scholarly journals Microfluidic devices for cell based high throughput screening

Lab on a Chip ◽  
2010 ◽  
Vol 10 (3) ◽  
pp. 341-348 ◽  
Author(s):  
Sarvesh Upadhyaya ◽  
P. Ravi Selvaganapathy
Keyword(s):  
High Throughput ◽  
High Throughput Screening ◽  
Microfluidic Devices

Detection of Respiratory Viruses with Plastic High Throughput Screening Devices

2006 ◽  
Vol 950 ◽  
Author(s):  
Zhengshan Zhao ◽  
Gerardo A. Diaz-Quijada ◽  
Régis Peytavi ◽  
Éric LeBlanc ◽  
Johanne Frenette ◽  
...  
Keyword(s):  
High Throughput ◽  
High Throughput Screening ◽  
Low Cost ◽  
Genetic Material ◽  
Microfluidic Devices ◽  
Medical Diagnostics ◽  
Human Enterovirus ◽  
Plastic Substrate ◽  
Rt Pcr ◽  
Genomics And Proteomics

ABSTRACTMicroarrays have become one of the most convenient tools for high throughput screening and have catalyzed major advances in genomics and proteomics. Other important applications can be found in medical diagnostics, detection of biothreats, drug discovery, etc. Integration of microarrays with microfluidic devices can be highly advantageous in terms of portability, shorter analysis time and lower consumption of expensive biological analytes. Since fabrication of microfluidic devices using traditional materials such as glass is rather expensive, there is a high interest in employing polymeric materials as a low cost alternative suitable for mass production. We present proof-of-concept DNA arrays on a plastic platform for the detection of four important respiratory pathogens: Influenza A virus, respiratory syncytial virus, human enterovirus, and human metapneumovirus.This was accomplished by amplifying the genetic material from the viruses and simultaneously labeling the amplicons with a fluorescent dye (Cy3) via a highly sensitive multiplex Reverse Transcription Polymerase Chain Reaction (RT-PCR). The resultant RT-PCR product was hybridized, without further purification, with an array of specific oligonucleotide probes (20 mers) that had been covalently bound to a plastic substrate. Results indicate a high signal to background ratio that is comparable to commercially available microarray glass slides. In addition, 5 minute hybridization on this plastic substrate has been demonstrated using a centrifugal microfluidic platform, paving the way to a rapid medical diagnostic device for point-of-care use that is based on a low-cost portable Micro-Total-Analysis-System (μ-TAS).

Toward High-Throughput Screening of NAD(P)-Dependent Oxidoreductases Using Boron-Doped Diamond Microelectrodes and Microfluidic Devices

2014 ◽  
Vol 86 (19) ◽  
pp. 9570-9575 ◽  
Author(s):  
Ryo Oyobiki ◽  
Taisuke Kato ◽  
Michinobu Katayama ◽  
Ai Sugitani ◽  
Takeshi Watanabe ◽  
...  
Keyword(s):  
High Throughput ◽  
High Throughput Screening ◽  
Microfluidic Devices ◽  
Boron Doped Diamond ◽  
Boron Doped

Towards Low Cost Disposable High Throughput Screening Devices

2005 ◽  
Vol 897 ◽  
Author(s):  
Gerardo Antonio Diaz-Quijada ◽  
Regis Peytavi ◽  
André Nantel ◽  
Emmanuel Roy ◽  
Michel G. Bergeron ◽  
...  
Keyword(s):  
High Throughput ◽  
High Throughput Screening ◽  
Low Cost ◽  
Polymeric Materials ◽  
Microfluidic Devices ◽  
Medical Diagnostics ◽  
Covalent Immobilization ◽  
Plastic Materials ◽  
Genomics And Proteomics ◽  
Biological Analytes

AbstractMicroarrays have become one of the most convenient tools for high throughput screening, supporting major advances in genomics and proteomics. Other important applications can be found in medical diagnostics, detection of biothreats, drug discovery, etc. Integration of microarrays with microfluidic devices can be highly advantageous in terms of portability, shorter analysis time and lower consumption of expensive biological analytes. Since fabrication of microfluidic devices using traditional materials such as glass is rather expensive, there is a high interest in employing polymeric materials as a low cost alternative that is suitable for mass production. A number of commercially available plastic materials were reviewed for this purpose and poly(methylmethacrylate) and Zeonor™ 1060R were identified as promising candidates, for which methods for surface modification and covalent immobilization of DNA oligonucleotide were developed. In addition, we present proof-of-concept plastic-based microarrays with and without integration with microfluidics.

Microfabrication of single-use plastic microfluidic devices for high-throughput screening and DNA analysis

2002 ◽  
Vol 7 (5-6) ◽  
pp. 265-268 ◽  
Author(s):  
A. Gerlach ◽  
G. Knebel ◽  
A.E. Guber ◽  
M. Heckele ◽  
D. Herrmann ◽  
...  
Keyword(s):  
High Throughput ◽  
High Throughput Screening ◽  
Dna Analysis ◽  
Microfluidic Devices ◽  
Single Use

High-throughput screening program for botanical extracts

Planta Medica ◽  
2012 ◽  
Vol 78 (11) ◽  
Author(s):  
L Hingorani ◽  
NP Seeram ◽  
B Ebersole
Keyword(s):  
High Throughput ◽  
High Throughput Screening ◽  
Screening Program ◽  
Botanical Extracts

High throughput screening of microbial biodiversity for the discovery of novel cosmeceutical agents

Planta Medica ◽  
2015 ◽  
Vol 81 (16) ◽  
Author(s):  
K Georgousaki ◽  
N DePedro ◽  
AM Chinchilla ◽  
N Aliagiannis ◽  
F Vicente ◽  
...  
Keyword(s):  
High Throughput ◽  
High Throughput Screening ◽  
Microbial Biodiversity
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