Using a co-culture microsystem for cell migration under fluid shear stress

Lab on a Chip ◽  
2011 ◽  
Vol 11 (15) ◽  
pp. 2583 ◽  
Author(s):  
Chia-Hsien Yeh ◽  
Shen-Hsing Tsai ◽  
Li-Wha Wu ◽  
Yu-Cheng Lin
Keyword(s):  
Shear Stress ◽  
Cell Migration ◽  
Fluid Shear Stress ◽  
Fluid Shear

scholarly journals Fluid shear stress induces cell migration and invasion via activating autophagy in HepG2 cells

2019 ◽  
Vol 13 (1) ◽  
pp. 152-163 ◽  
Author(s):  
Zhiping Yan ◽  
Guanyue Su ◽  
Wenbo Gao ◽  
Jia He ◽  
Yang Shen ◽  
...  
Keyword(s):  
Shear Stress ◽  
Cell Migration ◽  
Hepg2 Cells ◽  
Fluid Shear Stress ◽  
Migration And Invasion ◽  
Fluid Shear ◽  
Cell Migration And Invasion

scholarly journals Fluid shear stress regulates HepG2 cell migration though time-dependent integrin signaling cascade

2017 ◽  
Vol 12 (1) ◽  
pp. 56-68 ◽  
Author(s):  
Hongchi Yu ◽  
Yang Shen ◽  
Jingsi Jin ◽  
Yingying Zhang ◽  
Tang Feng ◽  
...  
Keyword(s):  
Shear Stress ◽  
Cell Migration ◽  
Hepg2 Cell ◽  
Fluid Shear Stress ◽  
Time Dependent ◽  
Integrin Signaling ◽  
Signaling Cascade ◽  
Fluid Shear

scholarly journals Fluid-Solid Coupling Simulation of Wall Fluid Shear Stress on Cells under Gradient Fluid Flow

2021 ◽  
Vol 2021 ◽  
pp. 1-12
Author(s):  
Xiao Zhang ◽  
Yan Gao ◽  
Bo Huo
Keyword(s):  
Shear Stress ◽  
Cell Migration ◽  
Cell Surface ◽  
Fluid Shear Stress ◽  
Flow Chamber ◽  
Bottom Plate ◽  
Finite Element Models ◽  
Fluid Shear ◽  
Osteoclast Precursors ◽  
Cell Spacing

Fluid shear stress (FSS) plays a crucial role for cell migration within bone cavities filled with interstitial fluid. Whether the local wall FSS distribution on cell surface depends on the global gradient FSS of flow field should be clarified to explain our previous experimental observation. In this study, finite element models of discretely distributed or hexagonal closely packed cells adherent on the bottom plate in a modified plate flow chamber with different global FSS gradient were constructed. Fluid-solid coupling simulation of wall fluid shear stress on cells was performed, and two types of data analysis methods were used. The results showed that the profile of local FSS distribution on cell surface coincides with the angle of cell migration determined in the previous study, suggesting that RAW264.7 osteoclast precursors may sense the global FSS gradient and migrate toward the low-FSS region under a high gradient. For hexagonal closely packed cells, this profile on the surface of central cells decreased along with the increase of cell spacing, which may be caused by the higher local FSS difference along the direction of FSS gradient in the regions close to the bottom plate. This study may explain the phenomenon of the targeted migration of osteoclast precursors under gradient FSS field and further provide insights into the mechanism of mechanical stimulation-induced bone remodeling.

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