scholarly journals Optimizing photodynamic therapy by liposomal formulation of the photosensitizer pyropheophorbide-a methyl ester: In vitro and ex vivo comparative biophysical investigations in a colon carcinoma cell line

2010 ◽  
Vol 9 (9) ◽  
pp. 1252 ◽  
Author(s):  
Pierre-Henri Guelluy ◽  
Marie-Pierre Fontaine-Aupart ◽  
Angeliki Grammenos ◽  
Sandrine Lécart ◽  
Jacques Piette ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Methyl Ester ◽  
Cell Line ◽  
Colon Carcinoma ◽  
Carcinoma Cell ◽  
Ex Vivo ◽  
Liposomal Formulation ◽  
Colon Carcinoma Cell Line ◽  
Pyropheophorbide A

scholarly journals Are Compounds Membrane-Associated or Present in the Cytosol? A Study Using Polyphenols in a Colon Carcinoma Cell Line Model

2020 ◽  
Vol 6 (6) ◽  
pp. 451-456
Author(s):  
Hannah Deusser ◽  
Isabel Groh ◽  
Tamara Bakuradze ◽  
Nadine Simson ◽  
Eva Kaiser ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Cell Line ◽  
Colon Carcinoma ◽  
Carcinoma Cell ◽  
Carcinoma Cell Line ◽  
Biologically Active ◽  
Colon Carcinoma Cell Line ◽  
Cell Line Model ◽  
Apple Products

Abstract Purpose We investigated the cytosolic and membrane-associated contents of polyphenols after 4 hours of incubation (50 μM of each polyphenol) in the colon carcinoma cell line T84 using a novel, rapid, and convenient method based on permeabilization of the cell membrane using digitonin. The colon carcinoma cell line was used to investigate the intestinal uptake of polyphenols present in apple products. Recent Findings The results showed that hydroxycinnamic acids (caffeic and 5-caffeoylquinic acid) were only detected in the cytosolic fractions. In contrast, 0.3 to 8.2% of the initial concentrations (50 μM) of the flavonoids phloretin, quercetin, phloretin 2′-O-glucoside, and quercetin 3-O-rhamnoside were found in the membrane-associated fractions. In the cytosolic fractions, 0.2–2.9% of these compounds were detected, corresponding to 25 to 40% of the total cell-associated (cytosolic plus membrane-associated fractions) polyphenol content. Summary Our results showed that after uptake, polyphenols were present in the cytosolic fraction of the cells as well as associated with the cell membrane. The presented method provides a useful in vitro tool for determining biologically active compounds in cellular fractions.

Anticancer effects of amooranin in human colon carcinoma cell line in vitro and in nude mice xenografts

2006 ◽  
Vol 119 (10) ◽  
pp. 2443-2454 ◽  
Author(s):  
Cheppail Ramachandran ◽  
P.K. Raveendran Nair ◽  
Arturo Alamo ◽  
Curtis Bruce Cochrane ◽  
Enrique Escalon ◽  
...  
Keyword(s):  
Cell Line ◽  
Colon Carcinoma ◽  
Nude Mice ◽  
Carcinoma Cell ◽  
Human Colon ◽  
Human Colon Carcinoma Cell ◽  
Colon Carcinoma Cell Line ◽  
Anticancer Effects ◽  
Human Colon Carcinoma

Synthesis of galactoglycerolipids found in the HT29 human colon carcinoma cell line

Tetrahedron ◽  
2002 ◽  
Vol 58 (25) ◽  
pp. 5109-5117 ◽  
Author(s):  
Jan Lindberg ◽  
Stefan C.T Svensson ◽  
Peter Påhlsson ◽  
Peter Konradsson
Keyword(s):  
Cell Line ◽  
Colon Carcinoma ◽  
Carcinoma Cell ◽  
Human Colon ◽  
Carcinoma Cell Line ◽  
Human Colon Carcinoma Cell ◽  
Colon Carcinoma Cell Line ◽  
Human Colon Carcinoma
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