DNA-templated ensemble for label-free and real-time fluorescence turn-on detection of enzymatic/oxidative cleavage of single-stranded DNA

2011 ◽  
Vol 47 (28) ◽  
pp. 8133 ◽  
Author(s):  
Xinjian Yang ◽  
Fang Pu ◽  
Jinsong Ren ◽  
Xiaogang Qu
Keyword(s):  
Real Time ◽  
Oxidative Cleavage ◽  
Label Free ◽  
Single Stranded Dna ◽  
Turn On ◽  
Fluorescence Turn On

Label-free and real-time monitoring of trypsin activity in living cells by quantum-dot-based fluorescent sensors

2014 ◽  
Vol 6 (8) ◽  
pp. 2499-2505 ◽  
Author(s):  
Wenzhu Zhang ◽  
Ping Zhang ◽  
Shengzhou Zhang ◽  
Changqing Zhu
Keyword(s):  
Quantum Dot ◽  
Real Time ◽  
Living Cells ◽  
Fluorescent Sensors ◽  
Label Free ◽  
Trypsin Activity ◽  
Real Time Monitoring ◽  
Turn On ◽  
Fluorescence Turn On

A quantum-dot-based fluorescence turn-on sensor was used for label-free and real-time monitoring of trypsin activity.

scholarly journals How to copy and paste DNA microarrays

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Stefan D. Krämer ◽  
Johannes Wöhrle ◽  
Philipp A. Meyer ◽  
Gerald A. Urban ◽  
Günter Roth
Keyword(s):  
Real Time ◽  
Dna Microarray ◽  
Dna Microarrays ◽  
Label Free ◽  
Binding Assays ◽  
Single Stranded Dna ◽  
Whole Process ◽  
Double Stranded Dna ◽  
Cost Efficient ◽  
Copy And Paste

Abstract Analogous to a photocopier, we developed a DNA microarray copy technique and were able to copy patterned original DNA microarrays. With this process the appearance of the copied DNA microarray can also be altered compared to the original by producing copies of different resolutions. As a homage to the very first photocopy made by Chester Charlson and Otto Kornei, we performed a lookalike DNA microarray copy exactly 80 years later. Those copies were also used for label-free real-time kinetic binding assays of apo-dCas9 to double stranded DNA and of thrombin to single stranded DNA. Since each DNA microarray copy was made with only 5 µl of spPCR mix, the whole process is cost-efficient. Hence, our DNA microarray copier has a great potential for becoming a standard lab tool.

Polymer-Templated Perylene-Probe Noncovalent Self-Assembly: A New Strategy for Label-Free Ultrasensitive Fluorescence Turn-On Biosensing

2013 ◽  
Vol 19 (38) ◽  
pp. 12846-12852 ◽  
Author(s):  
Yan Wang ◽  
Jian Chen ◽  
Huping Jiao ◽  
Yang Chen ◽  
Wenying Li ◽  
...  
Keyword(s):  
Self Assembly ◽  
Label Free ◽  
Turn On ◽  
New Strategy ◽  
Fluorescence Turn On

scholarly journals Design of a Fluorescence Turn-on and Label-free Aptasensor Using the Intrinsic Quenching Power of G-Quadruplex to AMT

2020 ◽  
Vol 36 (8) ◽  
pp. 965-970
Author(s):  
Dandan WANG ◽  
Fenghua GENG ◽  
Yongxiang WANG ◽  
Yu MA ◽  
Guixin LI ◽  
...  
Keyword(s):  
Label Free ◽  
Turn On ◽  
G Quadruplex ◽  
Fluorescence Turn On

A label-free fluorescence turn-on sensor for rapid detection of cysteine

Talanta ◽  
2015 ◽  
Vol 138 ◽  
pp. 144-148 ◽  
Author(s):  
Xia Chen ◽  
Hongli Liu ◽  
Chen Wang ◽  
Hui Hu ◽  
Yuhui Wang ◽  
...  
Keyword(s):  
Rapid Detection ◽  
Label Free ◽  
Turn On ◽  
Fluorescence Turn On

scholarly journals Graphene quantum dots based fluorescence turn-on nanoprobe for highly sensitive and selective imaging of hydrogen sulfide in living cells

2018 ◽  
Vol 6 (4) ◽  
pp. 779-784 ◽  
Author(s):  
Nan Li ◽  
Aung Than ◽  
Jie Chen ◽  
Fengna Xi ◽  
Jiyang Liu ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Hydrogen Sulfide ◽  
Real Time ◽  
Dynamic Change ◽  
Graphene Quantum Dots ◽  
Living Cells ◽  
Real Time Monitoring ◽  
Turn On ◽  
Highly Sensitive ◽  
Fluorescence Turn On

Graphene quantum dots-based fluorescent turn-on nanoprobe is developed for real-time monitoring the triggered dynamic change of intracellular H2S.

Nucleic acid-controlled quantum dots aggregation: A label-free fluorescence turn-on strategy for alkaline phosphatase detection

Talanta ◽  
2017 ◽  
Vol 169 ◽  
pp. 64-69 ◽  
Author(s):  
Zhenzhen Hu ◽  
Jian Chen ◽  
Yongxin Li ◽  
Yan Wang ◽  
Qingfeng Zhang ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Alkaline Phosphatase ◽  
Nucleic Acid ◽  
Label Free ◽  
Turn On ◽  
Fluorescence Turn On
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