The evaluation of the amount of cis- and trans-urocanic acid in the stratum corneum by Raman spectroscopy

2010 ◽  
Vol 9 (5) ◽  
pp. 730 ◽  
Author(s):  
Mariko Egawa ◽  
Junko Nomura ◽  
Haruhi Iwaki
Keyword(s):  
Raman Spectroscopy ◽  
Stratum Corneum ◽  
Urocanic Acid ◽  
Cis And Trans

scholarly journals Factors controlling the expressed activity of histidine ammonia-lyase in the epidermis and the resulting accumulation of urocanic acid

1981 ◽  
Vol 194 (3) ◽  
pp. 829-838 ◽  
Author(s):  
I R Scott
Keyword(s):  
Stratum Corneum ◽  
Living Cells ◽  
Urocanic Acid ◽  
Residual Protein ◽  
Thickness Skin ◽  
Lyase Activity ◽  
Part Thickness ◽  
Natural Acidity ◽  
Marked Dependence ◽  
Skin Samples

The synthesis of urocanic acid by histidine ammonia-lyase in guinea-pig epidermis was investigated in various ways. 1. In epidermal homogenates the enzyme obeys Michaelis-Menten kinetics and shows marked dependence of its activity of pH, such that below pH 6 it is inactive. 2. Part-thickness skin samples cultured with radioactive histidine do not accumulate detectable radioactive urocanic acid during 3 days in culture. 3. Very little histidine ammonia-lyase activity can be detected in the living cells of the epidermis. The enzyme is almost completely restricted to the dead cells of the stratum corneum. 4. Radioactive histidine injected into living animals does not result immediately in the accumulation of radioactive urocanic acid. By 3 days after the injection, however, both radioactive urocanic acid and histidine appear, apparently at the expense of radioactive proteins, 5. In isolated stratum corneum, the residual histidine can be converted into urocanic acid by the histidine ammonia-lyase in the tissue only if the natural acidity of the tissue is neutralized. It is concluded from these observations that the biosynthesis of urocanic acid occurs naturally only in the stratum corneum, which contains only dead cells. The amount of urocanic acid accumulated is limited by the availability of free histidine produced by proteolysis of residual protein in these cells and by the penetration into the stratum corneum of the ‘acid mantle’ of the skin.

In vivo intra- and inter-individual variability study of human stratum corneum by confocal Raman spectroscopy

2016 ◽  
Vol 87 ◽  
pp. 199-206
Author(s):  
Laurita dos Santos ◽  
Joao Lucas Rangel ◽  
Vamshi Krishna Tippavajhala ◽  
Michely Glenda Pereira da Silva ◽  
Borys Mogilevych ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Stratum Corneum ◽  
Individual Variability ◽  
Confocal Raman Spectroscopy ◽  
Human Stratum Corneum ◽  
Confocal Raman ◽  
Variability Study

Raman spectroscopy: feasibility of in vivo survey of stratum corneum lipids, effect of natural aging

2012 ◽  
Vol 22 (1) ◽  
pp. 36-41 ◽  
Author(s):  
Ali Tfayli ◽  
Emmanuelle Guillard ◽  
Michel Manfait ◽  
Arlette Baillet-Guffroy
Keyword(s):  
Raman Spectroscopy ◽  
Stratum Corneum ◽  
Natural Aging ◽  
Stratum Corneum Lipids

Effect of mixed surfactants on stratum corneum: a drying stress and Raman spectroscopy study

2014 ◽  
Vol 36 (4) ◽  
pp. 379-385 ◽  
Author(s):  
P. Purohit ◽  
P. Chandar ◽  
A. Vilinska ◽  
K. P. Ananthapadmanabhan ◽  
P. Somasundaran
Keyword(s):  
Raman Spectroscopy ◽  
Stratum Corneum ◽  
Mixed Surfactants ◽  
Spectroscopy Study
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