A microfluidic approach for in vitro assessment of interorgan interactions in drug metabolism using intestinal and liver slices

Lab on a Chip ◽  
2010 ◽  
Vol 10 (20) ◽  
pp. 2778 ◽  
Author(s):  
Paul M. van Midwoud ◽  
Marjolijn T. Merema ◽  
Elisabeth Verpoorte ◽  
Geny M. M. Groothuis
Keyword(s):  
Drug Metabolism ◽  
Liver Slices ◽  
In Vitro Assessment

Application of Capillary Electrophoresis To the In Vitro Assessment of Drug Metabolism

1995 ◽  
Vol 23 (3) ◽  
pp. 432S-432S
Author(s):  
CM Hill ◽  
KE Herbert ◽  
MD Evans ◽  
J Lunec
Keyword(s):  
Capillary Electrophoresis ◽  
Drug Metabolism ◽  
In Vitro Assessment

In vitro assessment of hepatic drug metabolism in man: a clinical pharmacological perspective

1982 ◽  
Vol 3 ◽  
pp. 487-490 ◽  
Author(s):  
Christer von Bahr ◽  
Hans Glaumann ◽  
Britt Mellström ◽  
Folke Sjöqvist
Keyword(s):  
Drug Metabolism ◽  
Hepatic Drug Metabolism ◽  
Hepatic Drug ◽  
In Vitro Assessment

In vitro assessment of Labisia pumila and its constituents for interactions with CYPs, P-GP and PXR

Planta Medica ◽  
2014 ◽  
Vol 80 (10) ◽  
Author(s):  
VK Manda ◽  
OR Dale ◽  
C Awortwe ◽  
Z Ali ◽  
IA Khan ◽  
...  
Keyword(s):  
In Vitro Assessment ◽  
Labisia Pumila

CORTICOSTEROID-UMSATZ UND CORTICOSTEROID-PRODUKTION BEI RATTEN UNTER DER BEHANDLUNG MIT ANABOLEN STEROIDEN

1965 ◽  
Vol 48 (2) ◽  
pp. 263-271 ◽  
Author(s):  
Herbert Schriefers ◽  
Gerlinde Scharlau ◽  
Franzis Pohl
Keyword(s):  
Production Rate ◽  
Adult Female ◽  
Anabolic Steroids ◽  
Reduction Rate ◽  
Female Rats ◽  
Adrenal Weight ◽  
Hydrogenase Activity ◽  
Anabolic Effect ◽  
Liver Slices

ABSTRACT After the administration of anabolic steroids to adult female rats in daily doses of 1 mg per animal for 14 days, the following parameters were investigated: the rate of the Δ4-5α-hydrogenase-catalyzed cortisone reduction in liver slices and microsomal fractions, the adrenal weight and the in vitro corticosterone production rate. Among the steroids tested, only 17α-methyl-testosterone and 17α-ethyl-19-nor-testosterone were effective in lowering significantly cortisone reduction rate by liver slices with concomitant decreases in microsomal Δ4-5α-hydrogenase-activity. Testosterone, 19-nor-testosterone, 17α-ethinyl-19-nor-testosterone, 17α-methyl-17β-hydroxy-androsta-1,4-dien-3-one and 1-methyl-17β-hydroxy-androst-1-en-3-one were ineffective or only slightly effective. Adrenal weight and absolute corticosterone production rate (μg/60 min per animal) were decreased after treatment with 17α-methyl-testosterone, 17α-ethyl-19-nor-testosterone and 1-methyl-17β-hydroxy-androst-1-en-3-one. Corticosterone production was decreased with 17α-ethinyl-19-nor-testosterone in spite of an unchanged adrenal weight. The relative corticosterone production rate (μg/60 min · 100 mg adrenal) was in any cases unaffected. According to these results there exists – with the exception of 17α-ethinyl-19-nor-testosterone – a strict parallelism between corticosteroid turnover and corticosterone production rate: unchanged turnover is correlated with unchanged corticosterone production rate, while a decreased turnover is correlated with decreased adrenal activity. The protein-anabolic effect of certain anabolic steroids may be partly due to an anti-catabolic action of these compounds resulting from a decreased corticosteroid inactivation and production rate. Possible mechanisms by which anabolic steroids may affect corticosteroid-balance are discussed.

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