A protein-supported fluorescent reagent for the highly-sensitive and selective detection of mercury ions in aqueous solution and live cells

2008 ◽  
pp. 6345 ◽  
Author(s):  
Li-Jun Ma ◽  
Yue Li ◽  
Lei Li ◽  
Jian Sun ◽  
Chunjuan Tian ◽  
...  
2017 ◽  
Vol 196 ◽  
pp. 377-393 ◽  
Author(s):  
Danni Liu ◽  
Shenglu Ji ◽  
Heran Li ◽  
Liang Hong ◽  
Deling Kong ◽  
...  

The exploration of advanced fluorescent probes that can detect divalent copper (Cu2+) in aqueous environments and even in live organisms is particularly valuable for understanding the occurrence and development of Cu2+-related diseases. In this work, we report the design and synthesis of an aggregation-induced emission luminogen (AIEgen)-based probe (TPE-Py-EEGTIGYG) by integrating an AIEgen, TPE-Py, with a peptide, EEGTIGYG, which can selectively detect Cu2+ in both aqueous solution and live cells. Peptide EEGTIGYG has dual functionality in the probe design, namely improving water solubility and providing specific cell membrane-binding ability. TPE-Py-EEGTIGYG can self-assemble into nanoaggregates at high concentration in aqueous solution (e.g., 25 μM), which possess large fluorescence output due to the restriction of intramolecular rotation of the phenyl rings on TPE-Py. The fluorescence of the TPE-Py-EEGTIGYG nanoaggregates can be significantly quenched by Cu2+ but not by other metal ions, achieving the selective detection of Cu2+ in aqueous media. Furthermore, TPE-Py-EEGTIGYG can exist as a molecular species and is very weakly fluorescent in dilute aqueous solution (e.g., 5 μM), but can however largely switch on its fluorescence upon specifically anchoring onto the cell membrane. The emissive probes on the cell membrane can be used for the detection of Cu2+ ions that move in and out of cells with a fluorescence “turn-off” mode.


RSC Advances ◽  
2015 ◽  
Vol 5 (95) ◽  
pp. 77755-77759 ◽  
Author(s):  
Bo Yang ◽  
Xiaochun Chen ◽  
Renyong Liu ◽  
Bianhua Liu ◽  
Changlong Jiang

A highly sensitive and selective detection of As(iii) was reported by target induced aggregation of nanoparticles enhanced Raman spectroscopic technique.


The Analyst ◽  
2013 ◽  
Vol 138 (7) ◽  
pp. 2057 ◽  
Author(s):  
Qing Yang ◽  
Fan Li ◽  
Yan Huang ◽  
Hui Xu ◽  
Linsheng Tang ◽  
...  

RSC Advances ◽  
2015 ◽  
Vol 5 (120) ◽  
pp. 99099-99106 ◽  
Author(s):  
Shaobo Cui ◽  
Sai Xu ◽  
Hongwei Song ◽  
Wen Xu ◽  
Xu Chen ◽  
...  

The detection of Hg2+ has attracted considerable attention because of the serious health and environmental problems caused by it.


2018 ◽  
Vol 8 (12) ◽  
pp. 1719-1724
Author(s):  
Kunjie Wang ◽  
Qiuyun Shi ◽  
Qingjuan Ji ◽  
Hongxia Li ◽  
Deyi Zhang ◽  
...  

2013 ◽  
Vol 42 (5) ◽  
pp. 1827-1833 ◽  
Author(s):  
Wei Wang ◽  
Qian Wen ◽  
Yue Zhang ◽  
Xiaoliang Fei ◽  
Yaoxian Li ◽  
...  

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