Improved electrochemical competitive immunosensor for ochratoxin A with a biotinylated monoclonal antibody capture probe and colloidal gold nanostructuring

2011 ◽  
Vol 3 (4) ◽  
pp. 977 ◽  
Author(s):  
Juan C. Vidal ◽  
Laura Bonel ◽  
Patricia Duato ◽  
Juan R. Castillo
Keyword(s):  
Monoclonal Antibody ◽  
Ochratoxin A ◽  
Colloidal Gold ◽  
Capture Probe ◽  
Competitive Immunosensor ◽  
Antibody Capture

Identification of the pulmonary syndrome hantavirus by direct and colloidal gold immune Electron Microscopy

1994 ◽  
Vol 52 ◽  
pp. 274-275
Author(s):  
C. D. Humphrey ◽  
C.S. Goldsmith ◽  
L. Elliott ◽  
S.R. Zaki
Keyword(s):  
Electron Microscopy ◽  
United States ◽  
Monoclonal Antibody ◽  
Colloidal Gold ◽  
Phosphotungstic Acid ◽  
Uranyl Acetate ◽  
Hantavirus Pulmonary Syndrome ◽  
Deer Mice ◽  
Immune Electron Microscopy ◽  
Negative Stain

An outbreak of unexplained acute pulmonary syndrome with high fatality was recognized in the spring of 1993 in the southwestern United States. The cause of the illness was quickly identified serologically and genetically as a hantavirus and the disease was named hantavirus pulmonary syndrome (HPS). Recently, the virus was isolated from deer mice which had been trapped near the homes of HPS patients, and cultivated in Vero E6 cells. We identified the cultivated virus by negative-stain direct and colloidal gold immune electron microscopy (EM).Virus was extracted, clarified, and concentrated from unfixed and 0.25% glutaraldehyde fixed supernatant fluids of infected Vero E6 cells by a procedure described previously. Concentrated virus suspensions tested by direct EM were applied to glow-discharge treated formvar-carbon filmed grids, blotted, and stained with 0.5% uranyl acetate (UA) or with 2% phosphotungstic acid (PTA) pH 6.5. Virus suspensions for immune colloidal gold identification were adsorbed similarly to filmed grids but incubated for 1 hr on drops of 1:50 diluted monoclonal antibody to Prospect Hill virus nucleoprotein or with 1:50 diluted sera from HPS virus infected deer mice.

scholarly journals A comparison between enzyme immunoassay and HPLC for ochratoxin A detection in green, roasted and instant coffee

2007 ◽  
Vol 50 (2) ◽  
pp. 349-359 ◽  
Author(s):  
Simone Fujii ◽  
Elisabete Yurie Sataque Ono ◽  
Ricardo Marcelo Reche Ribeiro ◽  
Fernanda Garcia Algarte Assunção ◽  
Cássia Reika Takabayashi ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Ochratoxin A ◽  
High Performance ◽  
Screening Tool ◽  
Correlation Coefficients ◽  
Enzyme Linked Immunosorbent Assay ◽  
Elisa Assay ◽  
Quality And Safety ◽  
Green Coffee ◽  
Instant Coffee

An indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) for ochratoxin A (OTA) detection in green, roasted and instant coffees was developed using anti-OTA monoclonal antibody. Immunological reagents prepared were OTA-BSA (4.76 mg/mL), anti-OTA.7 MAb (2x10³-fold dilution) and HRP-anti IgG (10³-fold dilution). The detection limit was 3.73 ng OTA/g and correlation coefficients (r) between this immunoassay and high performance liquid chromatography were 0.98 for green coffee, 0.98 for roasted and 0.86 for instant. OTA levels detected by ic-ELISA were higher than by HPLC, with ELISA/HPLC ratio of 0.66 - 1.46 (green coffee), 0.96 - 1.11 (roasted) and 0.93 - 1.82 (instant). ELISA recoveries for OTA added to coffee (5 - 70 ng/g) were 81.53 % for green coffee, 46.73 % for roasted and 64.35 % for instant, while recoveries by HPLC were 80.54 %, 45.91 % and 55.15 %, respectively. Matrices interferences were minimized by samples dilution before carrying out the ELISA assay. The results indicate that MAb-based ic-ELISA could be a simple, sensitive and specific screening tool for OTA detection, contributing to quality and safety of coffee products.

scholarly journals Production and characterization of monoclonal antibodies to the edta extract of Leptospira interrogans, serovar icterohaemorrhagiae

1996 ◽  
Vol 29 (5) ◽  
pp. 483-489
Author(s):  
Lilian Terezinha de Queiroz Leite ◽  
Mauricio Resende ◽  
Wanderley de Souza ◽  
Elizabeth R.S. Camargos ◽  
Matilde Cota Koury
Keyword(s):  
Monoclonal Antibody ◽  
Monoclonal Antibodies ◽  
Western Blot ◽  
Silver Staining ◽  
Colloidal Gold ◽  
Leptospira Interrogans ◽  
Outer Envelope ◽  
Lethal Challenge ◽  
Sds Page

Monoclonal antibodies (MABs) ivere produced against an etbylenediaminetetraacetate (EDTA) extract of Leptospira interrogans serovar icterohaemorrhagiae being characterized by gel precipitation as IgM and IgG (IgGl and IgG2b). The EDTA extract was detected as several bands by silver staining in SDS-PAGE. In the Western blot the bands around 20 KDa reacted with a monoclonal antibody, 47B4D6, and was oxidized by periodate and was not digested by pronase, suggesting that the determinant is of carbohydrate nature, lmmunocytochemistry, using colloidal gold labeling, showed that an EDTA extract determinant recognized by monoclonal antibody 47B4D6, is localized under the outer envelope of serovar icterohaemorrhagiae. Hoe AIAB raised against the EDTA extract was not able to protect hamsters from lethal challenge with virulent homologous leptospires.

Development of a colloidal gold strip for rapid detection of ochratoxin A with mimotope peptide

Food Control ◽  
2009 ◽  
Vol 20 (9) ◽  
pp. 791-795 ◽  
Author(s):  
Weihua Lai ◽  
Daniel Y.C. Fung ◽  
Xu Yang ◽  
Liu Renrong ◽  
Yonghua Xiong
Keyword(s):  
Ochratoxin A ◽  
Colloidal Gold ◽  
Rapid Detection

Colloidal Gold–Based Immunochromatographic Test Strip for Rapid Detection of Abrin in Food Samples

2012 ◽  
Vol 75 (1) ◽  
pp. 112-117 ◽  
Author(s):  
SHAN GAO ◽  
CHONG NIE ◽  
JUNHONG WANG ◽  
JING WANG ◽  
LIN KANG ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Polyclonal Antibody ◽  
Colloidal Gold ◽  
Test Strip ◽  
Food Samples ◽  
Visual Observation ◽  
Quantitative Detection ◽  
Capture Antibody ◽  
A Chain ◽  
Ic Test

In the present study, we developed a convenient, rapid, and sensitive immunochromatographic (IC) test strip to detect abrin in assay buffer and spiked abrin in test food samples. The abrin IC test strip was based on a sandwich format consisting of a monoclonal antibody and a polyclonal antibody. The anti-abrin A chain monoclonal antibody from mice was immobilized on a porous nitrocellulose membrane as a capture antibody, while the anti-abrin polyclonal antibody from rabbits was conjugated to colloidal gold particles, serving as a detection antibody. Both visual observation and quantitative analysis indicated that the lower detection of the strip was about 3 ng/ml when abrin was directly spiked into milk, orange juice, and drinking water at a concentration of 3 to 60 ng/ml; the analytical recovery rate was 92.2 to 128%. With this method, abrin spiked into food could be detected in less than 10 min. Moreover, the IC test strip showed no cross-reaction with the closely related phytotoxin ricin. Therefore, our test strip is an ideal candidate for the development of a kit for rapid and quantitative detection of abrin in food samples.

scholarly journals Measurement of MHC/Peptide Interactions by Gel Filtration or Monoclonal Antibody Capture

2013 ◽  
Vol 100 (1) ◽  
Author(s):  
John Sidney ◽  
Scott Southwood ◽  
Carrie Moore ◽  
Carla Oseroff ◽  
Clemencia Pinilla ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Gel Filtration ◽  
Peptide Interactions ◽  
Antibody Capture

Continuous precipitation for monoclonal antibody capture using countercurrent washing by microfiltration

2019 ◽  
Vol 35 (6) ◽  
Author(s):  
Zhao Li ◽  
Qin Gu ◽  
Jonathan L. Coffman ◽  
Todd Przybycien ◽  
Andrew L. Zydney
Keyword(s):  
Monoclonal Antibody ◽  
Continuous Precipitation ◽  
Antibody Capture

Monoclonal antibody capture and viral clearance by cation exchange chromatography

2012 ◽  
Vol 109 (8) ◽  
pp. 2048-2058 ◽  
Author(s):  
G.R. Miesegaes ◽  
S. Lute ◽  
D.M. Strauss ◽  
E.K. Read ◽  
A. Venkiteshwaran ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Cation Exchange ◽  
Exchange Chromatography ◽  
Viral Clearance ◽  
Cation Exchange Chromatography ◽  
Antibody Capture

Platelet release reaction: Visualization of secreted fibrinogen by the monoclonal antibody (D4) directly coupled to colloidal gold

1992 ◽  
Vol 65 ◽  
pp. S140
Author(s):  
J. Suttnar ◽  
N. Belitser ◽  
Y. Veklich ◽  
M. Anishuk ◽  
T. Pozdnyakova ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Colloidal Gold ◽  
Release Reaction ◽  
Platelet Release
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