Catalytic properties of catechol 1,2-dioxygenase from Acinetobacter radioresistens S13 immobilized on nanosponges

2009 ◽  
pp. 6507 ◽  
Author(s):  
Giovanna Di Nardo ◽  
Carlo Roggero ◽  
Simona Campolongo ◽  
Francesca Valetti ◽  
Francesco Trotta ◽  
...  
Keyword(s):  
Catalytic Properties ◽  
Acinetobacter Radioresistens ◽  
Catechol 1,2 Dioxygenase

The Catechol 1,2 Dioxygenase System of Acinetobacter radioresistens: Isoenzymes, Inductors and Gene Localisation

2001 ◽  
Vol 382 (8) ◽  
Author(s):  
Enrica Pessione ◽  
M. Gabriella Giuffrida ◽  
Roberto Mazzoli ◽  
Patrizia Caposio ◽  
Santo Landolfo ◽  
...  
Keyword(s):  
Acinetobacter Radioresistens ◽  
Catechol 1,2 Dioxygenase ◽  
Gene Localisation

Cloning and characterization of two catechol 1,2-dioxygenase genes from Acinetobacter radioresistens S13

2002 ◽  
Vol 153 (2) ◽  
pp. 69-74 ◽  
Author(s):  
Patrizia Caposio ◽  
Enrica Pessione ◽  
Gabriella Giuffrida ◽  
Amedeo Conti ◽  
Santo Landolfo ◽  
...  
Keyword(s):  
Acinetobacter Radioresistens ◽  
Catechol 1,2 Dioxygenase ◽  
Dioxygenase Genes

Fine-Tuning of Catalytic Properties of Catechol 1,2-Dioxygenase by Active Site Tailoring

ChemBioChem ◽  
2009 ◽  
Vol 10 (6) ◽  
pp. 1015-1024 ◽  
Author(s):  
Raffaella Caglio ◽  
Francesca Valetti ◽  
Patrizia Caposio ◽  
Giorgio Gribaudo ◽  
Enrica Pessione ◽  
...  
Keyword(s):  
Active Site ◽  
Catalytic Properties ◽  
Fine Tuning ◽  
Catechol 1,2 Dioxygenase

An EPR, thermostability and pH-dependence study of wild-type and mutant forms of catechol 1,2-dioxygenase from Acinetobacter radioresistens S13

BioMetals ◽  
2012 ◽  
Vol 26 (1) ◽  
pp. 75-84 ◽  
Author(s):  
Raffaella Caglio ◽  
Enrica Pessione ◽  
Francesca Valetti ◽  
Carlo Giunta ◽  
Elena Ghibaudi
Keyword(s):  
Ph Dependence ◽  
Wild Type ◽  
Acinetobacter Radioresistens ◽  
Catechol 1,2 Dioxygenase ◽  
Mutant Forms

Microstructure and reactivity of small metal particles: The role of Ce additives

1992 ◽  
Vol 50 (1) ◽  
pp. 318-319
Author(s):  
L.D. Schmidt ◽  
K. R. Krause ◽  
J. M. Schwartz ◽  
X. Chu
Keyword(s):  
Atmospheric Pressure ◽  
Noble Metals ◽  
Mixed Oxides ◽  
Catalytic Properties ◽  
Chemical Shifts ◽  
Catalytic Converter ◽  
Structural Evolution ◽  
Single Particles ◽  
Small Metal Particles

The evolution of microstructures of 10- to 100-Å diameter particles of Rh and Pt on SiO2 and Al2O3 following treatment in reducing, oxidizing, and reacting conditions have been characterized by TEM. We are able to transfer particles repeatedly between microscope and a reactor furnace so that the structural evolution of single particles can be examined following treatments in gases at atmospheric pressure. We are especially interested in the role of Ce additives on noble metals such as Pt and Rh. These systems are crucial in the automotive catalytic converter, and rare earths can significantly modify catalytic properties in many reactions. In particular, we are concerned with the oxidation state of Ce and its role in formation of mixed oxides with metals or with the support. For this we employ EELS in TEM, a technique uniquely suited to detect chemical shifts with ∼30Å resolution.

Electron distribution change of small nickel particles encaged in X zeolite in presence of Ce3+ ions, related to their catalytic properties

1986 ◽  
Vol 83 ◽  
pp. 619-621 ◽  
Author(s):  
F. Vergand ◽  
B. Iraqi ◽  
C. Bonnelle ◽  
E. Ramaroson ◽  
M.F. Guilleux ◽  
...  
Keyword(s):  
Electron Distribution ◽  
Catalytic Properties ◽  
Distribution Change ◽  
Nickel Particles ◽  
X Zeolite

On the Preparation of Bovine α-Thrombin

1978 ◽  
Vol 39 (01) ◽  
pp. 193-200 ◽  
Author(s):  
Erwin F Workman ◽  
Roger L Lundblad
Keyword(s):  
Immobilized Enzyme ◽  
Catalytic Properties ◽  
Specific Activity ◽  
Guanidine Hydrochloride ◽  
Low Molecular Weight ◽  
Reversible Process ◽  
Gel Beads ◽  
Tissue Thromboplastin ◽  
C 50 ◽  
Hydrolysis Of

SummaryAn improved method for the preparation of bovine α-thrombin is described. The procedure involves the activation of partially purified prothrombin with tissue thromboplastin followed by chromatography on Sulfopropyl-Sephadex C-50. The purified enzyme is homogeneous on polyacrylamide discontinuous gel electrophoresis and has a specific activity toward fibrinogen of 2,200–2,700 N.I.H. U/mg. Its stability on storage in liquid media is dependent on both ionic strenght and temperature. Increasing ionic strength and decreasing temperature result in optimal stability. The denaturation of α-thrombin by guanidine hydrochloride was found to be a partially reversible process with the renatured species possessing properties similar to “aged” thrombin. In addition, the catalytic properties of a-thrombin covalently attached to agarose gel beads were also examined. The activity of the immobilized enzyme toward fibrinogen was affected to a much greater extent than was the hydrolysis of low molecular weight, synthetic substrates.

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