A fast sample preparation procedure for mercury speciation in hair samples by high-performance liquid chromatography coupled to ICP-MS

2010 ◽  
Vol 25 (1) ◽  
pp. 79-83 ◽  
Author(s):  
Samuel S. de Souza ◽  
Jairo L. Rodrigues ◽  
Vanessa C. de Oliveira Souza ◽  
Fernando Barbosa Jr.
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Sample Preparation ◽  
High Performance ◽  
Mercury Speciation ◽  
Preparation Procedure ◽  
Icp Ms ◽  
Hair Samples ◽  
Sample Preparation Procedure

Mercury speciation in whole blood by gas chromatography coupled to ICP-MS with a fast microwave-assisted sample preparation procedure

2011 ◽  
Vol 26 (2) ◽  
pp. 436-442 ◽  
Author(s):  
Jairo L. Rodrigues ◽  
Carolina Rodríguez Alvarez ◽  
Nuria Rodríguez Fariñas ◽  
Juan J. Berzas Nevado ◽  
Fernando Barbosa Jr ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Sample Preparation ◽  
Whole Blood ◽  
Mercury Speciation ◽  
Preparation Procedure ◽  
Microwave Assisted ◽  
Icp Ms ◽  
Sample Preparation Procedure

Testing for Betel Nut Alkaloids in Hair of Papuans Abusers using UPLC–MS/MS and UPLC–Q-Tof-MS

2019 ◽  
Author(s):  
Laurie Gheddar ◽  
François-Xavier Ricaut ◽  
Alice Ameline ◽  
Nicolas Brucato ◽  
Roxanne Tsang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Dietary Habits ◽  
Internal Standard ◽  
Betel Nut ◽  
Chemical Structures ◽  
Hair Samples ◽  
Liquid Chromatography System

Abstract Betel nut is the fruit of Areca palm, growing in Papua New Guinea. Mixed with limestone and stick mustard, arecoline and guvacoline, which are present in betel nut, are hydrolyzed into arecaidine and guvacine, respectively. As part of the study on dietary habits of Papuans residents, our laboratory was asked to analyze the four alkaloids in hair to document long-term exposure. Hair samples were collected from 19 adult subjects (males = 11; females = 8), by some of the authors, and were sent to the laboratory for analysis. The four alkaloids have very similar chemical structures. In order to accurately identify the drugs, two methods were developed. First, the compounds were identified using an ultra-high-performance liquid chromatography system coupled to time-of-flight mass spectrometry. Then, they were quantified by an ultra-high-performance liquid chromatography system coupled to tandem mass spectrometry. After decontamination with dichloromethane, hair samples were cut into very small segments and 20 mg were incubated in methanol for 2 h 30 min in an ultrasound bath. After cooling, the methanol was evaporated to dryness in presence of 20-μL octanol to prevent volatilization. Nicotine-d4 was used as an internal standard. Linearity was observed for concentrations ranging from the limit of quantification to 20 ng/mg for arecoline, arecaidine, guvacine and guvacoline. Measured concentrations were in the range 60 pg/mg to 18 ng/mg for arecoline (n = 19), 14 pg/mg to 2.5 ng/mg for guvacoline (n = 11), 63 pg/mg to 3.8 ng/mg for arecaidine (n = 11) and 100 pg/mg to 3.2 ng/mg for guvacine (n = 6). There was no correlation between concentrations of arecoline and arecaidine (ratio from 0.01 to 0.18) and guvacoline and guvacine (ratio from 0.06 to 3.50). However, the identification of these substances in hair is a good marker of consumption of betel nut and allows us to document a local practice that remains difficult to evaluate just by questioning.

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