Optimization and validation of spectrofluorimetric method for the determination of clomipramine hydrochloride via ion-pair complexation with alizarin red S

2010 ◽  
Vol 2 (5) ◽  
pp. 513 ◽  
Author(s):  
Nafisur Rahman ◽  
Nasheed Afaq
Keyword(s):  
Ion Pair ◽  
Alizarin Red S ◽  
Alizarin Red ◽  
Spectrofluorimetric Method

scholarly journals DETERMINATION OF FLUOXETINE HYDROCHLORIDE VIA ION PAIR COMPLEXATION WITH ALIZARIN RED S

2018 ◽  
Vol 75 (6) ◽  
pp. 1293-1303
Author(s):  
Constantinescu Clementina ◽  
Neagu Alexandra ◽  
Uivarosi Valentina
Keyword(s):  
Ion Pair ◽  
Alizarin Red S ◽  
Alizarin Red ◽  
Fluoxetine Hydrochloride

scholarly journals Use of Alizarin Red S as an Ion-Pair Reagent for the Spectrophotometric Assay of Fexofenadine in Pharmaceuticals and in Spiked Human Urine

2012 ◽  
Vol 2012 ◽  
pp. 1-10
Author(s):  
Madihalli S. Raghu ◽  
Kanakapura Basavaiah ◽  
Kudige N. Prashanth ◽  
Kankapura B. Vinay
Keyword(s):  
Human Urine ◽  
Standard Addition ◽  
Cost Effective ◽  
Molar Absorptivity ◽  
Ion Pair ◽  
Alizarin Red S ◽  
Alizarin Red ◽  
Spectrophotometric Methods ◽  
Spiked Human Urine

The present study describes two simple, rapid, selective, and cost-effective spectrophotometric methods for the determination of an antiallergic drug, fexofenadine hydrochloride (FFH), in bulk drug, tablets, and in spiked human urine. The first method (method A) is based on the formation of yellow-colored ion-pair complex between FFH and alizarin red S (AZS) in acid medium which was extracted into dichloromethane, and the absorbance was measured at 440 nm. The second method (method B) is based on the breaking of the yellow FFH–AZS ion-pair complex in alkaline medium followed by the measurement of the violet-colored free dye at 590 nm. Under the optimized conditions, Beer’s law is obeyed over the concentration ranges of 0.4–12.0 and 0.2–3.5 μg  FFH for method A and method B, respectively, and the corresponding molar absorptivity values are 3.80 × 104 and 1.61 × 105 L . The Sandell’s sensitivity, detection, and quantification limits are also reported. The proposed methods were successfully applied to the determination of FFH in pure drug and commercial tablets. The accuracy and reliability of the proposed methods were further established by recovery studies via standard addition technique.

Spectrophotometric determination of aluminium with alizarin red S sensitized with polyvinylpyrrolidone

1983 ◽  
Vol 149 ◽  
pp. 379-383 ◽  
Author(s):  
J. Hernández-Méndez ◽  
R. Carabias-Martínez ◽  
B. Moreno-Cordero ◽  
L. Gutiérrez-Dávila
Keyword(s):  
Spectrophotometric Determination ◽  
Alizarin Red S ◽  
Alizarin Red

Electrocatalytic determination of chlorpromazine drug using Alizarin Red S as a mediator on the glassy carbon electrode

2011 ◽  
Vol 47 (1) ◽  
pp. 34-41 ◽  
Author(s):  
Mohammad Ali Karimi ◽  
Abdolhamid Hatefi-Mehrjardi ◽  
Mohammad Mazloum Ardakani ◽  
Reza Behjatmanesh Ardakani ◽  
Mohammad Hossein Mashhadizadeh ◽  
...  
Keyword(s):  
Glassy Carbon Electrode ◽  
Glassy Carbon ◽  
Alizarin Red S ◽  
Carbon Electrode ◽  
Alizarin Red ◽  
Electrocatalytic Determination

scholarly journals Spectrophotometric Determination of Toxic Elements (Cadmium) in Aqueous Media

2011 ◽  
pp. 1-12 ◽  
Author(s):  
MR Ullah ◽  
M Enamul Haque
Keyword(s):  
Sodium Salt ◽  
Biological Samples ◽  
Chemical Engineering ◽  
Sulphonic Acid ◽  
Alizarin Red S ◽  
Standard Reference Materials ◽  
Complexing Agents ◽  
Alizarin Red ◽  
Acid Sodium Salt

Trace amount of toxic element (Cadmium) was determined by spectrophotometer method using 1, 2-dihydroxy anthraquinone-3-sulphonic acid, sodium salt. (Alizarin red S) as a new spectrophotometer reagent. Alizarin red S reacts in slightly acidic solution (0.005 - 0.05M H2SO4) with cadmium to give a deep greenish yellow chelate which has an absorption maximum at 422 nm. The reaction is instantaneous and absorbance remains stable for over 24hrs. The average molar absorption co-efficient and sandell's sensitivity were found to be 2.24 103L mol-1 cm-1 and 20 ng cm 2 of Cd respectively. Linear calibration graphs were obtained for 0.1 - 40μg mL-1 of Cd. The stoichiometric composition of the chelate is 1 : 2 (Cd : Alizarin red S). Large excess of over 50 cations, anions, and some common complexing agents (e.g EDTA, oxalate, citrate phosphate, thio-urea, SCN-) do not interfere in the determination. The method was successfully used in the determination of cadmium in Several Standard Reference Materials (alloys, steels and water) as well as in some environmental waters (In land and surface), biological samples (human blood and urine), soil samples and complex synthetic mixtures. The method has high precision and accuracy. (S = ±0.01 for 0.5 μg mL-1. Keywords spectrophotometry; cadmium determination; 1, 2-dihydroxy anthraquinone-3-sulphonic acid; sodium salt (Alizarin red S); alloy; steel; environmental; biological; samples; soil samplesDOI: 10.3329/jce.v25i0.7233Journal of Chemical Engineering, IEB Vol. ChE. 25, No. 1, December 2010 pp.1-12

Spectrofluorimetric determination of boron in soils, plants and natural waters with Alizarin Red S

The Analyst ◽  
1992 ◽  
Vol 117 (7) ◽  
pp. 1189-1191 ◽  
Author(s):  
Ana M. García Campaña ◽  
Fermín Alés Barrero ◽  
Manuel Román Ceba
Keyword(s):  
Natural Waters ◽  
Alizarin Red S ◽  
Alizarin Red ◽  
Spectrofluorimetric Determination

scholarly journals Development and validation of a new spectrofluorimetric method for the determination of some beta-blockers through fluorescence quenching of eosin Y. Application to content uniformity test

2016 ◽  
Vol 14 (1) ◽  
pp. 258-266 ◽  
Author(s):  
Sayed M Derayea ◽  
Mahmoud A Omar ◽  
Mohamed Aboel-Kasem Abdel-Lateef ◽  
Ahmed I. Hassan
Keyword(s):  
Fluorescence Quenching ◽  
Beta Blockers ◽  
Dosage Forms ◽  
Ion Pair ◽  
Content Uniformity ◽  
Eosin Y ◽  
Pharmaceutical Dosage Forms ◽  
Quenching Effect ◽  
Spectrofluorimetric Method

AbstractA simple, rapid, sensitive and economic spectrofluorimetric method has been developed and validated for determination of some β-adrenergic blocking agents namely; betaxolol hydrochloride (BTX), carvedilol (CAR), labetalol hydrochloride (LBT), nebivolol hydrochloride (NEB) and propranolol hydrochloride (PRO). The method is based on the quenching effect of the cited drugs on the fluorescence intensity of eosin Y at pH 3.4 (acetate buffer). The fluorescence quenching is due to the formation of an ion-pair complex and was measured without extraction at 545 nm (λex. 301.5 nm). The factors affecting the formation of the ion-pair complex were carefully studied and optimized. Under the optimal conditions, the linear ranges for the relationship between the fluorescence quenching value and the concentration of the investigated drugs were 100-2500, 150-2500 and 50-2250 ng mL-1 for (BTX, CAR), (LBT, NEB) and (PRO) respectively. The method was validated according to ICH guidelines and was applied for determination of the cited drugs in pharmaceutical dosage forms with excellent recoveries. In addition, content uniformity testing of some commercial dosage forms was checked by the proposed method.

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