Surface plasmon resonance biosensing based on target-responsive mobility switch of magnetic nanoparticles under magnetic fields

2011 ◽  
Vol 21 (13) ◽  
pp. 5156 ◽  
Author(s):  
Kyung Sig Lee ◽  
Mongryong Lee ◽  
Kyung Min Byun ◽  
In Su Lee
2012 ◽  
Vol 11 (1) ◽  
pp. 88-96 ◽  
Author(s):  
Tracy L. Kamikawa ◽  
Malgorzata G. Mikolajczyk ◽  
Michael Kennedy ◽  
Lilin Zhong ◽  
Pei Zhang ◽  
...  

Sensors ◽  
2022 ◽  
Vol 22 (2) ◽  
pp. 475
Author(s):  
Devendra Bhandari ◽  
Fur-Chi Chen ◽  
Roger C. Bridgman

Salmonella is one of the major foodborne pathogens responsible for many cases of illnesses, hospitalizations and deaths worldwide. Although different methods are available to timely detect Salmonella in foods, surface plasmon resonance (SPR) has the benefit of real-time detection with a high sensitivity and specificity. The purpose of this study was to develop an SPR method in conjunction with magnetic nanoparticles (MNPs) for the rapid detection of Salmonella Typhimurium. The assay utilizes a pair of well-characterized, flagellin-specific monoclonal antibodies; one is immobilized on the sensor surface and the other is coupled to the MNPs. Samples of romaine lettuce contaminated with Salmonella Typhimurium were washed with deionized water, and bacterial cells were captured on a filter membrane by vacuum filtration. SPR assays were compared in three different formats—direct assay, sequential two-step sandwich assay, and preincubation one-step sandwich assay. The interaction of flagellin and MNPs with the antibody-immobilized sensor surface were analyzed. SPR signals from a sequential two-step sandwich assay and preincubation one-step sandwich assay were 7.5 times and 14.0 times higher than the direct assay. The detection limits of the assay were 4.7 log cfu/mL in the buffer and 5.2 log cfu/g in romaine lettuce samples.


Materials ◽  
2021 ◽  
Vol 14 (12) ◽  
pp. 3354
Author(s):  
Changjin Son ◽  
Heongkyu Ju

We demonstrate magnetic control of optical reflectance with no ferromagnetic material via combining the Faraday rotation and the surface plasmon resonance (SPR) in a Kretschman configuration under magnetic fields < 0.5 T. The SPR produces the polarization sensitive reflectance from the Au or Ag thin film coated on a N-BK7 prism in which the Faraday rotation occurs. The gold (Au) or silver (Ag) metal film as a plasmonic film somewhat acts as an incident angle-dependent reflection polarizer that can sensitively sense the polarization change induced by the Faraday rotation that occurs in a prism. We find that combination of Faraday rotation and the surface plasmon can induce a significant magnetic modulation of reflectance normalized with respect to that obtained with no magnetic fields at a specific incident angle of light. The magnetic control of optical reflectance presented may find an application in polarizer-free photonic devices with no ferromagnetic material for magneto-optical modulation.


2020 ◽  
pp. 44-49
Author(s):  
I. N. Pavlov

Two optical methods, namely surface plasmon resonance imaging and frustrated total internal reflection, are described in the paper in terms of comparing their sensitivity to change of refractive index of a thin boundary layer of an investigated medium. It is shown that, despite the fact that the theoretically calculated sensitivity is higher for the frustrated total internal reflection method, and the fact that usually in practice the surface plasmon resonance method, on the contrary, is considered more sensitive, under the same experimental conditions both methods show a similar result.


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