Chemical fingerprint analysis for quality control of Fructus Aurantii Immaturus based on HPLC-DAD combined with chemometric methods

2010 ◽  
Vol 2 (12) ◽  
pp. 2002 ◽  
Author(s):  
Xiaona Xu ◽  
Junhui Jiang ◽  
Yizeng Liang ◽  
Lunzhao Yi ◽  
Jinle Cheng
Keyword(s):  
Quality Control ◽  
Chemometric Methods ◽  
Fingerprint Analysis ◽  
Chemical Fingerprint

Chemical fingerprint analysis for quality control and identification of Ziyang green tea by HPLC

2015 ◽  
Vol 171 ◽  
pp. 405-411 ◽  
Author(s):  
Xiaoye He ◽  
Jianke Li ◽  
Wei Zhao ◽  
Run Liu ◽  
Lin Zhang ◽  
...  
Keyword(s):  
Quality Control ◽  
Green Tea ◽  
Fingerprint Analysis ◽  
Chemical Fingerprint

Quantitative and chemical fingerprint analysis for quality control of Rhizoma Coptidischinensis based on UPLC-PAD combined with chemometrics methods

Phytomedicine ◽  
2009 ◽  
Vol 16 (10) ◽  
pp. 950-959 ◽  
Author(s):  
Wei-Jun Kong ◽  
Yan-Ling Zhao ◽  
Xiao-He Xiao ◽  
Cheng Jin ◽  
Zu-Lun Li
Keyword(s):  
Quality Control ◽  
Fingerprint Analysis ◽  
Chemical Fingerprint

Chemical Fingerprint Analysis for Quality Control ofHerba EphedraeBased on HPLC-DAD Combined with Chemometrics Methods

2012 ◽  
Vol 45 (13) ◽  
pp. 1824-1835 ◽  
Author(s):  
Xiufen Ren ◽  
Yizeng Liang ◽  
Xiaoning Li ◽  
Haiyang Yi ◽  
Zhimin Zhang
Keyword(s):  
Quality Control ◽  
Fingerprint Analysis ◽  
Chemical Fingerprint

Chemical Fingerprint Analysis and Simultaneous Determination of Nucleosides and Amino Acids in Kang Fu Xin Liquid by High Performance Liquid Chromatography with Diode Array Detector

2020 ◽  
Vol 16 (7) ◽  
pp. 831-843
Author(s):  
Yuwen Wang ◽  
Shuping Li ◽  
Liuhong Zhang ◽  
Shenglan Qi ◽  
Huida Guan ◽  
...  
Keyword(s):  
Amino Acids ◽  
Quality Control ◽  
Uric Acid ◽  
Control Method ◽  
Principal Component ◽  
Gradient Elution ◽  
Hplc Method ◽  
Array Detector ◽  
Fingerprint Analysis ◽  
Wavelength Switching

Background and Objective: Kang Fu Xin liquid (KFX) is an official preparation made from the ethanol extract product from P. Americana. The present quality control method cannot control the quality of the preparation well. The aim of the present study is to establish a convenient HPLC method for multicomponents determination combined with fingerprint analysis for quality control of KFX. Methods: An HPLC-DAD method with gradient elution and detective wavelength switching program was developed to establish HPLC fingerprints of KFX, and 38 batches of KFX were compared and evaluated by similarity analysis (SA), hierarchical clustering analysis (HCA), and principal component analysis (PCA). Meanwhile, six nucleosides and three amino acids, including uracil, hypoxanthine, uric acid, adenosine, xanthine, inosine, tyrosine, phenylalanine and tryptophan in KFX were determined based on the HPLC fingerprints. Results: An HPLC method assisted with gradient elution and wavelength switching program was established and validated for multicomponents determination combined with fingerprint analysis of KFX. The results demonstrated that the similarity values of the KFX samples were more than 0.845. PCA indicated that peaks 4 (hypoxanthine), 7 (xanthine), 9 (tyrosine), 11, 13 and 17 might be the characteristic contributed components. The nine constituents in KFX, uracil, hypoxanthine, uric acid, adenosine, xanthine, inosine, tyrosine, phenylalanine and tryptophan, showed good regression (R2 > 0.9997) within test ranges and the recoveries of the method for all analytes were in the range from 96.74 to 104.24%. The limits of detections and quantifications for nine constituents in DAD were less than 0.22 and 0.43 μg•mL-1, respectively. Conclusion: The qualitative analysis of chemical fingerprints and the quantitative analysis of multiple indicators provide a powerful and rational way to control the KFX quality for pharmaceutical companies.

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