scholarly journals Comparative analysis of protein extraction methods for the identification of seafood-borne pathogenic and spoilage bacteria by MALDI-TOF mass spectrometry

2010 ◽  
Vol 2 (12) ◽  
pp. 1941 ◽  
Author(s):  
Karola Böhme ◽  
Inmaculada C. Fernández-No ◽  
Jorge Barros-Velázquez ◽  
Jose M. Gallardo ◽  
Benito Cañas ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Comparative Analysis ◽  
Protein Extraction ◽  
Extraction Methods ◽  
Maldi Tof Mass Spectrometry ◽  
Maldi Tof ◽  
Spoilage Bacteria

scholarly journals NEW OPPORTUNITIES TO IDENTIFY AND TYPE STAPHYLOCOCCUS spp. BY USING MALDI-TOF MASS SPECTROMETRY

2019 ◽  
Vol 8 (4) ◽  
pp. 489-496
Author(s):  
A. S. Stepanov ◽  
N. V. Vasilyeva
Keyword(s):  
Mass Spectrometry ◽  
Sample Preparation ◽  
Protein Extraction ◽  
Mass Range ◽  
Intensity Level ◽  
Immunocompromised Patients ◽  
Microbial Identification ◽  
Maldi Tof Mass Spectrometry ◽  
Maldi Tof ◽  
Staphylococcus Spp

Abstract.Mass spectrometry profiles of microorganisms obtained by time-of-flight matrix-associated laser desorption/ionization (MALDI-TOF) mass spectrometry are a source of information about peptide profiles can be used for microbial identification and typing. A variety of technical and bioinformational solutions complicate developing of a united mass-spectro-profile database. Staphylococcus spp. strains are good studied objects for identification by MALDI-TOF mass spectrometry, frequently resulting in nosocomial infections, especially in immunocompromised patients. Rapid differentiation of nosocomial, multiresistant and highly virulent isolates of Staphylococcus spp. Allows to reduce the lethality in weakened and immunocompromised patients. The study was aimed at assessing comparability and reproducibility of identification and typing results for Staphylococcus spp by MALDI-TOF mass spectrometry. Comparing 292 Staphylococcus spp. isolates in clinical specimens obtained fron the multidisciplinary hospital at the NWSMU im. I.I. Mechnikov was carried out by using MALDI-TOF mass spectrometer BactoSCREEN ID (Litech, Russia) and Bruker Biotyper 3.1 (Bruker GmbH, Germany). Comparability of Staphylococcus spp. identification showed that 95.9%; 12 isolates (4.1%) by “Bruker Biotyper 3.1” and 3 isolates (1.1%) by using “BactoSCREEN ID” were incorrectly identified. Repeated identification leveled the differences between the systems used. In addition, it was shown that the method of protein extraction did not affect reliability of Staphylococcus spp. species identification by using databases (÷2, p > 0.05) compared to intraspecific typing (÷2, p < 0.0001). Using different extraction protocols showed that Staphylococcus spp. mass-spectra differed by peak intensity level within the mass range up to 4000 m/z, 5300±600 m/z and 6500±500 m/z, as well as higher than 7000 m/z. Peaks of low-molecular weight peptides were detected under full protein extraction compared to sample preparation on plate extraction. To develop a unified protocol for mass-spectrometry profile processing, a reliability of the basic statistical variables (mode, median, maximum, minimum and arithmetic mean) was evaluated. Analysis of the median mass spectrometry profiles is recommended for Staphylococcus spp. intraspecific typing by using MALDI-TOF mass spectrometry as the most reproducible and consistent approach. As a result, two systems for MALDI-TOF mass spectrometry reliably identify Staphylococcus spp., but standardization of sample preparation and bioinformation analysis is required for Staphylococcus spp. typing.

scholarly journals Rapid separation and identification of beer spoilage bacteria by inertial microfluidics and MALDI-TOF mass spectrometry

Lab on a Chip ◽  
2019 ◽  
Vol 19 (11) ◽  
pp. 1961-1970 ◽  
Author(s):  
Mark R. Condina ◽  
Brooke A. Dilmetz ◽  
Sajad Razavi Bazaz ◽  
Jon Meneses ◽  
Majid Ebrahimi Warkiani ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Throughput ◽  
Accurate Method ◽  
Rapid Separation ◽  
Maldi Tof Ms ◽  
Maldi Tof Mass Spectrometry ◽  
Maldi Tof ◽  
Spoilage Bacteria ◽  
Beer Spoilage ◽  
Tof Ms

Microfluidics and MALDI-TOF MS is a rapid, high-throughput, and accurate method for the identification of beer spoilage bacteria.

scholarly journals Klebsiella MALDI TypeR: a web-based tool for Klebsiella identification based on MALDI-TOF mass spectrometry

2021 ◽  
pp. 103835
Author(s):  
Sébastien Bridel ◽  
Stephen C. Watts ◽  
Louise M. Judd ◽  
Taylor Harshegyi ◽  
Virginie Passet ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Web Based ◽  
Maldi Tof Mass Spectrometry ◽  
Maldi Tof

Partial Fluorination of Hydroxyl End-Capped Oligoesters Revealed by MALDI−TOF Mass Spectrometry

2001 ◽  
Vol 34 (7) ◽  
pp. 2389-2393 ◽  
Author(s):  
Weihua Ming ◽  
Xianwen Lou ◽  
Robert D. van de Grampel ◽  
Joost L. J. van Dongen ◽  
Rob van der Linde
Keyword(s):  
Mass Spectrometry ◽  
Maldi Tof Mass Spectrometry ◽  
Maldi Tof
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