Structural characterization of the core region from the lipopolysaccharide of the haloalkaliphilic bacterium Halomonas alkaliantarctica strain CRSS

2010 ◽  
Vol 8 (23) ◽  
pp. 5404 ◽  
Author(s):  
Giuseppina Pieretti ◽  
Sara Carillo ◽  
Barbara Nicolaus ◽  
Annarita Poli ◽  
Rosa Lanzetta ◽  
...  
Keyword(s):  
Structural Characterization ◽  
Core Region ◽  
The Core

Structural Characterization of the Core Region of the Lipopolysaccharide from the HaloalkaliphilicHalomonas pantelleriensis: Identification of the Biological O-Antigen Repeating Unit

2008 ◽  
Vol 2008 (4) ◽  
pp. 721-728 ◽  
Author(s):  
Giuseppina Pieretti ◽  
Maria Michela Corsaro ◽  
Rosa Lanzetta ◽  
Michelangelo Parrilli ◽  
Barbara Nicolaus ◽  
...  
Keyword(s):  
Structural Characterization ◽  
Core Region ◽  
The Core ◽  
O Antigen

scholarly journals Synthesis and structural characterization of hexa-μ2-chlorido-μ4-oxido-tetrakis{[4-(phenylethynyl)pyridine-κN]copper(II)} dichloromethane monosolvate

2021 ◽  
Vol 77 (1) ◽  
pp. 42-46
Author(s):  
Rayya A. Al Balushi ◽  
Muhammad S. Khan ◽  
Md. Serajul Haque Faizi ◽  
Ashanul Haque ◽  
Kieran Molloy ◽  
...  
Keyword(s):  
Molecular Structure ◽  
Crystal Structure ◽  
Structural Characterization ◽  
Title Compound ◽  
Three Dimensional ◽  
Hirshfeld Surface ◽  
The Core ◽  
Dimensional Network ◽  
Packing Arrangement

In the crystal structure of the title compound, [Cu4Cl6O(C13H9N)4]·CH2Cl2, the core molecular structure consists of a Cu4 tetrahedron with a central interstitial O atom. Each edge of the Cu4 tetrahedron is bridged by a chlorido ligand. Each copper(II) cation is coordinated to the central O atom, two chlorido ligands and one N atom of the 4-phenylethynylpyridine ligand. In the crystal, the molecules are linked by intermolecular C—H...Cl interactions. Furthermore, C—H...π and π–π interactions also connect the molecules, forming a three-dimensional network. Hirshfeld surface analysis indicates that the most important contributions for the packing arrangement are from H...H and C...H/H...C interactions.

scholarly journals Characterization of rifampicin-resistant Mycobacterium tuberculosis in Taiwan

2003 ◽  
Vol 52 (3) ◽  
pp. 239-245 ◽  
Author(s):  
Hsing-Yu Hwang ◽  
Chung-Yu Chang ◽  
Lin-Li Chang ◽  
Shui-Feng Chang ◽  
Ya-Hui Chang ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Core Region ◽  
Banding Patterns ◽  
The Core ◽  
Resistant Tuberculosis ◽  
Proportion Method ◽  
The Mean ◽  
Novel Alleles ◽  
The Relationship

Sixty-three rifampicin-resistant (Rifr) isolates of Mycobacterium tuberculosis from Kaohsiung, Taiwan, were analysed for mutations in the core region (69 bp, codons 511–533) of the rpoB gene. Some 84.1 % (53/63) of the resistant isolates showed mutations in this region, especially in codons 531 (41.5 %), 526 (18.9 %), 516 (15.1 %) and 533 (7.5 %). Five novel alleles of a total of 16 different types of mutations were identified in Rifr isolates. Ten Rifr isolates (15.9 %) exhibited no mutations in the core region of rpoB. Also, they did not show mutations in another 365 bp fragment (codons 99–220) of rpoB. The agar proportion method was used to determine the relationship between the degree of rifampicin resistance and alterations in the core region of rpoB. The results revealed that the mean MIC was 92.38 μg ml−1 for the 53 isolates with a mutation in the core region, whereas the mean MIC of the other 10 isolates without mutations was only 24.8 μg ml−1. This indicates that the isolates with mutations in the core region had higher levels of resistance than those without mutations in this region. IS6110 restriction fragment length polymorphism (RFLP) was used for typing of 55 Rifr M. tuberculosis isolates. Isolates contained two to 19 copies of IS6110, with sizes ranging from 600 to 16 000 bp. The majority (85 %) contained six to 16 copies. No strains lacking IS6110 were found. A total of 54 of 55 RFLP types were defined at the 90 % similarity level. The observation of varied IS6110-associated banding patterns indicates that an outbreak of drug-resistant tuberculosis did not occur in this area.

scholarly journals Structural and Computational Characterization of Disease-Related Mutations Involved in Protein-Protein Interfaces

2019 ◽  
Vol 20 (7) ◽  
pp. 1583 ◽  
Author(s):  
Dàmaris Navío ◽  
Mireia Rosell ◽  
Josu Aguirre ◽  
Xavier de la Cruz ◽  
Juan Fernández-Recio
Keyword(s):  
Protein Interactions ◽  
Computational Analysis ◽  
Molecular Level ◽  
Binding Free Energy ◽  
Evolutionary Conservation ◽  
Core Region ◽  
Protein Protein Interactions ◽  
The Core ◽  
Protein Interfaces

One of the known potential effects of disease-causing amino acid substitutions in proteins is to modulate protein-protein interactions (PPIs). To interpret such variants at the molecular level and to obtain useful information for prediction purposes, it is important to determine whether they are located at protein-protein interfaces, which are composed of two main regions, core and rim, with different evolutionary conservation and physicochemical properties. Here we have performed a structural, energetics and computational analysis of interactions between proteins hosting mutations related to diseases detected in newborn screening. Interface residues were classified as core or rim, showing that the core residues contribute the most to the binding free energy of the PPI. Disease-causing variants are more likely to occur at the interface core region rather than at the interface rim (p < 0.0001). In contrast, neutral variants are more often found at the interface rim or at the non-interacting surface rather than at the interface core region. We also found that arginine, tryptophan, and tyrosine are over-represented among mutated residues leading to disease. These results can enhance our understanding of disease at molecular level and thus contribute towards personalized medicine by helping clinicians to provide adequate diagnosis and treatments.

scholarly journals Structural characterization of the core of the paired helical filament of Alzheimer disease.

1988 ◽  
Vol 85 (13) ◽  
pp. 4884-4888 ◽  
Author(s):  
C. M. Wischik ◽  
M. Novak ◽  
P. C. Edwards ◽  
A. Klug ◽  
W. Tichelaar ◽  
...  
Keyword(s):  
Alzheimer Disease ◽  
Structural Characterization ◽  
Paired Helical Filament ◽  
The Core

scholarly journals Characterization of Samples Identified as Hepatitis C Virus Genotype 1 without Subtype by Abbott RealTime HCV Genotype II Assay Using the New Abbott HCV GenotypePlusRUO Test

2015 ◽  
Vol 54 (2) ◽  
pp. 296-299 ◽  
Author(s):  
Camelia Mokhtari ◽  
Anne Ebel ◽  
Birgit Reinhardt ◽  
Sandra Merlin ◽  
Stéphanie Proust ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Core Region ◽  
Genotype 1 ◽  
Virus Genotype ◽  
Hcv Genotype ◽  
The Core ◽  
Hcv Genotyping ◽  
Genotype Ii

Hepatitis C virus (HCV) genotyping continues to be relevant for therapeutic strategies. Some samples are reported as genotype 1 (gt 1) without subtype by the Abbott RealTime HCV Genotype II (GT II) test. To characterize such samples further, the Abbott HCV GenotypePlusRUO (Plus) assay, which targets the core region for gt 1a, gt 1b, and gt 6 detection, was evaluated as a reflex test in reference to NS5B or 5′-untranslated region (UTR)/core region sequencing. Of 3,626 routine samples, results of gt 1 without subtype were received for 171 samples (4.7%), accounting for 11.5% of gt 1 specimens. The Plus assay and sequencing were applied to 98 of those samples. NS5B or 5′-UTR/core region sequencing was successful for 91/98 specimens (92.9%). Plus assay and sequencing results were concordant for 87.9% of specimens (80/91 samples). Sequencing confirmed Plus assay results for 82.6%, 85.7%, 100%, and 89.3% of gt 1a, gt 1b, gt 6, and non-gt 1a/1b/6 results, respectively. Notably, 12 gt 6 samples that had been identified previously as gt 1 without subtype were assigned correctly here; for 25/28 samples reported as “not detected” by the Plus assay, sequencing identified the samples as gt 1 with subtypes other than 1a/1b. The genetic variability of HCV continues to present challenges for the current genotyping platforms regardless of the applied methodology. Samples identified by the GT II assay as gt 1 without subtype can be further resolved and reliably characterized by the new Plus assay.

Microstructural characterization of a SiC Whisker-Reinforced HIPped Reaction-Bonded Si3N4

1988 ◽  
Vol 120 ◽  
Author(s):  
S. C. Farmer ◽  
P. Pirouz ◽  
A. H. Heuer
Keyword(s):  
Grain Boundaries ◽  
Glassy Phase ◽  
Microstructural Characterization ◽  
Core Region ◽  
Sintering Aid ◽  
The Core ◽  
Composite Fabrication ◽  
The Matrix ◽  
Sic Whiskers

AbstractA SiC whisker reinforced HIPped RBSN material fabricated with a Y2O3 sintering aid was characterized using TEM. The matrix is > 90% β-Si3 N4 with a Y-Si-O-N glassy phase at the Si3 N4 grain boundaries and about the SiC whiskers. The SiC whisiers are heavily faulted and have a well defined core. Si3 N4 precipitates are observed in the core region after composite fabrication. A preliminary mechanism for the growth of the SiC whisker, based on the VLS mechanism is proposed.

scholarly journals Protein 4.1R self-association: identification of the binding domain

2006 ◽  
Vol 400 (3) ◽  
pp. 457-465
Author(s):  
Carmen M. Pérez-Ferreiro ◽  
Eva Lospitao ◽  
Isabel Correas
Keyword(s):  
Plasma Membrane ◽  
Core Region ◽  
Binding Domain ◽  
Intramolecular Interactions ◽  
Actin Network ◽  
Protein 4.1 ◽  
The Core ◽  
Self Association ◽  
Protein 4.1R

Erythroid protein 4.1 (4.1R) stabilizes the spectrin–actin network and anchors it to the plasma membrane. To contribute to the characterization of non-erythroid protein 4.1R, we used sedimentation, pull-down and co-immunoprecipitation assays to investigate the ability of protein 4.1R to establish inter-/intra-molecular associations. We demonstrated that the small 4.1R isoforms of 60 kDa (4.1R60), but not the larger isoforms of 80 and 135 kDa (4.1R80 and 4.1R135), were self-associated, and that a domain contained in all 4.1R isoforms, the core region, was responsible for 4.1R self-association. Results from denaturing–renaturing experiments, in which an initially non-self-associated 4.1R80 isoform became self-associated, suggested that an initially hidden core region was subsequently exposed. This hypothesis was supported by results from pull-down assays, which showed that the core region interacted with the N-terminal end of the FERM (4.1, ezrin, radixin, moesin) domain that is present in 4.1R80 and 4.1R135 isoforms but absent from 4.1R60 isoforms. Consistently, 4.1R80 isoforms bound neither to each other nor to 4.1R60 isoforms. We propose that 4.1R60 isoforms are constitutively self-associated, whereas 4.1R80 and 4.1R135 self-association is prevented by intramolecular interactions.

Experimental investigation and simulation of the performance of a pre-twister based on the jet vortex field used for the ring spinning frame

2019 ◽  
Vol 89 (21-22) ◽  
pp. 4647-4657
Author(s):  
Shanshan He ◽  
Wenliang Xue ◽  
Ge Chen ◽  
Longdi Cheng
Keyword(s):  
Core Region ◽  
Ring Spinning ◽  
Air Velocity ◽  
The Core ◽  
Yarn Properties ◽  
Airflow Field ◽  
Movement And Deformation ◽  
Simulation Results ◽  
Spinning Speed

The ring spun technique has always held a domain position in yarn spinning, thanks to its simple and robust structure, ease of maintenance and good quality in the product. A pre-twist device is arranged between the front rollers and an additional pair of rollers, which are named the holding rollers. In this paper, the simulation and characterization of the airflow field, as well as the fiber movement and deformation, in this pre-twisting zone are carried out by computational fluid dynamics software. The airflow simulation results show that there is a core region formed around the roving in the pre-twister, with a radius of about 1.55 mm. In the core region, the tangential speed is the major velocity component ranging from 0–70 m/s and the axial velocity is the minor one ranging from –13 to about 4.1 m/s. The air velocity inside the whole tube is from 462 to 0 m/s. The two-way coupled simulation verified that the airflow can drive the fibers to wind on the roving. The experiments on the surface morphology verified the wrapping phenomena in the simulation. Due to the employment of the pre-twister, the yarn properties got a little worse, with declines of 6% in strength and 10% in evenness, respectively. Comparing with the Uster Statistics for 2018, these declines are acceptable. Furthermore, it is shown that with this kind of pre-twister, the spinning speed can increase by 27.13% with an acceptable decrease in cotton yarn property (decreases of 13% in strength and 8% in evenness).

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