Development of 2,6-carboxy-substituted boron dipyrromethene (BODIPY) as a novel scaffold of ratiometric fluorescent probes for live cell imaging

2009 ◽  
pp. 7015 ◽  
Author(s):  
Toru Komatsu ◽  
Yasuteru Urano ◽  
Yuuta Fujikawa ◽  
Tomonori Kobayashi ◽  
Hirotatsu Kojima ◽  
...  
Keyword(s):  
Fluorescent Probes ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Boron Dipyrromethene ◽  
Novel Scaffold

scholarly journals “Probe, Sample, and Instrument (PSI)”: The Hat-Trick for Fluorescence Live Cell Imaging

Chemosensors ◽  
2018 ◽  
Vol 6 (3) ◽  
pp. 40 ◽  
Author(s):  
Ludovic Galas ◽  
Thibault Gallavardin ◽  
Magalie Bénard ◽  
Arnaud Lehner ◽  
Damien Schapman ◽  
...  
Keyword(s):  
Organic Synthesis ◽  
Fluorescent Probes ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Cell Labeling ◽  
Key Points ◽  
Research Infrastructures ◽  
New Strategies ◽  
Fluorescence Live Cell Imaging

Cell Imaging Platforms (CIPs) are research infrastructures offering support to a number of scientific projects including the choice of adapted fluorescent probes for live cell imaging. What to detect in what type of sample and for how long is a major issue with fluorescent probes and, for this, the “hat-trick” “Probe–Sample–Instrument” (PSI) has to be considered. We propose here to deal with key points usually discussed in CIPs including the properties of fluorescent organic probes, the modality of cell labeling, and the best equipment to obtain appropriate spectral, spatial, and temporal resolution. New strategies in organic synthesis and click chemistry for accessing probes with enhanced photophysical characteristics and targeting abilities will also be addressed. Finally, methods for image processing will be described to optimize exploitation of fluorescence signals.

Rational design of reversible fluorescent probes for live-cell imaging and quantification of fast glutathione dynamics

2016 ◽  
Vol 9 (3) ◽  
pp. 279-286 ◽  
Author(s):  
Keitaro Umezawa ◽  
Masafumi Yoshida ◽  
Mako Kamiya ◽  
Tatsuya Yamasoba ◽  
Yasuteru Urano
Keyword(s):  
Fluorescent Probes ◽  
Live Cell Imaging ◽  
Rational Design ◽  
Cell Imaging ◽  
Live Cell

Water-soluble BODIPY-conjugated glycopolymers as fluorescent probes for live cell imaging

2013 ◽  
Vol 4 (24) ◽  
pp. 5743 ◽  
Author(s):  
Zhentan Lu ◽  
Lin Mei ◽  
Xinge Zhang ◽  
Yanan Wang ◽  
Yu Zhao ◽  
...  
Keyword(s):  
Fluorescent Probes ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Water Soluble

scholarly journals HaloTag8: an engineered protein tag to improve fluorophore performance

2021 ◽  
Author(s):  
Michelle S. Frei ◽  
Miroslaw Tarnawski ◽  
Julia Roberti ◽  
Birgit Koch ◽  
Julien Hiblot ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
Fluorescent Probes ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Protein Tag

HaloTag8 is an engineered variant of HaloTag7 with up to 40% higher brightness and increased fluorescence lifetime when labeled with fluorogenic rhodamines. Moreover, combining HaloTag8 with HaloTag7 and other fluorescent probes enabled live-cell multiplexing using a single fluorophore and the generation of a fluorescence lifetime-based biosensor. The increased brightness of HaloTag8 and its use in fluorescence lifetime multiplexing makes it a powerful tool for live-cell imaging.

scholarly journals Silafluorene as a promising core for cell-permeant, highly bright and two-photon excitable fluorescent probes for live-cell imaging

2021 ◽  
Vol 187 ◽  
pp. 109083
Author(s):  
Marie Auvray ◽  
Frédéric Bolze ◽  
Gilles Clavier ◽  
Florence Mahuteau-Betzer
Keyword(s):  
Fluorescent Probes ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Two Photon

scholarly journals Development of background-free tame fluorescent probes for intracellular live cell imaging

2016 ◽  
Vol 7 (1) ◽  
Author(s):  
Samira Husen Alamudi ◽  
Rudrakanta Satapathy ◽  
Jihyo Kim ◽  
Dongdong Su ◽  
Haiyan Ren ◽  
...  
Keyword(s):  
Fluorescent Probes ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell

Fluorescent Probes for Live Cell Imaging of Endogenous Guanine Nitration

ChemBioChem ◽  
2013 ◽  
Vol 14 (9) ◽  
pp. 1068-1071 ◽  
Author(s):  
Yohei Saito ◽  
Chiaki Ito ◽  
Shigemoto Fujii ◽  
Tomohiro Sawa ◽  
Takaaki Akaike ◽  
...  
Keyword(s):  
Fluorescent Probes ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell
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