Electrochemical detection of extracellular hydrogen peroxide released from RAW 264.7 murine macrophage cells based on horseradish peroxidase–hydroxyapatite nanohybrids

The Analyst ◽  
2011 ◽  
Vol 136 (6) ◽  
pp. 1116-1123 ◽  
Author(s):  
Chunyun Li ◽  
Hui Zhang ◽  
Ping Wu ◽  
Zhunan Gong ◽  
Guanglin Xu ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Horseradish Peroxidase ◽  
Electrochemical Detection ◽  
Raw 264.7 ◽  
Murine Macrophage ◽  
Macrophage Cells

scholarly journals Effects of bitter melon on inflammatory molecules induced by lipopolysaccharides (LPS) on RAW 264.7 murine macrophage cells

2008 ◽  
Vol 22 (S1) ◽  
Author(s):  
Amy Seewald ◽  
Fatima Pina ◽  
Kandice Beverly ◽  
Brenda Jane Smith ◽  
Edralin Lucas
Keyword(s):  
Raw 264.7 ◽  
Bitter Melon ◽  
Murine Macrophage ◽  
Macrophage Cells ◽  
Inflammatory Molecules

scholarly journals Anti-Oxidative Effect of Weak Alkaline Reduced Water in RAW 264.7 Murine Macrophage Cells

Processes ◽  
2021 ◽  
Vol 9 (11) ◽  
pp. 2062
Author(s):  
Thuy Thi Trinh ◽  
Ailyn Fadriquela ◽  
Johny Bajgai ◽  
Subham Sharma ◽  
Md. Habibur Rahman ◽  
...  
Keyword(s):  
Tap Water ◽  
Daily Intake ◽  
Mapk Signaling ◽  
Raw 264.7 ◽  
Dependent Manner ◽  
Murine Macrophage ◽  
Concentration Dependent Manner ◽  
High Hydrogen ◽  
Macrophage Cells ◽  
Reduced Water

Excessive oxidative stress (OS) is a common cause of various diseases such as cancer, diabetes, and obesity; thus, an anti-oxidative solution is essential for the improvement of human health. Increasing evidence suggests that alkaline reduced water (ARW), especially between pH 9.5–10.0, has antioxidant capacity; however, relatively few studies have reported the effect of weak ARW at pH 8.5 on OS, especially in vitro. This study was conducted to evaluate the anti-oxidative efficacy of weak ARW with negative oxidation-reduction potential (ORP) and relatively high hydrogen (H2) concentration, as compared to tap water (TW) and ARW at pH 9.5. RAW 264.7 murine macrophage cells, stimulated by hydrogen peroxide (H2O2) and lipopolysaccharide (LPS) to induce OS, were used as a control (Con) and then treated with TW and ARW at pH 8.5 (ARW_8.5) and pH 9.5 (ARW_9.5) at different concentrations (0.1%, 1%, and 10% v/v). Results showed that cell viability was significantly restored after treatment with both ARW_8.5 and ARW_9.5 compared to Con/H2O2 and Con/LPS, while TW treatment did not induce significant changes. Levels of reactive oxygen species (ROS), nitric oxide (NO), Ca2+, catalase, and glutathione peroxide (GPx) showed significant differences in a concentration-dependent manner in ARW_8.5 and ARW_9.5 groups compared to Con/H2O2 and Con/LPS groups. Likewise, the expression of p-p38, p-JNK, and p-ERK was also significantly reduced in the ARW-treated groups, but not in the TW group. In conclusion, ARW_8.5 exhibited anti-oxidative effects through the regulation of the MAPK signaling pathway in RAW 264.7 murine macrophage cells, indicating the health-promoting potential of weak ARW through daily intake.

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