Remarkable effect of mobile phase buffer on the SEC-ICP-AES derived Cu, Fe and Zn-metalloproteome pattern of rabbit blood plasma

Metallomics ◽  
2010 ◽  
Vol 2 (7) ◽  
pp. 460 ◽  
Author(s):  
Elham Zeini Jahromi ◽  
Wade White ◽  
Qiao Wu ◽  
Raghav Yamdagni ◽  
Jürgen Gailer
Keyword(s):  
Blood Plasma ◽  
Mobile Phase ◽  
Remarkable Effect ◽  
Rabbit Blood ◽  
Rabbit Blood Plasma

Preparation of purified erythropoietin from rabbit blood plasma

1981 ◽  
Vol 92 (6) ◽  
pp. 1660-1661
Author(s):  
S. N. Osipova ◽  
V. V. Mezhevikin ◽  
I. I. Gitel'zon
Keyword(s):  
Blood Plasma ◽  
Rabbit Blood ◽  
Rabbit Blood Plasma

The carbamyl phosphate content of rabbit blood plasma

1964 ◽  
Vol 107 (3) ◽  
pp. 544-549 ◽  
Author(s):  
Annemarie Herzfeld ◽  
Sally E. Hager ◽  
Mary Ellen Jones
Keyword(s):  
Blood Plasma ◽  
Phosphate Content ◽  
Carbamyl Phosphate ◽  
Rabbit Blood ◽  
Rabbit Blood Plasma

Quantitative Liquid Chromatographic Method Using Fluorescence Detection for Determining Zearalenone and Its Metabolites in Blood Plasma and Urine

1985 ◽  
Vol 68 (4) ◽  
pp. 632-635 ◽  
Author(s):  
Monica E Olsen ◽  
Hans I Pettersson ◽  
Kerstin A Sandholm ◽  
Karl-Heinz C Kiessling
Keyword(s):  
Blood Plasma ◽  
Mobile Phase ◽  
Chloroform Extract ◽  
Excitation Wavelength ◽  
Fluorescence Detector ◽  
Standard Ml ◽  
Blood And Urine Samples ◽  
Almost All ◽  
Water Saturated ◽  
Liquid Chromatographic

Abstract The liquid chromatographic (LC) method described, suitable for use with both blood plasma and urine, is applicable for determination of zearalenone and α-zearalenol at levels as low as 0.5 ng/mL plasma and 5 ng/mL urine. The sample is incubated overnight β with glucuronidase to analyze for both conjugated and unconjugated forms of zearalenone. The next day, the sample is acidified with H3P04, extracted with chloroform, and evaporated to dryness. The residue is dissolved in toluene and loaded onto a silica gel cartridge which is washed with toluene and eluted with toluene-acetone (88 + 12). The eluate is evaporated, and the residue is dissolved in chloroform, extracted with 0.18M NaOH, neutralized with H3PO4, and re-extracted with chloroform. The chloroform extract is evaporated, dissolved in mobile phase for LC, and injected onto a normal phase column under the following chromatographic conditions: mobile phase of water-saturated dichloromethane containing 2% 1-propanol, and fluorescence detector, excitation wavelength 236 nm, and 418 nm cut-off emission filter. Recoveries of zearalenone and its metabolites from blood plasma and urine are 80-89% in the range 2.0-10 ng standard/mL plasma, and 81-90% in the range 10-30 ng standard/mL urine. This method was used to analyze blood and urine samples from a pig fed zearalenone-contaminated feed (5 mg/kg), corresponding to 80 μg/kg body weight. Zearalenone was rapidly metabolized to a-zearalenol, which appeared in the blood only 30 min after feeding. Almost all zearalenone and αzearalenol was found conjugated with glucuronic acid in both blood plasma and urine.

Comments on spectrofluorometric assay of angiotensin-converting enzyme activity in blood plasma of different species

1986 ◽  
Vol 51 (10) ◽  
pp. 2280-2284
Author(s):  
Assia C. Shisheva ◽  
Ognian C. Ikonomov ◽  
Luben M. Sirakov
Keyword(s):  
Blood Plasma ◽  
Hydrolytic Activity ◽  
Converting Enzyme ◽  
Rat Blood ◽  
Spectrofluorometric Determination ◽  
Ace Activity ◽  
Angiotension Converting Enzyme ◽  
Hydrolysis Of ◽  
Rabbit Blood Plasma

The activity of the angiotension -converting enzyme (ACE) in human, dog, rabbit, and rat blood plasma was assayed by spectrofluorometric determination of the product liberated by enzymatic cleavage (L-His-L-Leu). In parallel experiments the hydrolysis of L-His-L-Leu by blood plasma was examined. The hydrolytic activity of rat blood plasma was high and therefore lower values of ACE activity were obtained; the use of the spectrofluorometric assay with rat blood plasma is therefore problematic. By contrast, L-His-L-Leu was not degraded by human, dog, and rabbit blood plasma and the spectrofluorometric determination of this peptide can thus be used to advantage to assay the ACE activity of blood plasma samples of these species.

scholarly journals The study of intestinal deсametoxin absorption in rats and its influence on blood cholinesterase activity in rabbits

2015 ◽  
Vol 13 (1) ◽  
pp. 45-51
Author(s):  
Nataliya Nikolaevna Derkach ◽  
Sergey Yur'yevich Shtrygol' ◽  
Ol'ga Olegovna Koyro ◽  
Nikolay Evstakhievich Blazheevskiy
Keyword(s):  
Cholinesterase Activity ◽  
Cholinesterase Inhibition ◽  
Albino Rats ◽  
Enzymatic Method ◽  
Rat Intestine ◽  
Gastrointestinal Infections ◽  
Rabbit Blood ◽  
Single Oral Administration ◽  
Rabbit Blood Plasma

The possibility of (0.02 % solution) absorbtion was evaluated under the conditions of small intestine perfusion in situ in the experiments with albino rats. Decametoxin content was determined in the perfusate by kinetic enzymatic method using the reaction of exogenous cholinesterase inhibition. Decametoxin influence on the activity of endogenous cholinesterase of the rabbit blood plasma was also studied after the single oral administration of the drug at a dose of 6 ml/kg that corresponds to the dose of 1.2 mg/kg. It has been shown that decametoxin is almost not absorbed in rat intestine and does not influence on cholinesterase activity of the rabbit blood that substantiate the possibility of its usage in gastrointestinal infections.

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