Microfluidic devices for in vitro studies on liver drug metabolism and toxicity

2011 ◽  
Vol 3 (5) ◽  
pp. 509 ◽  
Author(s):  
Paul M. van Midwoud ◽  
Elisabeth Verpoorte ◽  
Geny M. M. Groothuis
Keyword(s):  
Drug Metabolism ◽  
Microfluidic Devices ◽  
In Vitro Studies

scholarly journals The Effect of Microfluidic Geometry on Myoblast Migration

Micromachines ◽  
2019 ◽  
Vol 10 (2) ◽  
pp. 143
Author(s):  
Rahul Atmaramani ◽  
Bryan Black ◽  
Kevin Lam ◽  
Vinit Sheth ◽  
Joseph Pancrazio ◽  
...  
Keyword(s):  
Cell Migration ◽  
Microfluidic Devices ◽  
In Vitro Studies ◽  
Future Design ◽  
The Future ◽  
Pdms Microchannel ◽  
In Vitro Systems ◽  
Spontaneous Migration ◽  
Over Time

In vitro systems comprised of wells interconnected by microchannels have emerged as a platform for the study of cell migration or multicellular models. In the present study, we systematically evaluated the effect of microchannel width on spontaneous myoblast migration across these microchannels—from the proximal to the distal chamber. Myoblast migration was examined in microfluidic devices with varying microchannel widths of 1.5–20 µm, and in chips with uniform microchannel widths over time spans that are relevant for myoblast-to-myofiber differentiation in vitro. We found that the likelihood of spontaneous myoblast migration was microchannel width dependent and that a width of 3 µm was necessary to limit spontaneous migration below 5% of cells in the seeded well after 48 h. These results inform the future design of Polydimethylsiloxane (PDMS) microchannel-based co-culture platforms as well as future in vitro studies of myoblast migration.

The influence of age on drug metabolism: in vivo and in vitro studies

1990 ◽  
Vol 183 (5) ◽  
pp. 1629-1630 ◽  
Author(s):  
K.F.A. van Bezooijen ◽  
K. Groen ◽  
D.D. Breimer
Keyword(s):  
Drug Metabolism ◽  
In Vitro Studies

In Vitro Studies of Drug Metabolism

2007 ◽  
pp. 231-257 ◽  
Author(s):  
Y. Parmentier ◽  
M.-J. Bossant ◽  
M. Bertrand ◽  
B. Walther
Keyword(s):  
Drug Metabolism ◽  
In Vitro Studies

Influence of liver size on the relationship between in vivo and in vitro studies of drug metabolism

1978 ◽  
Vol 3 (4) ◽  
pp. 217-222 ◽  
Author(s):  
Heikki I. Pirttiaho ◽  
Eero A. Sotaniemi ◽  
R. Olavi Pelkonen ◽  
Uolevi Pitkänen
Keyword(s):  
Drug Metabolism ◽  
In Vitro Studies ◽  
Liver Size ◽  
The Relationship

In vitro - studies with antler bone cells: Structure forming capacity, osteocalcin production and influence of sex steroids

2006 ◽  
Vol 15 (04) ◽  
pp. 245-257 ◽  
Author(s):  
H. J. Rolf ◽  
K. G. Wiese ◽  
H. Siggelkow ◽  
H. Schliephake ◽  
G. A. Bubernik
Keyword(s):  
Sex Steroids ◽  
Bone Cells ◽  
In Vitro Studies ◽  
Osteocalcin Production

In Vitro Studies on the Mechanism of the Antifibrino-lytic Action of E-Aminocaproic Acid (EACA)

1968 ◽  
Vol 19 (03/04) ◽  
pp. 584-592 ◽  
Author(s):  
Hanna Lukasiewicz ◽  
S Niewiarowski
Keyword(s):  
Aminocaproic Acid ◽  
Test Tube ◽  
In Vitro Studies ◽  
Lytic Action ◽  
Human Plasminogen ◽  
Experimental Findings ◽  
Fibrin Clots

Summary and Conclusion1. It has been found that EACA does not inhibit activation of human plasminogen into plasmin by SK and UK in a concentration of 5 × 10–2 M. The activation of bovine plasminogen by SK and UK is inhibited by this concentration of EACA but not by a lower one.2. EACA in concentrations of 1,5 × 10–1 – 10–4 M does not inhibit casein proteolysis by plasmin. The proteolysis of fibrinogen and fibrin measured by the release of TCA soluble tyrosine is inhibited by EACA in concentrations of 1,5 × 10–1 – 10–2 M.3. The lysis of non-stabilized clots by plasmin measured in a test tube was inhibited by an EACA concentration of 5 × 10–3 – 5 × 10–4 M. The lysis of stabilized clots by plasmin was inhibited by an EACA concentration of 10–5 M.4. On the basis of experimental findings and data given in literature the authors postulate that the mechanism of the antifibrinolytic effects of EACA consists mainly in a modification of plasmin action on fibrin. These effects are dependent on the structure of the fibrin clots.

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