Photophoretic assembly and migration of dielectric particles and Escherichia coli in liquids using a subwavelength diameter optical fiber

Lab on a Chip ◽  
2011 ◽  
Vol 11 (13) ◽  
pp. 2241 ◽  
Author(s):  
Hongxiang Lei ◽  
Yao Zhang ◽  
Xingmin Li ◽  
Baojun Li
Keyword(s):  
Escherichia Coli ◽  
Optical Fiber ◽  
Dielectric Particles ◽  
And Migration

scholarly journals Expression of wild-type and mutated rabbit osteopontin in Escherichia coli, and their effects on adhesion and migration of P388D1 cells

1995 ◽  
Vol 307 (1) ◽  
pp. 257-265 ◽  
Author(s):  
K Nasu ◽  
T Ishida ◽  
M Setoguchi ◽  
Y Higuchi ◽  
S Akizuki ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Fluid Phase ◽  
Intradermal Injection ◽  
Polymorphonuclear Leucocyte ◽  
Alpha Subunit ◽  
Wild Type ◽  
Alpha Subunits ◽  
Leucocyte Migration ◽  
And Migration ◽  
Chemotactic Effect

Recombinant wild-type rabbit osteopontin (rOP) and the protein with an aspartate-to-glutamate transposition induced by a point mutation in the rabbit OP cDNA within the Gly-Arg-Gly-Asp-Ser (GRGDS) sequence were expressed in Escherichia coli and purified to homogeneity. P388D1 cells bound rOP in a saturable manner. rOP induced adhesion and haptotaxis of P388D1 cells, whereas mutated rabbit OP (rOPmut) did not. Anti-rOP IgG F(ab′)2 and synthetic GRGDS peptide inhibited rOP-mediated adhesion and haptotaxis of P388D1 cells. Fibronectin (FN)-mediated adhesion of P388D1 cells was markedly inhibited in the presence of fluid-phase rOP. Adhesion of P388D1 cells to rOP was significantly inhibited by anti-[alpha-subunits of VLA4 (alpha 4) and VLA5 (alpha 5)] monoclonal antibodies (mAbs), but not by anti-[alpha-subunit of vitronectin (VN) receptor (alpha V) or Mac-1 (alpha M)] mAb. Adhesion of P388D1 cells to FN and VN was significantly inhibited by anti-alpha V mAb but not anti-alpha 4, -alpha 5 or -alpha M mAb. Haptotaxis of P388D1 cells to rOP was significantly inhibited by anti-alpha V mAb, but not by anti-alpha 4, -alpha 5 and alpha M mAbs, whereas that to FN showed no inhibition with all three mAbs. Haptotaxis of P388D1 cells to VN was significantly inhibited by anti-alpha 5 and -alpha V mAbs but not by anti-alpha 4 and -alpha M mAbs. Similar features of inhibition of adhesion and haptotaxis of P388D1 cells to human OP were observed by mAbs. rOP had no chemotactic effect on P388D1 cells. Significant polymorphonuclear leucocyte migration was observed 3-12 h after intradermal injection of rOP into rabbits.

scholarly journals Massive photothermal trapping and migration of particles by a tapered optical fiber

2011 ◽  
Vol 19 (18) ◽  
pp. 17065 ◽  
Author(s):  
Hongbao Xin ◽  
Xingmin Li ◽  
Baojun Li
Keyword(s):  
Optical Fiber ◽  
Tapered Optical Fiber ◽  
And Migration

Optofluidic manipulation of Escherichia coli in a microfluidic channel using an abruptly tapered optical fiber

2013 ◽  
Vol 103 (3) ◽  
pp. 033703 ◽  
Author(s):  
Hongbao Xin ◽  
Yayi Li ◽  
Lingshan Li ◽  
Rui Xu ◽  
Baojun Li
Keyword(s):  
Escherichia Coli ◽  
Optical Fiber ◽  
Microfluidic Channel ◽  
Tapered Optical Fiber

Immobilized optical fiber microprobe for selective and sensitive Escherichia coli detection

2018 ◽  
pp. e201700162
Author(s):  
Yanpeng Li ◽  
Hui Ma ◽  
Lin Gan ◽  
Andong Gong ◽  
Haibin Zhang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Optical Fiber

High sensitive and selective Escherichia coli detection using immobilized optical fiber microprobe

2017 ◽  
Author(s):  
Yanpeng Li ◽  
Qizhen Sun ◽  
Yiyang Luo ◽  
Yue Li ◽  
Andong Gong ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Optical Fiber

scholarly journals The assembly and migration of SeqA-Gfp fusion in living cells of Escherichia coli

1999 ◽  
Vol 31 (6) ◽  
pp. 1775-1782 ◽  
Author(s):  
Toshinari Onogi ◽  
Hironori Niki ◽  
Mitsuyoshi Yamazoe ◽  
Sota Hiraga
Keyword(s):  
Escherichia Coli ◽  
Living Cells ◽  
And Migration

Detection of Escherichia coli O157:H7 in 10- and 25-Gram Ground Beef Samples with an Evanescent-Wave Biosensor with Silica and Polystyrene Waveguides

2002 ◽  
Vol 65 (4) ◽  
pp. 596-602 ◽  
Author(s):  
DANIEL R. DeMARCO ◽  
DANIEL V. LIM
Keyword(s):  
Escherichia Coli ◽  
Optical Fiber ◽  
Evanescent Wave ◽  
Ground Beef ◽  
False Positives ◽  
Evanescent Field ◽  
Escherichia Coli O157 ◽  
Fiber Probe ◽  
Fluorescent Signal ◽  
E Coli

A portable evanescent-wave fiber-optic biosensor was used to detect Escherichia coli O157:H7 in seeded 10- and 25-g ground beef samples. The biosensor works by launching light from a 635-nm laser diode into specially designed optical fiber probes, generating an evanescent field that extends approximately 1,000 nm from the fiber surface. Fluorescent molecules within the evanescent field are excited, and a portion of their emission recouples into the fiber probe. The return path emission is transported by an optical fiber to a photodiode within the biosensor that detects and quantifies the fluorescent signal. A sandwich immunoassay was performed on the fiber probes with cyanine 5 dye–labeled polyclonal anti–E. coli O157:H7 antibodies for generation of the specific fluorescent signal. Biotin-streptavidin interactions were used to attach polyclonal antiE. coli O157:H7 antibodies to the surface of the fiber probe. A centrifugation method was developed to obtain samples suitable for biosensor analysis from 10- and 25-g ground beef samples. The assay was shown to be sensitive and repeatable. One hundred percent correct identification of positive samples was demonstrated at 9.0 × 103 CFU/g for 25-g ground beef samples with silica waveguides and at 5.2 × 102 CFU/g for 10-g ground beef samples with polystyrene waveguides. The reaction was highly specific. No false positives were observed for 10-g ground beef samples not spiked with the pathogen. In addition, when samples were spiked with high concentrations of a variety of non–E. coli O157:H7 organisms, no false positives were observed. The method was rapid, with results being obtained within 25 min of sample processing.

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