Microfluidic device for analyzing preferential chemotaxis and chemoreceptor sensitivity of bacterial cells toward carbon sources

The Analyst ◽  
2011 ◽  
Vol 136 (16) ◽  
pp. 3238 ◽  
Author(s):  
Minseok Kim ◽  
Su Hyun Kim ◽  
Sung Kuk Lee ◽  
Taesung Kim
Keyword(s):  
Microfluidic Device ◽  
Carbon Sources ◽  
Bacterial Cells

Significance of External Carbon Sources on Simultaneous Removal of Nutrients from Wastewater

1992 ◽  
Vol 26 (5-6) ◽  
pp. 1047-1055 ◽  
Author(s):  
N. F. Y. Tam ◽  
Y. S. Wong ◽  
G. Leung
Keyword(s):  
Sodium Acetate ◽  
Batch Reactor ◽  
Inorganic Nitrogen ◽  
Carbon Sources ◽  
Simultaneous Removal ◽  
Bacterial Cells ◽  
High Concentration ◽  
P Content ◽  
Carbon Substrates ◽  
Effective Source

Laboratory-scale studies were undertaken to examine the effects of easily-biodegradable organic substances upon the nutrient removal by a simulated sequencing batch reactor (SBR). The fill and react period of the SBR was 14 hours, including an instant fill, 7 hours aeration, 4 hours anoxic and 3 hours aeration period. Three kinds of commonly used carbon sources, namely methanol, glucose and sodium acetate, at the concentrations equivalent to theoretical COD values of 50, 100 and 150 mg O2 l-1 were added to each reactor prior to the anoxic stage. The results showed that the concentration of NH4+-N dropped from its initial 50 to 18 mg l-1 (64 % removal) during the first aeration period, with the NO3−-N content increased from 2 to 33 mg l−1. A 60% depletion of COD was also recorded in this period. Denitrification occurred during the anoxic period, higher amount of NO3−1-N was removed in the reactors supplemented with carbon substrates at the concentrations of 100 and 150 mg l-1. The final inorganic nitrogen content was less than 5 mg l-1 in the reactor supplemented with 150 mg l-1 sodium acetate. Simultaneous removal of phosphorus was reported in reactors supplied with high concentration of sodium acetate. In these reactors, large amount of P was released during the anoxic/anaerobic period but the released P was taken up by bacterial cells in the subsequent aeration stage, and the final P content was less than 1.5 mg l-1 (84 % removal was achieved). Among the three carbon sources used, sodium acetate was the most efficient and effective source in removing wastewater nutrients, followed by methanol, and glucose was the least reliable substrate.

scholarly journals Microfluidic Device for Automated Synchronization of Bacterial Cells

2012 ◽  
Vol 84 (20) ◽  
pp. 8571-8578 ◽  
Author(s):  
Seth M. Madren ◽  
Michelle D. Hoffman ◽  
Pamela J. B. Brown ◽  
David T. Kysela ◽  
Yves V. Brun ◽  
...  
Keyword(s):  
Microfluidic Device ◽  
Bacterial Cells

Some Physiological Characteristics of Acinetobacter spp. Accumulating Large Amounts of Phosphate

1985 ◽  
Vol 17 (11-12) ◽  
pp. 119-125 ◽  
Author(s):  
Maria H. Deinema ◽  
Mark van Loosdrecht ◽  
Annie Scholten
Keyword(s):  
Dry Matter ◽  
Phosphate Uptake ◽  
Carbon Sources ◽  
Bacterial Cells ◽  
Anaerobic Conditions ◽  
Carbon And Nitrogen ◽  
P Content ◽  
Acinetobacter Strain ◽  
Acinetobacter Spp ◽  
Acetate Medium

Acinetobacter strain 210A has been grown in batch and continuous cultures in an acetate medium. The P content of the cells varied from 6–10% of the dry matter and phosphate uptake continued for months as long as carbon and nitrogen source, inorganic salts and oxygen were present. The growing ( = metabolizing) bacterial cells released phosphate under anaerobic conditions. Addition of CO2, acetate and ethanol or other reduced carbon sources stimulated the rate of the phosphate release but after 24 hours of anaerobiosis the obtained results with or without additions were similar.

Rapid quantification of bacterial cells in potable water using a simplified microfluidic device

2007 ◽  
Vol 68 (3) ◽  
pp. 643-647 ◽  
Author(s):  
Chieko Sakamoto ◽  
Nobuyasu Yamaguchi ◽  
Masumi Yamada ◽  
Hiroyasu Nagase ◽  
Minoru Seki ◽  
...  
Keyword(s):  
Microfluidic Device ◽  
Potable Water ◽  
Bacterial Cells ◽  
Rapid Quantification

scholarly journals Statistical optimization of the lysis agents for gram-negative bacterial cells in a microfluidic device

2006 ◽  
Vol 11 (6) ◽  
pp. 557-557
Author(s):  
Young-Bum Kim ◽  
Ji-Ho Park ◽  
Woo-Jin Chang ◽  
Yoon-Mo Koo ◽  
Eun-Ki Kim ◽  
...  
Keyword(s):  
Microfluidic Device ◽  
Statistical Optimization ◽  
Bacterial Cells ◽  
Gram Negative

scholarly journals Rapid, Semiautomated Quantification of Bacterial Cells in Freshwater by Using a Microfluidic Device for On-Chip Staining and Counting

2010 ◽  
Vol 77 (4) ◽  
pp. 1536-1539 ◽  
Author(s):  
Nobuyasu Yamaguchi ◽  
Masashi Torii ◽  
Yuko Uebayashi ◽  
Masao Nasu
Keyword(s):  
Escherichia Coli ◽  
Microfluidic Device ◽  
Potable Water ◽  
Pond Water ◽  
Bacterial Cells ◽  
On Chip

ABSTRACTA microfluidic device-based system for the rapid and semiautomated counting of bacteria in freshwater was fabricated and examined. Bacteria in groundwater and in potable water, as well as starvedEscherichia coliO157:H7 spiked in pond water, were able to be on-chip stained and enumerated within 1 h using this system.

3,4-Dichlorobenzoic acid biodegradation by the Edwardsiella tarda: Effect of Some Growth Conditions

2020 ◽  
Vol 6 (1) ◽  
pp. 7-14
Author(s):  
Ali Alqudah ◽  
Waleed Jaafreh
Keyword(s):  
Cell Mass ◽  
Carbon Sources ◽  
Nitrogen Sources ◽  
Growth Conditions ◽  
Edwardsiella Tarda ◽  
Ring Cleavage ◽  
Bacterial Cells ◽  
Carbon And Nitrogen ◽  
Catechol 1,2 Dioxygenase ◽  
Degradation Ability

The biodegradation of 3,4-DiChlorobenzoic acid was investigated by using Edwardsiella tarda and it used 3,4-DCBA as sole carbon and energy source. Several concentrations of 3,4-D CBAs (1mM, 2mM ,3mM ,4mM and 5mM) were used. The highest rate of degradation of 3,4-D CBAs was obtained at a concentration (2mM). The experiments were included substrate concentration, temperature, pH, starvation, adaptation, carbon and nitrogen sources. The degradation ability was monitored through the release of chloride disappearance of the substrate and finally the growth of bacterial cells on that substrate. The optimal temperature and pH for the bacteria were 42ºC and 7.5, respectively. Adaptation of the cells on 3,4-DCBA for 48 hours and cells starvation for 24 hours and 48 hours increasing the initial degradation rate. The carbon sources affected the 3,4 –DCBA degradation differently from that on chloride and cell mass production. Nitrogen sources supplied (yeast extract, L-proline, casein, NH4, K-Nitrate, arginine, urea and glycine). Urea and casine caused a repression in 3,4-DCBA degradation. Catechol 1,2 dioxygenase activity was found to be present in cell free extracts suggesting that 3,4-DCBA is catabolized by ortho-ring cleavage pathway.

Biodegradation of Chlorobenzoic Acid Substitutes, Particularly, 2- Chlorobenzoic Acid by Aeromonas hydrophila

2019 ◽  
Vol 5 (2) ◽  
pp. 124-135
Author(s):  
Khaled Tarawneh ◽  
Farah AL-Quraishi ◽  
Haitham Qaralleh ◽  
Amjad Al Tarawneh ◽  
Muhamad Al-limoun ◽  
...  
Keyword(s):  
Aeromonas Hydrophila ◽  
Cell Mass ◽  
Carbon Sources ◽  
Treatment Plant ◽  
Nitrogen Sources ◽  
Inoculum Size ◽  
Ring Cleavage ◽  
Enhancement Effect ◽  
Bacterial Cells ◽  
Chlorobenzoic Acid

Bacterium Aeromonas hydrophila (A. hydrophila) was isolated from the Petra Wastewater Treatment Plant effluent in southern Jordan. It was identified by using morphological and biochemical characteristics. A. hydrophila was found to be able of using chlorobenzoate compounds as carbon and energy source. These capabilities were with different biodegradation rates (4- chlorobenzoic acid 5µM/hr, 3,4-dichlorobenzoic acid 15.5µM/hr, 2- chlorobenzoic acid 41µM/hr and 3- chlorobenzoic acid 65µM/hr). The degradation ability was monitored through the release of chloride, disappearance of the substrate and finally the growth of bacterial cells on these substrates. A. hydrophila dioxygenases physiologically induced by chlorobenzoic acid compounds, were analyzed for both ortho or meta ring-cleavage of these aromatic compounds. Only 1, 2-dioxygenase activity was detected which suggest that the cleavage is through the ortho pathway. The best results of degradation of 2-CBA compound were obtained with 3mM substrate concentration, 25ºC, pH 7and 200µl inoculum size. The carbon sources affected the 2-CBA degradation differently from that on chloride and cell mass production. Nitrogen sources used reduced the degradation activity of the 2-CBA as well as in the chlorine release from 2-CBA. However, the nitrogen source L-proline had a slight enhancement effect on the biodegradation between the 40-80h.

THE UTILIZATION OF CARBON SOURCES MIXTURE (GLUCOSE, GLYCEROL AND FORMATE) AND GENERATION OF FERMENTATION END-PRODUCTS BY ESCHERICHIA COLI

2020 ◽  
Vol 54 (1 (251)) ◽  
pp. 55-62
Author(s):  
H.Kh. Gevorgyan
Keyword(s):  
Escherichia Coli ◽  
Carbon Sources ◽  
Bacterial Cells ◽  
E Coli ◽  
End Products ◽  
Glycerol Utilization ◽  
First Time ◽  
External Ph

In this study anaerobic utilization of mixed carbon sources (glucose, glycerol, formate) and generation of fermentation end-products by Escherichia coli at slightly alkaline and slightly acidic pHs was investigated at the first time. It has been shown that E. coli is able to perform co-fermentation of glucose and glycerol in the presence of external formate. The latter was utilized by bacterial cells at first. Acetate is the permanent product (25–50 mM) during both glucose and glycerol utilization. It has been revealed that composition of fermentation end-products depends not only on external pH, but also on co-utilization of substrates.

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