Gold nanoparticles: dispersibility in biological media and cell-biological effect

2010 ◽  
Vol 20 (29) ◽  
pp. 6176 ◽  
Author(s):  
Dirk Mahl ◽  
Christina Greulich ◽  
Wolfgang Meyer-Zaika ◽  
Manfred Köller ◽  
Matthias Epple
Keyword(s):  
Gold Nanoparticles ◽  
Biological Effect ◽  
Biological Media

scholarly journals Gold Nanostars with Reduced Fouling Facilitate Small Molecule Detection in the Presence of Protein

Nanomaterials ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 2565
Author(s):  
Anastasiia Tukova ◽  
Inga Christine Kuschnerus ◽  
Alfonso Garcia-Bennett ◽  
Yuling Wang ◽  
Alison Rodger
Keyword(s):  
Gold Nanoparticles ◽  
Small Molecule ◽  
Protein Corona ◽  
Surface Enhanced Raman Spectroscopy ◽  
Biological Media ◽  
Gold Nanostars ◽  
Cellular Environment ◽  
Vis Spectroscopy ◽  
Corona Formation

Gold nanoparticles have the potential to be used in biomedical applications from diagnostics to drug delivery. However, interactions of gold nanoparticles with different biomolecules in the cellular environment result in the formation of a “protein corona”—a layer of protein formed around a nanoparticle, which induces changes in the properties of nanoparticles. In this work we developed methods to reproducibly synthesize spheroidal and star-shaped gold nanoparticles, and carried out a physico-chemical characterization of synthesized anionic gold nanospheroids and gold nanostars through transmission electron microscopy (TEM), dynamic light scattering (DLS), zeta potential (ZP), nanoparticles tracking analysis (NTA), ultraviolet-visible (UV–Vis) spectroscopy and estimates of surface-enhanced Raman spectroscopy (SERS) signal enhancement ability. We analyzed how they interact with proteins after pre-incubation with bovine serum albumin (BSA) via UV–Vis, DLS, ZP, NTA, SERS, cryogenic TEM (cryo-TEM) and circular dichroism (CD) spectroscopy. The tests demonstrated that the protein adsorption on the particles’ surfaces was different for spheroidal and star shaped particles. In our experiments, star shaped particles limited the protein corona formation at SERS “hot spots”. This benefits the small-molecule sensing of nanostars in biological media. This work adds more understanding about protein corona formation on gold nanoparticles of different shapes in biological media, and therefore guides design of particles for studies in vitro and in vivo.

scholarly journals Synthesis of Gold Functionalised Nanoparticles with the Eranthis hyemalis Lectin and Preliminary Toxicological Studies on Caenorhabditis elegans

2018 ◽  
Author(s):  
Jamila Djafari ◽  
Marie T. McConnell ◽  
Hugo M. Santos ◽  
José Luis Capelo ◽  
Emilia Bertolo ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Caenorhabditis Elegans ◽  
Biological Effect ◽  
Life Stage ◽  
Target Cells ◽  
Type Ii ◽  
Biological Assays ◽  
C Elegans ◽  
Toxicological Studies ◽  
Vis Spectroscopy

The lectin found in the tubers of the Winter Aconite (Eranthis hyemalis) plant (EHL) is a Type II Ribosome Inactivating Protein (RIP); type II RIPs have shown anti-cancer properties, and have great potential as therapeutic agents. Similarly, colloidal gold nanoparticles are successfully used in biomedical applications as gold nanoparticles can be functionalised with ligands with high affinity and specificity for target cells to create therapeutic and imaging agents. Herein we present the synthesis and characterization of gold nanoparticles conjugated with EHL. The aim was to establish the viability of the conjugate and perform a set of initial assays to establish whether the biological effect of EHL is altered by the conjugation. The biological assays were performed in Caenorhabditis elegans, a free living nematode commonly used for toxicological studies; previous work from some of the authors using first life stage (L1) nematodes has shown that EHL has a strong biocidal effect on C. elegans. Gold nanoparticles functionalised with EHL (AuNPs@EHL) were successfully synthesised by bioconjugation with citrate gold nanoparticles (AuNPs@Citrate); the conjugates were analysed by UV-Vis spectroscopy, Dynamic Light Scattering (DLS), Zeta Potential analysis and Transmission Electron Microscopy (TEM). Results indicate that an optimal functionalisation was achieved with the addition of 100 µL of EHL (concentration 1090 ± 40 µg/mL) over 5 mL of AuNPs (concentration [Au0] = 0.8 mM). Biological assays on the effect of AuNPs@EHL on C. elegans were performed, using first life stage (L1) and pre-adult stage (L4) nematodes. Citrate gold nanoparticles did not have any obvious effect on the nematodes. For L1 stage nematodes, the assays show that conjugation with gold nanoparticles reduced the biological effect of EHL on C. elegans. As lectin binding activity is essential for the natural protein to bind and allow entry to cells, conformational changes due to conjugation may have affected this binding affinity. For L4 stage nematodes, both EHL alone and AuNPs@EHL showed biological activity, and reproductive delays and reduced fecundity were observed in both cases. These assays indicate that EHL can be conjugated to gold nanoparticles and retain elements of biocidal activity.

Negatively charged gold nanoparticles as a dexamethasone carrier: stability in biological media and bioactivity assessment in vitro

RSC Advances ◽  
2016 ◽  
Vol 6 (101) ◽  
pp. 99016-99022 ◽  
Author(s):  
A. Rossi ◽  
S. Donati ◽  
L. Fontana ◽  
F. Porcaro ◽  
C. Battocchio ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Drug Release ◽  
Release Kinetics ◽  
Biological Media ◽  
Drug Release Kinetics

AuNP and AuNP/DXM stability and drug release kinetics in different biological media.

scholarly journals N-Heterocyclic Carbene Ligand Stability on Gold Nanoparticles in Biological Media

ACS Omega ◽  
2021 ◽  
Author(s):  
Lindy M. Sherman ◽  
Matthew D. Finley ◽  
Rowan K. Borsari ◽  
Naviya Schuster-Little ◽  
Shelby L. Strausser ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Biological Media

scholarly journals Synthesis of Gold Functionalised Nanoparticles with the Eranthis hyemalis Lectin and Preliminary Toxicological Studies on Caenorhabditis elegans

Materials ◽  
2018 ◽  
Vol 11 (8) ◽  
pp. 1363 ◽  
Author(s):  
Jamila Djafari ◽  
Marie T. McConnell ◽  
Hugo M. Santos ◽  
José Luis Capelo ◽  
Emilia Bertolo ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Caenorhabditis Elegans ◽  
Biological Effect ◽  
Target Cells ◽  
Type Ii ◽  
Obvious Effect ◽  
Transmission Electron ◽  
Toxicological Studies ◽  
Vis Spectroscopy ◽  
Free Living Nematode

The lectin found in the tubers of the Winter Aconite (Eranthis hyemalis) plant (EHL) is a Type II Ribosome Inactivating Protein (RIP). Type II RIPs have shown anti-cancer properties and have great potential as therapeutic agents. Similarly, colloidal gold nanoparticles are successfully used in biomedical applications as they can be functionalised with ligands with high affinity and specificity for target cells to create therapeutic and imaging agents. Here we present the synthesis and characterization of gold nanoparticles conjugated with EHL and the results of a set of initial assays to establish whether the biological effect of EHL is altered by the conjugation. Gold nanoparticles functionalised with EHL (AuNPs@EHL) were successfully synthesised by bioconjugation with citrate gold nanoparticles (AuNPs@Citrate). The conjugates were analysed by UV-Vis spectroscopy, Dynamic Light Scattering (DLS), Zeta Potential analysis, and Transmission Electron Microscopy (TEM). Results indicate that an optimal functionalisation was achieved with the addition of 100 µL of EHL (concentration 1090 ± 40 µg/mL) over 5 mL of AuNPs (concentration [Au0] = 0.8 mM). Biological assays on the effect of AuNPs@EHL were undertaken on Caenorhabditis elegans, a free-living nematode commonly used for toxicological studies, that has previously been shown to be strongly affected by EHL. Citrate gold nanoparticles did not have any obvious effect on the nematodes. For first larval stage (L1) nematodes, AuNPs@EHL showed a lower biological effect than EHL. For L4 stage, pre-adult nematodes, both EHL alone and AuNPs@EHL delayed the onset of reproduction and reduced fecundity. These assays indicate that EHL can be conjugated to gold nanoparticles and retain elements of biocidal activity.

Non-covalent binding of nucleic acids with gold nanoparticles provides their stability and effective desorption in environment mimicking biological media

2018 ◽  
Vol 29 (35) ◽  
pp. 355601 ◽  
Author(s):  
Anna Epanchintseva ◽  
Anton Dolodoev ◽  
Alina Grigor’eva ◽  
Boris Chelobanov ◽  
Dmitrii Pyshnyi ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Nucleic Acids ◽  
Covalent Binding ◽  
Biological Media
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