Prevention of bacterial biofilms by covalent immobilization of peptides onto plasma polymer functionalized substrates

2010 ◽  
Vol 20 (37) ◽  
pp. 8092 ◽  
Author(s):  
Christelle Vreuls ◽  
Germaine Zocchi ◽  
Benjamin Thierry ◽  
Geoffrey Garitte ◽  
Stefani S. Griesser ◽  
...  
Keyword(s):  
Covalent Immobilization ◽  
Plasma Polymer ◽  
Bacterial Biofilms

Surface Immobilization of Synthetic Proteins Via Plasma Polymer Interlayers

1998 ◽  
Vol 544 ◽  
Author(s):  
Hans J Griesser ◽  
Keith M McLean ◽  
Gerrit J Beumer ◽  
Xiaoyi Gong ◽  
Peter Kingshot ◽  
...  
Keyword(s):  
Carboxylic Acid ◽  
Cell Attachment ◽  
Plasma Layer ◽  
Polymeric Materials ◽  
Covalent Immobilization ◽  
Biologically Active ◽  
Plasma Polymer ◽  
Rf Plasma ◽  
Bulk Materials ◽  
Synthetic Proteins

AbstractCoatings of biologically active molecules on synthetic ”bulk“materials are of much interest for biomedical applications since they can in principle elicit specific, predictable. controlled responses of the host environment to an implanted device. However, issues such as shelf life. storage conditions, biological safety, and enzymatic attack in the biological environment must be considered; synthetic proteins may offer advantages. In this study we investigated the covalent immobilization onto polymeric materials of synthetic proteins which possess some properties that mimic those of the natural protein collagen, particularly the ability to form triple helical structures, and thus may provide similar bio-responses while avoiding enzymatic degradation. In order to perform immobilization of these collagen-like molecules (CLMs) under mild reaction conditions, the bulk materials are first equipped with suitable surface groups using rf plasma methods. Plasma polymer interlayers offer advantages as versatile reactive platforms for the immobilization of proteins and other biologically active molecules. Application of a thin plasma polymer coating from an aldehyde monomer is particularly suitable as it enables direct immobilization of CLMs by reaction with their terminal amine groups, using reductive amination chemistry. An alternative route is via plasma polymer layers that contain carboxylic acid groups and using carbodiimnide chemistry. A third route makes use of alkylamme plasma polymer interlayers, which are less process sensitive than aldehyde and acid plasma coatings. A layer of poly-carboxylic acid compounds such as carboxylic acid terminated PAMAM-starburst dendrimers or carboxymethylated dextran is then attached by carbodiimide chemistry onto the amine plasma layer. Amine-terminated CLMs can then be immobilized onto the poly-carboxylic acid layer. Surface analytical methods have been used to characterize the immobilization steps and to assess the surface coverage. Initial cell attachment and growth assays indicate that the biological performance of the CLMs depends on their amino acid sequence.

Direct covalent attachment of silver nanoparticles on radical-rich plasma polymer films for antibacterial applications

2018 ◽  
Vol 6 (37) ◽  
pp. 5845-5853 ◽  
Author(s):  
Behnam Akhavan ◽  
Sadra Bakhshandeh ◽  
Hamed Najafi-Ashtiani ◽  
Ad C. Fluit ◽  
Edwin Boel ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Polymer Films ◽  
Plasma Polymerization ◽  
Covalent Immobilization ◽  
Single Step ◽  
Plasma Polymer ◽  
Covalent Attachment

Ion-assisted plasma polymerization for single-step, covalent immobilization of silver nanoparticles on surfaces.

Lipids and Free Fatty Acids of Red Sea Avrainvillea amadelpha, Holothuria atra, and Sarcocornia fruticosa Inhibit Marine Bacterial Biofilms

2020 ◽  
Vol 17 (6) ◽  
pp. 466-471
Author(s):  
Usama W. Hawas ◽  
Fekri Shaher ◽  
Mohamed Ghandourah ◽  
Lamia T. Abou El-Kassem ◽  
Sathianeson Satheesh ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Free Fatty Acids ◽  
Red Sea ◽  
Inhibitory Activity ◽  
Sea Cucumber ◽  
Bacterial Biofilms ◽  
Antibiofilm Activity ◽  
Bacterial Strains ◽  
Holothuria Atra ◽  
Sarcocornia Fruticosa

This study aimed at evaluating the antibiofilm activity of the Red Sea metabolites from green alga Avrainvillea amadelpha, sea cucumber Holothuria atra and costal plant Sarcocornia fruticosa against three biofilm bacterial strains isolated from Jeddah coast. Free fatty acids (FFAs) and other lipoidal matters were extracted from these organisms and analyzed by GC-MS. The composition of lipoidal fractions showed that A. amadelpha is rich by 74% saturated FAs, while sea cucumber H. atra revealed high content (60%) of unsaturated FAs. Palmitic acid is the major FA component in all species ranging from 14.5 to 26.7%. Phytol, sterols and hydrocarbons (C8-C29) were represented in the alga A. amadelpha as high contents with values 25.8, 21.9 and 18.5%, respectively. The extracts and lipoidal contents showed biofilm inhibitory activity against the isolated bacterial strains, where the unsaponified lipoidal fraction of S. fruticosa exhibited highest inhibitory activity against Planomicrobium sp. at concentration of 200 µg/mL.

GMP-Like Benzimidazole Broadly Inhibits Bacterial Biofilms

2011 ◽  
Vol 6 (11) ◽  
pp. 470-471
Author(s):  
David C. Holzman
Keyword(s):  
Bacterial Biofilms

The influence of fluid shear on the structure and material properties of sulphate-reducing bacterial biofilms

2002 ◽  
Vol 29 (6) ◽  
pp. 347-353 ◽  
Author(s):  
B C Dunsmore ◽  
A Jacobsen ◽  
L Hall-Stoodley ◽  
C J Bass ◽  
H M Lappin-Scott ◽  
...  
Keyword(s):  
Material Properties ◽  
Bacterial Biofilms ◽  
Fluid Shear

scholarly journals Pseudomonas aeruginosa PA14 Enhances the Efficacy of Norfloxacin against Staphylococcus aureus Newman Biofilms

2020 ◽  
Vol 202 (18) ◽  
Author(s):  
Giulia Orazi ◽  
Fabrice Jean-Pierre ◽  
George A. O’Toole
Keyword(s):  
Cystic Fibrosis ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Agents ◽  
Respiratory Infections ◽  
Multiple Infection ◽  
Bacterial Biofilms ◽  
Interspecies Interactions ◽  
Chronic Infections ◽  
Content Type

ABSTRACT The thick mucus within the airways of individuals with cystic fibrosis (CF) promotes frequent respiratory infections that are often polymicrobial. Pseudomonas aeruginosa and Staphylococcus aureus are two of the most prevalent pathogens that cause CF pulmonary infections, and both are among the most common etiologic agents of chronic wound infections. Furthermore, the ability of P. aeruginosa and S. aureus to form biofilms promotes the establishment of chronic infections that are often difficult to eradicate using antimicrobial agents. In this study, we found that multiple LasR-regulated exoproducts of P. aeruginosa, including 2-heptyl-4-hydroxyquinoline N-oxide (HQNO), siderophores, phenazines, and rhamnolipids, likely contribute to the ability of P. aeruginosa PA14 to shift S. aureus Newman norfloxacin susceptibility profiles. Here, we observe that exposure to P. aeruginosa exoproducts leads to an increase in intracellular norfloxacin accumulation by S. aureus. We previously showed that P. aeruginosa supernatant dissipates the S. aureus membrane potential, and furthermore, depletion of the S. aureus proton motive force recapitulates the effect of the P. aeruginosa PA14 supernatant on shifting norfloxacin sensitivity profiles of biofilm-grown S. aureus Newman. From these results, we hypothesize that exposure to P. aeruginosa PA14 exoproducts leads to increased uptake of the drug and/or an impaired ability of S. aureus Newman to efflux norfloxacin. Surprisingly, the effect observed here of P. aeruginosa PA14 exoproducts on S. aureus Newman susceptibility to norfloxacin seemed to be specific to these strains and this antibiotic. Our results illustrate that microbially derived products can alter the ability of antimicrobial agents to kill bacterial biofilms. IMPORTANCE Pseudomonas aeruginosa and Staphylococcus aureus are frequently coisolated from multiple infection sites, including the lungs of individuals with cystic fibrosis (CF) and nonhealing diabetic foot ulcers. Coinfection with P. aeruginosa and S. aureus has been shown to produce worse outcomes compared to infection with either organism alone. Furthermore, the ability of these pathogens to form biofilms enables them to cause persistent infection and withstand antimicrobial therapy. In this study, we found that P. aeruginosa-secreted products dramatically increase the ability of the antibiotic norfloxacin to kill S. aureus biofilms. Understanding how interspecies interactions alter the antibiotic susceptibility of bacterial biofilms may inform treatment decisions and inspire the development of new therapeutic strategies.

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