Single-molecule, single-particle fluorescence imaging of TiO2-based photocatalytic reactions

2010 ◽  
Vol 39 (12) ◽  
pp. 4802 ◽  
Author(s):  
Takashi Tachikawa ◽  
Tetsuro Majima
Keyword(s):  
Fluorescence Imaging ◽  
Single Molecule ◽  
Single Particle ◽  
Photocatalytic Reactions

scholarly journals Enhancing the photostability of poly(phenylene ethynylene) for single particle studies

2017 ◽  
Vol 16 (12) ◽  
pp. 1821-1831 ◽  
Author(s):  
C. F. Calver ◽  
B. A. Lago ◽  
K. S. Schanze ◽  
G. Cosa
Keyword(s):  
Fluorescence Imaging ◽  
Single Molecule ◽  
Single Particle ◽  
Phenylene Ethynylene ◽  
Conjugated Polyelectrolytes ◽  
Imaging Studies ◽  
Single Molecule Fluorescence ◽  
Enhanced Photostability ◽  
The Way

Enhanced photostability of conjugated polyelectrolytes achieved by using anti-fading agents opens the way for advanced single molecule fluorescence imaging studies.

scholarly journals Single Molecule Fluorescence Imaging and Blinking

2006 ◽  
Vol 46 (3) ◽  
pp. 164-168
Author(s):  
Hiroaki YOKOTA ◽  
Tetsuichi WAZAWA ◽  
Yoshiharu ISHII
Keyword(s):  
Fluorescence Imaging ◽  
Single Molecule ◽  
Single Molecule Fluorescence

scholarly journals Low-Light Photodetectors for Fluorescence Microscopy

2021 ◽  
Vol 11 (6) ◽  
pp. 2773
Author(s):  
Hiroaki Yokota ◽  
Atsuhito Fukasawa ◽  
Minako Hirano ◽  
Toru Ide
Keyword(s):  
Fluorescence Microscopy ◽  
Fluorescence Imaging ◽  
Single Molecule ◽  
Science Studies ◽  
Single Photon ◽  
Laser Scanning Microscopy ◽  
Oxide Semiconductor ◽  
Wide Field ◽  
Single Molecule Fluorescence ◽  
Low Light

Over the years, fluorescence microscopy has evolved and has become a necessary element of life science studies. Microscopy has elucidated biological processes in live cells and organisms, and also enabled tracking of biomolecules in real time. Development of highly sensitive photodetectors and light sources, in addition to the evolution of various illumination methods and fluorophores, has helped microscopy acquire single-molecule fluorescence sensitivity, enabling single-molecule fluorescence imaging and detection. Low-light photodetectors used in microscopy are classified into two categories: point photodetectors and wide-field photodetectors. Although point photodetectors, notably photomultiplier tubes (PMTs), have been commonly used in laser scanning microscopy (LSM) with a confocal illumination setup, wide-field photodetectors, such as electron-multiplying charge-coupled devices (EMCCDs) and scientific complementary metal-oxide-semiconductor (sCMOS) cameras have been used in fluorescence imaging. This review focuses on the former low-light point photodetectors and presents their fluorescence microscopy applications and recent progress. These photodetectors include conventional PMTs, single photon avalanche diodes (SPADs), hybrid photodetectors (HPDs), in addition to newly emerging photodetectors, such as silicon photomultipliers (SiPMs) (also known as multi-pixel photon counters (MPPCs)) and superconducting nanowire single photon detectors (SSPDs). In particular, this review shows distinctive features of HPD and application of HPD to wide-field single-molecule fluorescence detection.

Fast Fourier-Domain Localization Algorithm of Single Molecule with Nanometer Resolution for Super-Resolution Fluorescence Imaging

2012 ◽  
Vol 32 (2) ◽  
pp. 0218001
Author(s):  
于斌 Yu Bin ◽  
陈丹妮 Chen Danni ◽  
刘磊 Liu Lei ◽  
屈军乐 Qu Junle ◽  
牛憨笨 Niu Hanben
Keyword(s):  
Fluorescence Imaging ◽  
Single Molecule ◽  
Super Resolution ◽  
Fourier Domain ◽  
Localization Algorithm ◽  
Nanometer Resolution

scholarly journals Single-molecule diffusometry reveals no catalysis-induced diffusion enhancement of alkaline phosphatase as proposed by FCS experiments

2020 ◽  
Vol 117 (35) ◽  
pp. 21328-21335
Author(s):  
Zhijie Chen ◽  
Alan Shaw ◽  
Hugh Wilson ◽  
Maxime Woringer ◽  
Xavier Darzacq ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Single Molecule ◽  
Particle Tracking ◽  
Single Particle ◽  
Theoretical Models ◽  
Single Particle Tracking ◽  
Correlation Spectroscopy ◽  
Single Molecule Level ◽  
Diffusion Phenomena ◽  
Diffusion Enhancement

Theoretical and experimental observations that catalysis enhances the diffusion of enzymes have generated exciting implications about nanoscale energy flow, molecular chemotaxis, and self-powered nanomachines. However, contradictory claims on the origin, magnitude, and consequence of this phenomenon continue to arise. To date, experimental observations of catalysis-enhanced enzyme diffusion have relied almost exclusively on fluorescence correlation spectroscopy (FCS), a technique that provides only indirect, ensemble-averaged measurements of diffusion behavior. Here, using an anti-Brownian electrokinetic (ABEL) trap and in-solution single-particle tracking, we show that catalysis does not increase the diffusion of alkaline phosphatase (ALP) at the single-molecule level, in sharp contrast to the ∼20% enhancement seen in parallel FCS experiments usingp-nitrophenyl phosphate (pNPP) as substrate. Combining comprehensive FCS controls, ABEL trap, surface-based single-molecule fluorescence, and Monte Carlo simulations, we establish thatpNPP-induced dye blinking at the ∼10-ms timescale is responsible for the apparent diffusion enhancement seen in FCS. Our observations urge a crucial revisit of various experimental findings and theoretical models––including those of our own––in the field, and indicate that in-solution single-particle tracking and ABEL trap are more reliable means to investigate diffusion phenomena at the nanoscale.

Single particle ICP-MS-based absolute and relative quantification ofE. coliO157 16S rRNA using sandwich hybridization capture

The Analyst ◽  
2019 ◽  
Vol 144 (5) ◽  
pp. 1725-1730 ◽  
Author(s):  
Xiaomin Xu ◽  
Jiyun Chen ◽  
Bangrui Li ◽  
Lijuan Tang ◽  
Jianhui Jiang
Keyword(s):  
16S Rrna ◽  
Single Molecule ◽  
Single Particle ◽  
Inductively Coupled Plasma ◽  
Inductively Coupled ◽  
Hybridization Capture ◽  
Sandwich Hybridization ◽  
Icp Ms ◽  
Magnetic Capture ◽  
Plasma Mass Spectrometry

Herein, a novel 16S rRNA detection platform was achieved by combining a sandwich hybridization reaction, a single-molecule magnetic capture, and single particle-inductively coupled plasma mass spectrometry amplification.

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