol Degradation ◽  
Wild Strain ◽  
Phenol Concentration ◽  
Phenol Removal

The metabolic pathway of the phenol degradation in Pseudomonasputida BH was amplified by introducing the recombinant plasmid containing catechol 2,3 oxygenase gene isolated fron the chromosome of BH. This strain could degrade phenol and grow much faster than the wild strain at the phenol concentration of 100mg/L. This strain seems to accelerate the phenol removal rate if it is applied to the treatment of wastewater containing phenol.

Sign in / Sign up

Export Citation Format

Share Document