Effect of silk sericin on assembly of hydroxyapatite nanocrystals into enamel prism-like structure

2009 ◽  
Vol 19 (32) ◽  
pp. 5751 ◽  
Author(s):  
Yurong Cai ◽  
Jun Jin ◽  
Danping Mei ◽  
Nianxin Xia ◽  
Juming Yao
Keyword(s):  
Silk Sericin ◽  
Hydroxyapatite Nanocrystals ◽  
Enamel Prism

scholarly journals Effect of cross-linkable bacterial cellulose nanocrystals on the physicochemical properties of silk sericin films

2021 ◽  
Vol 97 ◽  
pp. 107161
Author(s):  
Jeongmin Nam ◽  
Yujin Hyun ◽  
Subin Oh ◽  
Jinseok Park ◽  
Hyoung-Joon Jin ◽  
...  
Keyword(s):  
Physicochemical Properties ◽  
Bacterial Cellulose ◽  
Cellulose Nanocrystals ◽  
Silk Sericin

Rapid Collagen-Directed Mineralization of Calcium Fluoride Nanocrystals with Periodically Patterned Nanostructure

Nanoscale ◽  
2021 ◽  
Author(s):  
Weijian Fang ◽  
Hang Ping ◽  
Wolfgang Wagermaier ◽  
Shenbao Jin ◽  
Shahrouz Amini ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Calcium Fluoride ◽  
Periodic Structures ◽  
Collagen Fibrils ◽  
Hydroxyapatite Nanocrystals

Collagen fibrils present periodic structures, which provide space for intrafibrillar growth of oriented hydroxyapatite nanocrystals in bone and contribute to the good mechanical properties of bone. However, there are not...

scholarly journals Green synthesis of silk sericin-embedded silver nanoparticles and their antibacterial application against multidrug-resistant pathogens

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Md. Abdullah Al Masud ◽  
Hamid Shaikh ◽  
Md. Shamsul Alam ◽  
M. Minnatul Karim ◽  
M. Abdul Momin ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Bombyx Mori ◽  
Multidrug Resistant ◽  
Visible Spectroscopy ◽  
Silk Sericin ◽  
X Ray ◽  
Synthesis Strategy ◽  
Uv Visible ◽  
Multidrug Resistant Pathogens

Abstract Background The green synthesis strategy of metallic nanoparticles (NPs) has become popular due to being environmentally friendly. Stable silver nanoparticles (AgNPs) have been synthesized by natural products such as starch, soy protein, various extract of leaves, barks, and roots functioning both as reducing and stabilizing agents. Likewise, silk sericin (SS) is a globular protein discarded in the silk factory might be used for NP synthesis. In this research, we focus on the green synthesis and stabilization of AgNPs by SS as well as assessment of their antibacterial activities against some drug-resistant pathogen. Results SS was extracted from Bombyx mori silkworm cocoons in an aqueous medium. 17 w/w% of dry sericin powder with respect to the cocoon’s weight was obtained by freeze-drying. Furthermore, AgNPs conjugated to sericin, i.e., SS-capped silver nanoparticles (SS-AgNPs) were synthesized by easy, cost-effective, and environment-friendly methods. The synthesized SS-AgNPs were characterized by UV-visible spectroscopy, Fourier-transform infrared-attenuated total reflection (FTIR-ATR) spectroscopy, transmission electron microscopy (TEM), and X-ray diffraction measurement. It has been found from the absorbance of UV-visible spectroscopy that a higher percent of SS-AgNPs was obtained at a higher concentration of silver nitrate solution. FTIR-ATR spectra showed that the carboxylate groups obtained from silk sericin act as a reducing agent for the synthesis of silver nanoparticles, while NH2+ and COO− act as a stabilizer of AgNPs. The X-ray diffractogram of SS-AgNPs was quite different from AgNO3 and sericin due to a change in the crystal structure. The diameter of AgNPs was around 20–70 nm observed using TEM. The synthesized SS-AgNPs exhibited strong antibacterial activity against multidrug-resistant pathogens, Escherichia coli and Pseudomonas aeruginosa. Minimal inhibitory/bactericidal concentrations against E. coli and P. aeruginosa were 20μg/mL. Conclusions This study encourages the use of Bombyx mori for the ecofriendly synthesis of SS-AgNPs to control multidrug-resistant microorganisms.

scholarly journals Mechanical Enhancement of Cytocompatible 3D Scaffolds, Consisting of Hydroxyapatite Nanocrystals and Natural Biomolecules, Through Physical Cross-Linking

2020 ◽  
Vol 7 (3) ◽  
pp. 96
Author(s):  
Despoina Brasinika ◽  
Elias P. Koumoulos ◽  
Kyriaki Kyriakidou ◽  
Eleni Gkartzou ◽  
Maria Kritikou ◽  
...  
Keyword(s):  
Elastic Modulus ◽  
Cell Attachment ◽  
Great Promise ◽  
Porous Network ◽  
Mechanical Reinforcement ◽  
Experimental Conditions ◽  
Tissue Properties ◽  
Human Osteosarcoma Cells ◽  
Hydroxyapatite Nanocrystals ◽  
Physical Crosslinking

Bioinspired scaffolds mimicking natural bone-tissue properties holds great promise in tissue engineering applications towards bone regeneration. Within this work, a way to reinforce mechanical behavior of bioinspired bone scaffolds was examined by applying a physical crosslinking method. Scaffolds consisted of hydroxyapatite nanocrystals, biomimetically synthesized in the presence of collagen and l-arginine. Scaffolds were characterized by X-ray diffraction, Fourier transform infrared spectroscopy, scanning electron microscopy (SEM), microcomputed tomography, and nanoindentation. Results revealed scaffolds with bone-like nanostructure and composition, thus an inherent enhanced cytocompatibility. Evaluation of porosity proved the development of interconnected porous network with bimodal pore size distribution. Mechanical reinforcement was achieved through physical crosslinking with riboflavin irradiation, and nanoindentation tests indicated that within the experimental conditions of 45% humidity and 37 °C, photo-crosslinking led to an increase in the scaffold’s mechanical properties. Elastic modulus and hardness were augmented, and specifically elastic modulus values were doubled, approaching equivalent values of trabecular bone. Cytocompatibility of the scaffolds was assessed using MG63 human osteosarcoma cells. Cell viability was evaluated by double staining and MTT assay, while attachment and morphology were investigated by SEM. The results suggested that scaffolds provided a cell friendly environment with high levels of viability, thus supporting cell attachment, spreading and proliferation.

scholarly journals Preparation of Silk Sericin/Lignin Blend Beads for the Removal of Hexavalent Chromium Ions

2016 ◽  
Vol 17 (9) ◽  
pp. 1466 ◽  
Author(s):  
Hyo Kwak ◽  
Munju Shin ◽  
Haesung Yun ◽  
Ki Lee
Keyword(s):  
Hexavalent Chromium ◽  
Silk Sericin ◽  
Chromium Ions

Preparation of self-assembled silk sericin nanoparticles

2003 ◽  
Vol 32 (1-2) ◽  
pp. 36-42 ◽  
Author(s):  
Kwang Yong Cho ◽  
Jae Yu Moon ◽  
Yong Woo Lee ◽  
Kwang Gill Lee ◽  
Joo Hong Yeo ◽  
...  
Keyword(s):  
Silk Sericin ◽  
Self Assembled
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