Peptide quantitation using primary amine selective metal chelation labels for mass spectrometry

2010 ◽  
Vol 46 (30) ◽  
pp. 5479 ◽  
Author(s):  
Thomas J. Kerr ◽  
John A. McLean
Keyword(s):  
Mass Spectrometry ◽  
Primary Amine ◽  
Metal Chelation ◽  
Peptide Quantitation

An Improved, Versatile, and Easily Scalable Synthesis of Sphingomyelins: Application to Stable Isotope Labeling

Synthesis ◽  
2020 ◽  
Vol 52 (14) ◽  
pp. 2106-2110
Author(s):  
Nicolas Philippe ◽  
Serge Pérard ◽  
Franck Le Strat ◽  
Jörg Blankenstein ◽  
Sébastien Roy
Keyword(s):  
Mass Spectrometry ◽  
Stable Isotope ◽  
Primary Amine ◽  
Isotope Labeling ◽  
Stable Isotope Labeling ◽  
Scalable Synthesis

With a view to make conveniently labeled mass spectrometry standards available, a set of deuterated sphingomyelins were prepared by a new expedient, flexible, robust, scalable, and high-yielding synthetic scheme starting from 2-azido-3-O-benzoylsphingosine as the key intermediate. Unlike previously published procedures, this work emphasizes the benefit arising from the choice of the azido function as a masking group for the reactive primary amine during the troublesome, though crucial, phosphorylation step.

Study of formation and fragmentation of ionic complexes of polydentate ligands with Al(III) and glycerol by fast atom bombardment mass spectrometry. Part 1: Polydentate ligands

1996 ◽  
Vol 74 (11) ◽  
pp. 2221-2228
Author(s):  
Mandapati Saraswathi ◽  
Jack M. Miller
Keyword(s):  
Mass Spectrometry ◽  
Fast Atom Bombardment ◽  
Polyethylene Glycols ◽  
Metal Chelation ◽  
Ionic Complexes ◽  
Polydentate Ligands ◽  
Fragment Ions ◽  
Fab Mass Spectrometry ◽  
Aluminum Ions ◽  
Complexation Reactions

The complexation reactions of aluminum ions with polydentate ligands such as 12-crown-4,15-crown-5,18-crown-6, 1,10-dithia-18-crown-6, dicyclohexyl-18-crown-6, dibenzo-18-crown-6, and dibenzo-24-crown-8 and acyclic analogs mono-, di-, tri-, tetra-, penta-, and hexaethylene glycols were studied using FAB mass spectrometry. These cyclic ligands form (M + 117)+, (M + 157)+, (M + 231)+, and (M + 253)+ ions with different aluminum-containing species. Collisionally activated dissociations of these adduct ions gave fragment ions, initially due to the loss of ligands directly attached to aluminum, followed by insertion of aluminum into the remaining ligand skeleton. Further fragmentation of the metal-containing species gave ions corresponding to consecutive losses of C2H4O units. Fragmentations of deuterium-labelled ions were used to help in establishing fragmentation pathways. Selectivity towards metal chelation is observed in this order: 12-crown-4 < 15-crown-5 < 18-crown-6. The elemental compositions of adduct ions were confirmed by high-resolution measurements. The formation of (M + Al − 2H)+ ion, obtained by the displacement of two hydroxy protons, is more favored for tetra- and pentaethylene glycols. Key words: crown ethers, polyethylene glycols, aluminum(III)–glycerol, ionic complexes and ion dissociations.

Peptide quantitation with methyl iodide isotopic tags and mass spectrometry

The Analyst ◽  
2010 ◽  
Vol 135 (6) ◽  
pp. 1456 ◽  
Author(s):  
Voislav Blagojevic ◽  
Nickholas Zhidkov ◽  
Samuel Tharmaratnam ◽  
Van Thong Pham ◽  
Harvey Kaplan ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Methyl Iodide ◽  
Peptide Quantitation

scholarly journals Investigation of Elemental Mass Spectrometry in Pharmacology for Peptide Quantitation at Femtomolar Levels

PLoS ONE ◽  
2016 ◽  
Vol 11 (6) ◽  
pp. e0157943 ◽  
Author(s):  
Emmanuelle Cordeau ◽  
Carine Arnaudguilhem ◽  
Brice Bouyssiere ◽  
Agnès Hagège ◽  
Jean Martinez ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Peptide Quantitation ◽  
Elemental Mass ◽  
Elemental Mass Spectrometry
Sign in / Sign up

Export Citation Format

Share Document