Resonance scattering spectral detection of trace ATP based on label-free aptamer reaction and nanogold catalysis

The Analyst ◽  
2011 ◽  
Vol 136 (21) ◽  
pp. 4514 ◽  
Author(s):  
Aihui Liang ◽  
Huixiang Ouyang ◽  
Zhiliang Jiang
Keyword(s):  
Resonance Scattering ◽  
Label Free ◽  
Spectral Detection ◽  
Nanogold Catalysis

A Simple and Sensitive Label‐free Immunoassay for Factor B Using Resonance Scattering Spectral Detection

2012 ◽  
Vol 30 (7) ◽  
pp. 1636-1640 ◽  
Author(s):  
Jishun Li ◽  
Lili Xu ◽  
Lisheng Wang ◽  
Aihui Liang ◽  
Zhiliang Jiang
Keyword(s):  
Resonance Scattering ◽  
Label Free ◽  
Factor B ◽  
Spectral Detection

Ultrasensitive Resonance Scattering (RS) Spectral Detection for Trace Tetracycline in Milk Using Aptamer-Coated Nanogold (ACNG) as a Catalyst

2014 ◽  
Vol 62 (5) ◽  
pp. 1032-1037 ◽  
Author(s):  
Yanfang Luo ◽  
Lan He ◽  
Shenshan Zhan ◽  
Yuangen Wu ◽  
Le Liu ◽  
...  
Keyword(s):  
Resonance Scattering ◽  
Spectral Detection

Resonance Scattering Spectral Detection of Trace Pb2+ Using Aptamer-Modified AuPd Nanoalloy as Probe

Plasmonics ◽  
2010 ◽  
Vol 5 (4) ◽  
pp. 375-381 ◽  
Author(s):  
Zhiliang Jiang ◽  
Yanyan Fan ◽  
Aihui Liang ◽  
Guiqing Wen ◽  
Qingye Liu ◽  
...  
Keyword(s):  
Resonance Scattering ◽  
Spectral Detection

Resonance scattering spectral detection of ultratrace Hg(II) using herring sperm DNA modified nanogold probe as catalyst

Luminescence ◽  
2009 ◽  
Vol 25 (5) ◽  
pp. 373-377 ◽  
Author(s):  
Guiqing Wen ◽  
Aihui Liang ◽  
Zhiliang Jiang ◽  
Xianjiu Liao ◽  
Jianfu Li ◽  
...  
Keyword(s):  
Resonance Scattering ◽  
Sperm Dna ◽  
Spectral Detection

Resonance Scattering Spectral Detection of Trace Hg2+Using Aptamer-Modified Nanogold as Probe and Nanocatalyst

2009 ◽  
Vol 81 (13) ◽  
pp. 5439-5445 ◽  
Author(s):  
Zhiliang Jiang ◽  
Yanyan Fan ◽  
Menglin Chen ◽  
Aihui Liang ◽  
Xianjiu Liao ◽  
...  
Keyword(s):  
Resonance Scattering ◽  
Spectral Detection

scholarly journals Nanogold Catalysis–Based Immunoresonance-Scattering Spectral Assay for Trace Complement Component 3

2008 ◽  
Vol 54 (1) ◽  
pp. 116-123 ◽  
Author(s):  
Zhiliang Jiang ◽  
Wenxing Huang ◽  
Jiangping Li ◽  
Mingshun Li ◽  
Aihui Liang ◽  
...  
Keyword(s):  
Human Serum ◽  
Catalytic Effect ◽  
Linear Regression Analysis ◽  
High Sensitivity ◽  
Resonance Scattering ◽  
Complement Component ◽  
Serum Samples ◽  
Citrate Buffer ◽  
Nanogold Catalysis ◽  
Complement Component 3

Abstract Background: Complement component 3 (C3) is an essential bridge linking innate immunity and adaptive immunity. We describe an immunonanogold catalytic resonance-scattering (RS) technique for assaying C3 in serum. Methods: We used nanogold to label goat antihuman C3 antibody to obtain an immunonanogold RS probe for C3. The immune reaction between nanogold-labeled antibodies and antigens was carried out in Na2HPO4–sodium citrate buffer, pH 5.6, containing polyethylene glycol. After centrifuging the particle suspension, we used RS to monitor the catalytic effect of nanogold-labeled anti-C3 in the supernatant on the chlorauric acid–hydroxylamine (HAuCl4–NH2OH) particle reaction and used electron microscopy to monitor particle shape. We assayed 36 human serum samples with the immunonanogold catalytic RS assay and immunoturbidimetry. Results: Nanogold-labeled anti-C3 had a marked catalytic effect on the reaction of HAuCl4 and NH2OH to form particles, which exhibit a maximum RS peak at 585 nm. The decrease in RS intensity, ΔIRS, of the nanocatalytic system was proportional to C3 concentration from 5.0 to 160.0 ng/L. The detection limit for the C3 assay was 1.52 ng/L. Results obtained with serum samples agreed with those obtained with an immunoturbidimetric method. A linear regression analysis of 28 nonpathologic serum samples revealed a correlation coefficient of 0.960, with mean (SD) slope and intercept values of 0.787 (0.0218) g/L and 0.28 (0.026) g/L C3, respectively. Conclusion: The immunonanogold catalytic RS assay showed high sensitivity and good selectivity for measuring C3 in human serum. This method may become useful for diagnosing certain diseases, such as hepatitis.

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