Triple substitution G216N/A217L/S398M leads to the active and thermostable Luciola mingrelica firefly luciferase

Mikhail I. Koksharov; Natalia N. Ugarova

doi:10.1039/c0pp00318b

Triple substitution G216N/A217L/S398M leads to the active and thermostable Luciola mingrelica firefly luciferase

Photochemical & Photobiological Sciences ◽

10.1039/c0pp00318b ◽

2011 ◽

Vol 10 (6) ◽

pp. 931 ◽

Cited By ~ 16

Author(s):

Mikhail I. Koksharov ◽

Natalia N. Ugarova

Keyword(s):

Firefly Luciferase ◽

Luciola Mingrelica

Download Full-text

ENHANCEMENT OF THERMOSTABILITY OF LUCIOLA MINGRELICA FIREFLY LUCIFERASE BY MUTAGENESIS OF NON-CONSERVATIVE RESIDUES CYS62 AND CYS146

Bioluminescence and Chemiluminescence ◽

10.1142/9789812839589_0009 ◽

2008 ◽

Author(s):

G. Y. LOMAKINA ◽

Y. A. MODESTOVA ◽

N. N. UGAROVA

Keyword(s):

Firefly Luciferase ◽

Luciola Mingrelica

Download Full-text

COMPARISON OF KINETIC PROPERTIES OF FIREFLY LUCIFERASE FROM PHOTINUS PYRALIS AND LUCIOLA MINGRELICA

Bioluminescence and Chemiluminescence ◽

10.1142/9789812702203_0011 ◽

2005 ◽

Cited By ~ 1

Author(s):

LY BROVKO ◽

OA GANDELMAN ◽

IB KERSHENGOLZ ◽

NN UGAROVA

Keyword(s):

Firefly Luciferase ◽

Kinetic Properties ◽

Photinus Pyralis ◽

Luciola Mingrelica

Download Full-text

Quenching of the fluorescence of Tyr and Trp residues of firefly luciferase from Luciola mingrelica by the substrates

Biochemistry (Moscow) ◽

10.1134/s0006297907090064 ◽

2007 ◽

Vol 72 (9) ◽

pp. 962-967 ◽

Cited By ~ 4

Author(s):

T. N. Vlasova ◽

N. N. Ugarova

Keyword(s):

Firefly Luciferase ◽

Luciola Mingrelica

Download Full-text

Enhancement of thermostability of the Luciola mingrelica firefly luciferase by site-directed mutagenesis of nonconservative cysteine residues Cys62 and Cys146

Moscow University Chemistry Bulletin ◽

10.3103/s002713140802003x ◽

2008 ◽

Vol 63 (2) ◽

pp. 63-66 ◽

Cited By ~ 1

Author(s):

G. Yu. Lomakina ◽

Yu. A. Modestova ◽

N. N. Ugarova

Keyword(s):

Firefly Luciferase ◽

Site Directed Mutagenesis ◽

Cysteine Residues ◽

Directed Mutagenesis ◽

Luciola Mingrelica

Download Full-text

Stabilization of Luciola mingrelica firefly luciferase by genetic engineering methods

Moscow University Chemistry Bulletin ◽

10.3103/s0027131410030065 ◽

2010 ◽

Vol 65 (3) ◽

pp. 139-143 ◽

Cited By ~ 1

Author(s):

N. N. Ugarova

Keyword(s):

Genetic Engineering ◽

Firefly Luciferase ◽

Luciola Mingrelica

Download Full-text

Site-directed mutagenesis of cysteine residues of Luciola mingrelica firefly luciferase

Biochemistry (Moscow) ◽

10.1134/s0006297911100087 ◽

2011 ◽

Vol 76 (10) ◽

pp. 1147-1154 ◽

Cited By ~ 5

Author(s):

Yu. A. Modestova ◽

G. Yu. Lomakina ◽

N. N. Ugarova

Keyword(s):

Firefly Luciferase ◽

Site Directed Mutagenesis ◽

Cysteine Residues ◽

Directed Mutagenesis ◽

Luciola Mingrelica

Download Full-text

CATALYTIC PROPERTIES AND BIOLUMINESCENCE SPECTRA OF RECOMBINANT FIREFLY LUCIFERASE LUCIOLA MINGRELICA WITH POINT MUTATIONS OUT OF THE ENZYME ACTIVE SITE

Bioluminescence and Chemiluminescence ◽

10.1142/9789812776624_0009 ◽

2002 ◽

Author(s):

L G MALOSHENOK ◽

N N UGAROVA

Keyword(s):

Active Site ◽

Catalytic Properties ◽

Point Mutations ◽

Firefly Luciferase ◽

Enzyme Active Site ◽

Luciola Mingrelica

Download Full-text

INTERACTION OF FIREFLY LUCIFERASE LUCIOLA MINGRELICA WITH DIMETHYLOXYLUCIFERIN

Bioluminescence and Chemiluminescence ◽

10.1142/9789812776624_0008 ◽

2002 ◽

Author(s):

O V LEONTIEVA ◽

T N VLASOVA ◽

N N UGAROVA ◽

D WEISS

Keyword(s):

Firefly Luciferase ◽

Luciola Mingrelica

Download Full-text

STABILIZATION OF LUCIOLA MINGRELICA FIREFLY LUCIFERASE BY MUTATION OF NON-CONSERVATIVE CYSTEINE RESIDUES

Bioluminescence and Chemiluminescence ◽

10.1142/9789812770196_0004 ◽

2007 ◽

Author(s):

G. Y. LOMAKINA ◽

S. A. LEGOTSKY ◽

V. I. TISHKOV ◽

N. N. UGAROVA

Keyword(s):

Firefly Luciferase ◽

Cysteine Residues ◽

Luciola Mingrelica

Download Full-text

Random mutagenesis of Luciola mingrelica firefly luciferase. Mutant enzymes with bioluminescence spectra showing low pH sensitivity

Biochemistry (Moscow) ◽

10.1134/s0006297908080038 ◽

2008 ◽

Vol 73 (8) ◽

pp. 862-869 ◽

Cited By ~ 10

Author(s):

M. I. Koksharov ◽

N. N. Ugarova

Keyword(s):

Random Mutagenesis ◽

Firefly Luciferase ◽

Low Ph ◽

Ph Sensitivity ◽

Luciola Mingrelica

Download Full-text