Advancing high-throughput gene synthesis technology

2009 ◽  
Vol 5 (7) ◽  
pp. 714 ◽  
Author(s):  
Jingdong Tian ◽  
Kuosheng Ma ◽  
Ishtiaq Saaem
Keyword(s):  
High Throughput ◽  
Gene Synthesis

scholarly journals A set of experimentally validated, mutually orthogonal primers for combinatorially specifying genetic components

2018 ◽  
Vol 3 (1) ◽  
Author(s):  
Subu K Subramanian ◽  
William P Russ ◽  
Rama Ranganathan
Keyword(s):  
Synthetic Biology ◽  
High Throughput ◽  
Dna Sequences ◽  
Gene Synthesis ◽  
Modular Architecture ◽  
Design And Synthesis ◽  
Genetic Components ◽  
Polymerase Chain ◽  
Polymerase Chain Reaction Primers ◽  
Microarray Chips

Abstract The design and synthesis of novel genes and deoxyribonucleic acid (DNA) sequences is a central technique in synthetic biology. Current methods of high throughput gene synthesis use pooled oligonucleotides obtained from custom-designed DNA microarray chips, and rely on orthogonal (non-interacting) polymerase chain reaction primers to specifically de-multiplex, by amplification, the precise subset of oligonucleotides necessary to assemble a full length gene. The availability of a large validated set of mutually orthogonal primers is therefore a crucial reagent for high-throughput gene synthesis. Here, we present a set of 166 20-nucleotide primers that are experimentally verified to be non-interacting, capable of specifying 13 695 unique genes. These primers represent a valuable resource to the synthetic biology community for specifying genetic components that can be assembled through a scalable and modular architecture.

A Novel Platform for High-Throughput Gene Synthesis to Maximize Recombinant Expression in Escherichia coli

2017 ◽  
pp. 113-128 ◽  
Author(s):  
Ana Filipa Sequeira ◽  
Joana L. A. Brás ◽  
Vânia O. Fernandes ◽  
Catarina I. P. D. Guerreiro ◽  
Renaud Vincentelli ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
High Throughput ◽  
Recombinant Expression ◽  
Gene Synthesis ◽  
Expression In Escherichia Coli

scholarly journals High-fidelity gene synthesis by retrieval of sequence-verified DNA identified using high-throughput pyrosequencing

2010 ◽  
Vol 28 (12) ◽  
pp. 1291-1294 ◽  
Author(s):  
Mark Matzas ◽  
Peer F Stähler ◽  
Nathalie Kefer ◽  
Nicole Siebelt ◽  
Valesca Boisguérin ◽  
...  
Keyword(s):  
High Throughput ◽  
Gene Synthesis ◽  
High Fidelity

scholarly journals A novel solid phase technology for high-throughput gene synthesis

BioTechniques ◽  
2008 ◽  
Vol 45 (3) ◽  
pp. 340-343 ◽  
Author(s):  
Jan Van den Brulle ◽  
Markus Fischer ◽  
Thomas Langmann ◽  
Gudrun Horn ◽  
Thomas Waldmann ◽  
...  
Keyword(s):  
High Throughput ◽  
Solid Phase ◽  
Gene Synthesis

154: Integration of TPSA and High-Throughput Mass Spectrometry Data Improves Prostate Cancer Prediction

2007 ◽  
Vol 177 (4S) ◽  
pp. 52-53
Author(s):  
Stefano Ongarello ◽  
Eberhard Steiner ◽  
Regina Achleitner ◽  
Isabel Feuerstein ◽  
Birgit Stenzel ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Mass Spectrometry ◽  
High Throughput ◽  
Mass Spectrometry Data ◽  
Cancer Prediction
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