Modeling the retention behavior of analytes in RPLC with mixed solvent mobile phases using Jouyban-Acree and Abraham models

2010 ◽  
Vol 2 (9) ◽  
pp. 1286 ◽  
Author(s):  
Abolghasem Jouyban ◽  
Somaieh Soltani ◽  
Anahita Fathi-Azarbaijani ◽  
William E. Acree Jr.
Keyword(s):  
Mixed Solvent ◽  
Retention Behavior ◽  
Mobile Phases

Application of Mobile Phases Containing Ionic Liquid for HPLC Analysis of Selected Isoquinoline Alkaloids

2017 ◽  
Vol 100 (6) ◽  
pp. 1652-1659 ◽  
Author(s):  
Anna Petruczynik ◽  
Justyna Misiurek ◽  
Tomasz Tuzimski ◽  
Monika Waksmundzka-Hajnos
Keyword(s):  
Ionic Liquid ◽  
Stationary Phases ◽  
Reversed Phase ◽  
System Efficiency ◽  
Organic Modifier ◽  
Optimal Conditions ◽  
Retention Behavior ◽  
Isoquinoline Alkaloids ◽  
Mobile Phases ◽  
Chemical Groups

Abstract An HPLC procedure on a polar reversed-phase column with mobile phases containing ionic liquid (IL) was developed for the analysis of selected alkaloids from different chemical groups. We aimed to obtain optimal conditions for the separation of alkaloids because widely used silica-based stationary phases exhibit a silanol effect, rendering analysis of basic analytes extremely difficult. Retention, separation selectivity, peak symmetry, and system efficiency were examined in various eluent systems containing different concentrations of IL and acetonitrile. The obtained results revealed substantial influence from the concentrations of IL, the organic modifier, and temperature on the retention behavior of the investigated alkaloids. The most selective and efficient chromatographic systems were applied for the analysis of several alkaloids in a plant extract.

scholarly journals Normal and reversed phase thin-layer chromatography of new 16, 17-secoestrone derivatives

2003 ◽  
Vol 68 (1) ◽  
pp. 57-64 ◽  
Author(s):  
Marijana Acanski ◽  
Suzana Jovanovic-Santa ◽  
Lidija Jevric
Keyword(s):  
Thin Layer ◽  
Stationary Phase ◽  
Thin Layer Chromatography ◽  
Silica Gel ◽  
Reversed Phase ◽  
Retention Behavior ◽  
Mobile Phases ◽  
Layer Chromatography

The retention behavior and separation ability of a series of new 16,17-secoestrone derivatives has been studied on silica gel, alumina and C-18 silica gel layers with non-aqueous and aqueous-organic mobile phases. The retention behavior and separation ability are discussed in terms of the nature of the solute, eluent and stationary phase.

scholarly journals Relationships Between LC Retention, Octanol–Water Partition Coefficient, and Fungistatic Properties of 2-(2,4-Dihydroxyphenyl)benzothiazoles

2004 ◽  
Vol 87 (3) ◽  
pp. 579-586 ◽  
Author(s):  
Joanna Matysiak ◽  
Andrzej Niewiadomy ◽  
Bogdan Senczyna ◽  
Anna Żabińska ◽  
Jan K Rózyło
Keyword(s):  
Mobile Phase ◽  
High Performance ◽  
Hydrophobic Surface ◽  
Quantitative Structure Activity Relationship ◽  
Log P ◽  
Retention Behavior ◽  
Hydrophobicity Index ◽  
Mobile Phases ◽  
Chromatographic Parameters ◽  
Mic Minimum Inhibitory Concentration

Abstract The retention behavior of newly synthesized compounds with antimycotic activity from the 2-(2,4-dihydroxyphenyl)benzothiazole group by high-performance liquid chromatography has been investigated. RP-18 stationary phase and methanol–acetate buffer aqueous mobile phases at pH 4 and 7.4 have been used. In the case of the mobile phase at pH 7.4, higher concentrations of water can be applied than at pH 4. The studied compounds showed regular retention behavior, their log k values decreasing linearly with an increasing concentration of methanol in the mobile phase. On the basis of these relationships, the lipophilicity (log kw), specific hydrophobic surface area (S), and isocratic chromatographic hydrophobicity index (φ0) were determined. Similar log kw values and sensitivity to changes in the structure of compounds studied for both mobile phases have been found. Moderate correlations between the chromatographic parameters and the calculated octanol-water log P values were found. Finally, the lipophilicity parameters were compared with the fungistatic properties of compounds expressed by log MIC (minimum inhibitory concentration) values to find quantitative structure activity relationship equations.

scholarly journals Retention behaviour of some estradiol derivatives on alumina in normal phase chromatrography

2003 ◽  
Vol 68 (3) ◽  
pp. 163-170 ◽  
Author(s):  
Marijana Acanski
Keyword(s):  
Mobile Phase ◽  
Volume Fraction ◽  
Retention Behavior ◽  
Relative Retention ◽  
Linear Relationships ◽  
Mobile Phases ◽  
Binary Mobile Phases ◽  
Estradiol Derivatives ◽  
Rf Values ◽  
The Relationship

The retention constants of variously substituted estradiol derivatives were measured as a function of the composition of various binary mobile phases in order to study the relationship between chemical structure and retention behavior in TLC on alumina. The slopes and intercepts of the linear relationships between the retention constant RM and the logarithm of the volume fraction of the polar mobile phase component were calculated and are discussed in relation to the characteristics of the solute and mobile phase. The RF values and relative retention ?RM of the compounds depend largely on the retention behavior of their substituents. The results obtained in this investigation are compared with the results of the same derivatives obtained in previous investigations on silica gel.

scholarly journals Estimation of lipophilicity and design of new 17β-carboxamide glucocorticoids using RP-HPLC and quantitative structure-retention relationships analysis

2021 ◽  
Author(s):  
Vladimir Dobričić ◽  
Jelena Bošković ◽  
Dragana Vukadinović ◽  
Sote Vladimirov ◽  
Olivera Čudina
Keyword(s):  
Inflammatory Activity ◽  
Chromatographic Behavior ◽  
Quantitative Structure ◽  
Retention Behavior ◽  
Rp Hplc ◽  
Mobile Phases ◽  
Structure Retention ◽  
Lipophilicity Prediction ◽  
Cyano Column ◽  
Quantitative Structure Retention Relationships

AbstractEight 17β-carboxamide glucocorticoids with local anti-inflammatory activity were selected and their retention behavior tested in six RP-HPLC systems (I–VI). logkw, a, and φ0 parameters were calculated and correlation with previously determined logPo/w values was examined. RP-HPLC system IV, which consisted of cyano column and methanol–water mobile phases (50:50, 60:40, 70:30, and 80:20, v/v), was selected as the most reliable for lipophilicity prediction and used for the analysis of chromatographic behavior of remaining fourteen 17β-carboxamide glucocorticoids. Quantitative structure-retention relationships analysis was performed and PLS(logkw) model was selected as the most statistically significant. On the basis of selected model and interpretation of corresponding descriptors, new derivatives with higher logkw values and higher expected lipophilicity were designed.

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