One-step covalent microcontact printing approach to produce patterns of lactate oxidase

E. Casero; M. D. Petit-Domínguez; A. M. Parra-Alfambra; M. J. Gismera; F. Pariente; E. Lorenzo; L. Vázquez

doi:10.1039/b922805e

One-step synthesis of patterned polymer brushes by photocatalytic microcontact printing

Chemical Communications ◽

10.1039/c4cc08646e ◽

2015 ◽

Vol 51 (6) ◽

pp. 1027-1030 ◽

Cited By ~ 14

Author(s):

Friederike Kettling ◽

Benjamin Vonhören ◽

Jennifer A. Krings ◽

Susumu Saito ◽

Bart Jan Ravoo

Keyword(s):

Polymer Brushes ◽

Microcontact Printing ◽

One Step ◽

Novel Method

A novel method to prepare microstructured polymer brushes using TiO2 nanoparticles and photocatalytic microcontact printing is described.

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Layered Double Hydroxide-Modified Organic Electrochemical Transistor for Glucose and Lactate Biosensing

Sensors ◽

10.3390/s20123453 ◽

2020 ◽

Vol 20 (12) ◽

pp. 3453 ◽

Cited By ~ 1

Author(s):

Isacco Gualandi ◽

Marta Tessarolo ◽

Federica Mariani ◽

Danilo Arcangeli ◽

Luca Possanzini ◽

...

Keyword(s):

Layered Double Hydroxide ◽

Limit Of Detection ◽

Drain Current ◽

Analyte Concentration ◽

Lactate Oxidase ◽

Amperometric Response ◽

Electrochemical Transistor ◽

One Step ◽

Double Hydroxide ◽

Biosensor Response

Biosensors based on Organic Electrochemical Transistors (OECTs) are developed for the selective detection of glucose and lactate. The transistor architecture provides signal amplification (gain) with respect to the simple amperometric response. The biosensors are based on a poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate) (PEDOT:PSS) channel and the gate electrode is functionalised with glucose oxidase (GOx) or lactate oxidase (LOx) enzymes, which are immobilised within a Ni/Al Layered Double Hydroxide (LDH) through a one-step electrodeposition procedure. The here-designed OECT architecture allows minimising the required amount of enzyme during electrodeposition. The output signal of the biosensor is the drain current (Id), which decreases as the analyte concentration increases. In the optimised conditions, the biosensor responds to glucose in the range of 0.1–8.0 mM with a limit of detection (LOD) of 0.02 mM. Two regimes of proportionality are observed. For concentrations lower than 1.0 mM, a linear response is obtained with a mean gain of 360, whereas for concentrations higher than 1.0 mM, Id is proportional to the logarithm of glucose concentration, with a gain of 220. For lactate detection, the biosensor response is linear in the whole concentration range (0.05–8.0 mM). A LOD of 0.04 mM is reached, with a net gain equal to 400.

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Sonochemically Synthesized ZnO Nanostructure-Based L-Lactate Enzymatic Sensors on Flexible Substrates

MRS Advances ◽

10.1557/adv.2018.76 ◽

2018 ◽

Vol 3 (5) ◽

pp. 277-282 ◽

Cited By ~ 3

Author(s):

Fahmida Alam ◽

Ahmed Hasnain Jalal ◽

Raju Sinha ◽

Yogeswaran Umasankar ◽

Shekhar Bhansali ◽

...

Keyword(s):

Flexible Substrates ◽

Two Dimensional ◽

Lactate Oxidase ◽

Zno Nanostructure ◽

Non Invasive ◽

Highly Sensitive ◽

Isoelectric Points ◽

One Step ◽

Lactate Sensor ◽

Pm 10

ABSTRACTWe report on highly sensitive and flexible L-lactate enzymatic sensors. The sensing materials of this biosensor, two-dimensional (2D) zinc oxide nanoflakes (ZnO NFs), are synthesized on flexible gold(Au)-coated polyethylene terephthalate (PET) substrate using one step sonochemical approach for non-invasive lactate monitoring in human perspiration. ZnO NFs show high isoelectric points (IEP) and biocompatibility. Taking advantage of these unique properties, we immobilized Lactate oxidase (LOx) on the synthesized ZnO NFs. PET/Au/ZnO NFs sensors demonstrated detection of lactate in the range of 10 pM-10 µM for the electrode area of 0.5×0.5 cm2. The sensitivity of this linker free lactate sensor was found 2.23μA/M /cm2 and shows 4 times better response than conventional Au electrode with linker.

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An Interactive Minicomputer Program for the Indexing and Simulation of Electron Diffraction Patterns

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100092670 ◽

1976 ◽

Vol 34 ◽

pp. 542-543

Author(s):

R.P. Goehner ◽

W.T. Hatfield ◽

Prakash Rao

Keyword(s):

Electron Diffraction ◽

Real Time ◽

Pattern Analysis ◽

Flow Diagram ◽

Hard Copy ◽

Time Analysis ◽

Real Time Analysis ◽

Transmission Electron ◽

One Step ◽

Diffraction Patterns

Computer programs are now available in various laboratories for the indexing and simulation of transmission electron diffraction patterns. Although these programs address themselves to the solution of various aspects of the indexing and simulation process, the ultimate goal is to perform real time diffraction pattern analysis directly off of the imaging screen of the transmission electron microscope. The program to be described in this paper represents one step prior to real time analysis. It involves the combination of two programs, described in an earlier paper(l), into a single program for use on an interactive basis with a minicomputer. In our case, the minicomputer is an INTERDATA 70 equipped with a Tektronix 4010-1 graphical display terminal and hard copy unit.A simplified flow diagram of the combined program, written in Fortran IV, is shown in Figure 1. It consists of two programs INDEX and TEDP which index and simulate electron diffraction patterns respectively. The user has the option of choosing either the indexing or simulating aspects of the combined program.

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Nutrient-regulated gene expression in eukaryotes

Biochemical Society Symposium ◽

10.1042/bss0730085 ◽

2006 ◽

Vol 73 ◽

pp. 85-96 ◽

Cited By ~ 8

Author(s):

Richard J. Reece ◽

Laila Beynon ◽

Stacey Holden ◽

Amanda D. Hughes ◽

Karine Rébora ◽

...

Keyword(s):

Gene Expression ◽

Rna Polymerase ◽

Rna Polymerase Ii ◽

Transcriptional Control ◽

Direct Interaction ◽

Signalling Pathways ◽

A Cell ◽

One Step ◽

Higher Eukaryotes ◽

Regulated Gene Expression

The recognition of changes in environmental conditions, and the ability to adapt to these changes, is essential for the viability of cells. There are numerous well characterized systems by which the presence or absence of an individual metabolite may be recognized by a cell. However, the recognition of a metabolite is just one step in a process that often results in changes in the expression of whole sets of genes required to respond to that metabolite. In higher eukaryotes, the signalling pathway between metabolite recognition and transcriptional control can be complex. Recent evidence from the relatively simple eukaryote yeast suggests that complex signalling pathways may be circumvented through the direct interaction between individual metabolites and regulators of RNA polymerase II-mediated transcription. Biochemical and structural analyses are beginning to unravel these elegant genetic control elements.

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