Adhesive properties of Staphylococcus epidermidis probed by atomic force microscopy

2011 ◽  
Vol 13 (21) ◽  
pp. 9995 ◽  
Author(s):  
Yifan Hu ◽  
Jens Ulstrup ◽  
Jingdong Zhang ◽  
Søren Molin ◽  
Vincent Dupres
Keyword(s):  
Atomic Force Microscopy ◽  
Staphylococcus Epidermidis ◽  
Adhesive Properties ◽  
Force Microscopy ◽  
Atomic Force

scholarly journals Atomic force microscopy of a ctpA mutant in Rhizobium leguminosarum reveals surface defects linking CtpA function to biofilm formation

Microbiology ◽  
2011 ◽  
Vol 157 (11) ◽  
pp. 3049-3058 ◽  
Author(s):  
Jun Dong ◽  
Karla S. L. Signo ◽  
Elizabeth M. Vanderlinde ◽  
Christopher K. Yost ◽  
Tanya E. S. Dahms
Keyword(s):  
Atomic Force Microscopy ◽  
Mutant Strain ◽  
Biofilm Formation ◽  
Rhizobium Leguminosarum ◽  
Surface Defects ◽  
Ion Concentration ◽  
Adhesive Properties ◽  
Surface Ultrastructure ◽  
Force Microscopy ◽  
Atomic Force

Atomic force microscopy was used to investigate the surface ultrastructure, adhesive properties and biofilm formation of Rhizobium leguminosarum and a ctpA mutant strain. The surface ultrastructure of wild-type R. leguminosarum consists of tightly packed surface subunits, whereas the ctpA mutant has much larger subunits with loose lateral packing. The ctpA mutant strain is not capable of developing fully mature biofilms, consistent with its altered surface ultrastructure, greater roughness and stronger adhesion to hydrophilic surfaces. For both strains, surface roughness and adhesive forces increased as a function of calcium ion concentration, and for each, biofilms were thicker at higher calcium concentrations.

Effect of ampicillin on adhesive properties of bacteria examined by atomic force microscopy

Micron ◽  
2018 ◽  
Vol 112 ◽  
pp. 84-90 ◽  
Author(s):  
Dariusz Laskowski ◽  
Janusz Strzelecki ◽  
Konrad Pawlak ◽  
Hanna Dahm ◽  
Aleksander Balter
Keyword(s):  
Atomic Force Microscopy ◽  
Adhesive Properties ◽  
Force Microscopy ◽  
Atomic Force

scholarly journals Atomic Force Microscopy of Cell Growth and Division in Staphylococcus aureus

2004 ◽  
Vol 186 (11) ◽  
pp. 3286-3295 ◽  
Author(s):  
Ahmed Touhami ◽  
Manfred H. Jericho ◽  
Terry J. Beveridge
Keyword(s):  
Staphylococcus Aureus ◽  
Atomic Force Microscopy ◽  
Cell Wall ◽  
Cross Wall ◽  
Adhesive Properties ◽  
Reactive Material ◽  
Force Microscopy ◽  
Additional Information ◽  
Atomic Force ◽  
Sequential Images

ABSTRACT The growth and division of Staphylococcus aureus was monitored by atomic force microscopy (AFM) and thin-section transmission electron microscopy (TEM). A good correlation of the structural events of division was found using the two microscopies, and AFM was able to provide new additional information. AFM was performed under water, ensuring that all structures were in the hydrated condition. Sequential images on the same structure revealed progressive changes to surfaces, suggesting the cells were growing while images were being taken. Using AFM small depressions were seen around the septal annulus at the onset of division that could be attributed to so-called murosomes (Giesbrecht et al., Arch. Microbiol. 141:315-324, 1985). The new cell wall formed from the cross wall (i.e., completed septum) after cell separation and possessed concentric surface rings and a central depression; these structures could be correlated to a midline of reactive material in the developing septum that was seen by TEM. The older wall, that which was not derived from a newly formed cross wall, was partitioned into two different surface zones, smooth and gel-like zones, with different adhesive properties that could be attributed to cell wall turnover. The new and old wall topographies are equated to possible peptidoglycan arrangements, but no conclusion can be made regarding the planar or scaffolding models.

scholarly journals Assessment of Elasticity and Topography of Aspergillus nidulans Spores via Atomic Force Microscopy

2005 ◽  
Vol 71 (2) ◽  
pp. 955-960 ◽  
Author(s):  
Liming Zhao ◽  
David Schaefer ◽  
Mark R. Marten
Keyword(s):  
Atomic Force Microscopy ◽  
Aspergillus Nidulans ◽  
Fungal Spores ◽  
Protein Structures ◽  
Spore Surface ◽  
Wild Type ◽  
Adhesive Properties ◽  
Force Microscopy ◽  
Atomic Force ◽  
Tapping Mode Afm

ABSTRACT Previous studies have described both surface morphology and adhesive properties of fungal spores, but little information is currently available on their mechanical properties. In this study, atomic force microscopy (AFM) was used to investigate both surface topography and micromechanical properties of Aspergillus nidulans spores. To assess the influence of proteins covering the spore surface, wild-type spores were compared with spores from isogenic rodA + and rodA − strains. Tapping-mode AFM images of wild-type and rodA + spores in air showed characteristic “rodlet” protein structures covering a granular spore surface. In comparison, rodA − spores were rodlet free but showed a granular surface structure similar to that of the wild-type and rodA + spores. Rodlets were removed from rodA + spores by sonication, uncovering the underlying granular layer. Both rodlet-covered and rodlet-free spores were subjected to nanoindentation measurements, conducted in air, which showed the stiffnesses to be 110 ± 10, 120 ± 10, and 300 ± 20 N/m and the elastic moduli to be 6.6 ± 0.4, 7.0 ± 0.7, and 22 ± 2 GPa for wild-type, rodA + and rodA − spores, respectively. These results imply the rodlet layer is significantly softer than the underlying portion of the cell wall.

scholarly journals Atomic force microscopy-based microrheology reveals significant differences in the viscoelastic response between malign and benign cell lines

Open Biology ◽  
2014 ◽  
Vol 4 (5) ◽  
pp. 140046 ◽  
Author(s):  
Jan Rother ◽  
Helen Nöding ◽  
Ingo Mey ◽  
Andreas Janshoff
Keyword(s):  
Mechanical Properties ◽  
Atomic Force Microscopy ◽  
Cell Lines ◽  
Loss Tangent ◽  
Structural Changes ◽  
Metastatic Potential ◽  
Adhesive Properties ◽  
Force Microscopy ◽  
Atomic Force ◽  
Independent Information

Mechanical phenotyping of cells by atomic force microscopy (AFM) was proposed as a novel tool in cancer cell research as cancer cells undergo massive structural changes, comprising remodelling of the cytoskeleton and changes of their adhesive properties. In this work, we focused on the mechanical properties of human breast cell lines with different metastatic potential by AFM-based microrheology experiments. Using this technique, we are not only able to quantify the mechanical properties of living cells in the context of malignancy, but we also obtain a descriptor, namely the loss tangent, which provides model-independent information about the metastatic potential of the cell line. Including also other cell lines from different organs shows that the loss tangent ( G″ / G′ ) increases generally with the metastatic potential from MCF-10A representing benign cells to highly malignant MDA-MB-231 cells.

scholarly journals Competition of elastic and adhesive properties of carbon nanotubes anchored to atomic force microscopy tips

2007 ◽  
Vol 19 (3) ◽  
pp. 035709 ◽  
Author(s):  
Charlotte Bernard ◽  
Sophie Marsaudon ◽  
Rodolphe Boisgard ◽  
Jean-Pierre Aimé
Keyword(s):  
Carbon Nanotubes ◽  
Atomic Force Microscopy ◽  
Adhesive Properties ◽  
Force Microscopy ◽  
Atomic Force

Simultaneous Analysis of Elastic and Nonspecific Adhesive Properties of Thin Sample and Biological Cell Considering Bottom Substrate Effect

2017 ◽  
Vol 139 (9) ◽  
Author(s):  
Vishwanath Managuli ◽  
Sitikantha Roy
Keyword(s):  
Atomic Force Microscopy ◽  
Correction Term ◽  
Work Of Adhesion ◽  
Common Source ◽  
Substrate Effect ◽  
Adhesive Properties ◽  
Adhesion Properties ◽  
Force Microscopy ◽  
Bottom Substrate ◽  
Atomic Force

A new asymptotically correct contact model has been developed for conical tip based atomic force microscopy (AFM) nanoindentation. This new model provides both elastic and nonspecific adhesion properties of cells and soft gels by taking sample thickness at the point of indentation and its depth of indentation into consideration. The bottom substrate effect (BSE) is the most common source of error in the study of “AFM force maps” of the cellular sample. The present model incorporates an asymptotically correct correction term as a function of depth of indentation to eliminate the substrate effect in the analysis. Later, the model is extended to analyze the unloading portion of the indentation curve to extract the stiffness and adhesive properties simultaneously. A comparative study of the estimated material properties using other established contact models shows that the provided corrections effectively curb the errors coming from infinite thickness assumption. Nonspecific adhesive nature of a cell is represented in terms of adhesion parameter (γa) based on the “work of adhesion,” this is an alternative to the peak value of tip–sample attractive (negative) force commonly used as representative adhesion measurement. The simple analytical expression of the model can help in estimating more realistic and accurate biomechanical properties of cells from atomic force microscopy based indentation technique.

Atomic force microscopy identifies regions of distinct desmoglein 3 adhesive properties on living keratinocytes

2015 ◽  
Vol 11 (3) ◽  
pp. 511-520 ◽  
Author(s):  
Franziska Vielmuth ◽  
Eva Hartlieb ◽  
Daniela Kugelmann ◽  
Jens Waschke ◽  
Volker Spindler
Keyword(s):  
Atomic Force Microscopy ◽  
Adhesive Properties ◽  
Force Microscopy ◽  
Atomic Force ◽  
Desmoglein 3

scholarly journals Characteristic of adhesive properties of endothelium and subendothelial structures of aortic vlve in patients with calcific aortic stenosis

2017 ◽  
Vol 95 (4) ◽  
pp. 350-354
Author(s):  
N. I. Gulyaev ◽  
M. V. Zhukov ◽  
A. O. Golubok ◽  
G. L. Kuranov ◽  
Yu. A. Borisov ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Chemical Composition ◽  
Aortic Stenosis ◽  
Ectopic Calcification ◽  
Adhesive Properties ◽  
Calcific Aortic Stenosis ◽  
Calcium Salts ◽  
Force Microscopy ◽  
Atomic Force

The article presents results of research on adhesive properties of aortic crescents (endothelial and subendothelial surface) obtained with the use of atomic force microscopy and specially created hydroxyapatite-based probes along with results of identification of the chemical composition of deposited calcium salts in patients with calcified aortic stenosis. The results of the study are supposed to be of pathogenetic significance for understanding the development of calcified aortic stenosis. The proposed method for creating specialized probes for atomic force microscopy can be used in the modeling of processes of ectopic calcification of other organs and system.

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