scholarly journals Modular tissue engineering: engineering biological tissues from the bottom up

Soft Matter ◽  
2009 ◽  
Vol 5 (7) ◽  
pp. 1312 ◽  
Author(s):  
Jason W. Nichol ◽  
Ali Khademhosseini
Keyword(s):  
Tissue Engineering ◽  
Biological Tissues ◽  
Bottom Up ◽  
Modular Tissue Engineering

scholarly journals Engineering Biological Tissues from the Bottom-Up: Recent Advances and Future Prospects

Micromachines ◽  
2021 ◽  
Vol 13 (1) ◽  
pp. 75
Author(s):  
Xiaowen Wang ◽  
Zhen Wang ◽  
Wenya Zhai ◽  
Fengyun Wang ◽  
Zhixing Ge ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Future Development ◽  
Biological Tissues ◽  
Future Prospects ◽  
Top Down ◽  
Bottom Up ◽  
Nano Technology ◽  
Critical Overview ◽  
Development Prospects

Tissue engineering provides a powerful solution for current organ shortages, and researchers have cultured blood vessels, heart tissues, and bone tissues in vitro. However, traditional top-down tissue engineering has suffered two challenges: vascularization and reconfigurability of functional units. With the continuous development of micro-nano technology and biomaterial technology, bottom-up tissue engineering as a promising approach for organ and tissue modular reconstruction has gradually developed. In this article, relevant advances in living blocks fabrication and assembly techniques for creation of higher-order bioarchitectures are described. After a critical overview of this technology, a discussion of practical challenges is provided, and future development prospects are proposed.

scholarly journals Polymeric Microspheres/Cells/Extracellular Matrix Constructs Produced by Auto-Assembly for Bone Modular Tissue Engineering

2021 ◽  
Vol 22 (15) ◽  
pp. 7897
Author(s):  
Bartosz Mielan ◽  
Daniela M. Sousa ◽  
Małgorzata Krok-Borkowicz ◽  
Pierre Eloy ◽  
Christine Dupont ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cell Culture ◽  
Extracellular Matrix ◽  
Osteogenic Differentiation ◽  
Photoelectron Spectroscopy ◽  
Building Blocks ◽  
Biological Tissues ◽  
Type I ◽  
Experimental Conditions ◽  
Modular Tissue Engineering

Modular tissue engineering (MTE) is a novel “bottom-up” approach to create engineered biological tissues from microscale repeating units. Our aim was to obtain microtissue constructs, based on polymer microspheres (MSs) populated with cells, which can be further assembled into larger tissue blocks and used in bone MTE. Poly(L-lactide-co-glycolide) MS of 165 ± 47 µm in diameter were produced by oil-in-water emulsification and treated with 0.1 M NaOH. To improve cell adhesion, MSs were coated with poly-L-lysine (PLL) or human recombinant collagen type I (COL). The presence of oxygenated functionalities and PLL/COL coating on MS was confirmed by X-ray photoelectron spectroscopy (XPS). To assess the influence of medium composition on adhesion, proliferation, and osteogenic differentiation, preosteoblast MC3T3-E1 cells were cultured on MS in minimal essential medium (MEM) and osteogenic differentiation medium (OSG). Moreover, to assess the potential osteoblast–osteoclast cross-talk phenomenon and the influence of signaling molecules released by osteoclasts on osteoblast cell culture, a medium obtained from osteoclast culture (OSC) was also used. To impel the cells to adhere and grow on the MS, anti-adhesive cell culture plates were utilized. The results show that MS coated with PLL and COL significantly favor the adhesion and growth of MC3T3-E1 cells on days 1 and 7, respectively, in all experimental conditions tested. On day 7, three-dimensional MS/cell/extracellular matrix constructs were created owing to auto-assembly. The cells grown in such constructs exhibited high activity of early osteogenic differentiation marker, namely, alkaline phosphatase. Superior cell growth on PLL- and COL-coated MS on day 14 was observed in the OSG medium. Interestingly, deposition of extracellular matrix and its mineralization was particularly enhanced on COL-coated MS in OSG medium on day 14. In our study, we developed a method of spontaneous formation of organoid-like MS-based cell/ECM constructs with a few millimeters in size. Such constructs may be regarded as building blocks in bone MTE.

scholarly journals Protein Hydrogels: The Swiss Army Knife for Enhanced Mechanical and Bioactive Properties of Biomaterials

Nanomaterials ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 1656
Author(s):  
Carla Huerta-López ◽  
Jorge Alegre-Cebollada
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Polymer Networks ◽  
Biological Tissues ◽  
Modern Medicine ◽  
Body Parts ◽  
Biomedical Sciences ◽  
Protein Hydrogels ◽  
Large Water ◽  
Design Options

Biomaterials are dynamic tools with many applications: from the primitive use of bone and wood in the replacement of lost limbs and body parts, to the refined involvement of smart and responsive biomaterials in modern medicine and biomedical sciences. Hydrogels constitute a subtype of biomaterials built from water-swollen polymer networks. Their large water content and soft mechanical properties are highly similar to most biological tissues, making them ideal for tissue engineering and biomedical applications. The mechanical properties of hydrogels and their modulation have attracted a lot of attention from the field of mechanobiology. Protein-based hydrogels are becoming increasingly attractive due to their endless design options and array of functionalities, as well as their responsiveness to stimuli. Furthermore, just like the extracellular matrix, they are inherently viscoelastic in part due to mechanical unfolding/refolding transitions of folded protein domains. This review summarizes different natural and engineered protein hydrogels focusing on different strategies followed to modulate their mechanical properties. Applications of mechanically tunable protein-based hydrogels in drug delivery, tissue engineering and mechanobiology are discussed.

scholarly journals Bottom-up tissue engineering

2011 ◽  
Vol 22 (5) ◽  
pp. 674-680 ◽  
Author(s):  
Donald L Elbert
Keyword(s):  
Tissue Engineering ◽  
Bottom Up

Cell-laden microfibers for bottom-up tissue engineering

2015 ◽  
Vol 20 (2) ◽  
pp. 236-246 ◽  
Author(s):  
Hiroaki Onoe ◽  
Shoji Takeuchi
Keyword(s):  
Tissue Engineering ◽  
Bottom Up

scholarly journals Computer modelling and simulation of a novel printing head for complex tissue engineering constructs

2020 ◽  
Vol 318 ◽  
pp. 01045
Author(s):  
Gokhan Ates
Keyword(s):  
Tissue Engineering ◽  
Computer Modelling ◽  
Three Dimensional ◽  
Biological Tissues ◽  
Biological Properties ◽  
Functionally Graded ◽  
Mixing Efficiency ◽  
Tissue Constructs ◽  
Multiple Materials ◽  
Graded Structures

In tissue engineering, three-dimensional functional scaffolds with tailored biological properties are needed to be able to mimic the hierarchical structure of biological tissues. Recent developments in additive biomanufacturing allow to extrude multiple materials enabling the fabrication of more sophisticated tissue constructs. These multi-material biomanufacturing systems comprise multiple printing heads through which individual materials are sequentially printed. Nevertheless, as more printing heads are added the fabrication process significantly decreases, since it requires mechanical switching among the physically separated printheads to enable printing multiple materials. In addition, this approach is not able to create biomimetic tissue constructs with property gradients. To address these limitations, this paper presents a novel static mixing extrusion printing head to enable the fabrication of multi-material, functionally graded structures using a single nozzle. Computational fluid dynamics (CFD) was used to numerically analyze the influence of Reynolds number on the flow pattern of biomaterials and mixing efficiency considering different miscible materials.

DEVELOPMENTAL STATUS AND PERSPECTIVES FOR TISSUE ENGINEERING IN UROLOGY

2021 ◽  
Vol 12 (07) ◽  
pp. 5-13
Author(s):  
Elcin Nizami Huseyn ◽  
Keyword(s):  
Tissue Engineering ◽  
Biological Materials ◽  
Organ Damage ◽  
Biological Tissues ◽  
Tissue Cell ◽  
Sperm Cells ◽  
Engineering Technology ◽  
The Future ◽  
Tissue Engineering Technology

Tissue engineering technology and tissue cell-based stem cell research have made great strides in treating tissue and organ damage, correcting tissue and organ dysfunction, and reducing surgical complications. In the past, traditional methods have used biological substitutes for tissue repair materials, while tissue engineering technology has focused on merging sperm cells with biological materials to form biological tissues with the same structure and function as their own tissues. The advantage is that tissue engineering technology can overcome donors. Material procurement restrictions can effectively reduce complications. The aim of studying tissue engineering technology is to find sperm cells and suitable biological materials to replace the original biological functions of tissues and to establish a suitable in vivo microenvironment. This article mainly describes the current developments of tissue engineering in various fields of urology and discusses the future trends of tissue engineering technology in the treatment of complex diseases of the urinary system. The results of the research in this article indicate that while the current clinical studies are relatively few, the good results from existing animal model studies indicate good prospects of tissue engineering technology for the treatment of various urinary tract diseases in the future. Key words: Tissue engineering, kidney, ureter, bladder, urethra

scholarly journals Blue Light Switchable Cell–Cell Interactions Provide Reversible and Spatiotemporal Control Towards Bottom‐Up Tissue Engineering

2019 ◽  
Vol 3 (4) ◽  
pp. 1800310 ◽  
Author(s):  
Simge G. Yüz ◽  
Samaneh Rasoulinejad ◽  
Marc Mueller ◽  
Anatol E. Wegner ◽  
Seraphine V. Wegner
Keyword(s):  
Tissue Engineering ◽  
Blue Light ◽  
Cell Interactions ◽  
Bottom Up ◽  
Spatiotemporal Control ◽  
Cell Cell

Tissue Engineering with Natural Tissue Matrices

2010 ◽  
Vol 76 ◽  
pp. 125-132 ◽  
Author(s):  
Akio Kishida ◽  
Seiichi Funamoto ◽  
Jun Negishi ◽  
Yoshihide Hashimoto ◽  
Kwangoo Nam ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Blood Vessel ◽  
Heart Valve ◽  
Biomechanical Properties ◽  
Biological Tissues ◽  
The Novel ◽  
Detergent Treatment ◽  
Decellularized Tissue ◽  
Natural Tissue ◽  
Porcine Tissues

Natural tissue, especially autologous tissue is one of ideal materials for tissue regeneration. Decellularized tissue could be assumed as a second choice because the structure and the mechanical properties are well maintained. Decellularized human tissues, for instance, heart valve, blood vessel, and corium, have already been developed and applied clinically. Nowadays, decellularized porcine tissues are also investigated. These decellularized tissues were prepared by detergent treatment. The detergent washing is easy but sometime it has problems. We have developed the novel decellularization method, which applied the high-hydrostatic pressure (HHP). As the tissue set in the pressurizing chamber is treated uniformly, the effect of the high-hydrostatic pressurization does not depend on the size of tissue. We have reported the HHP decellularization of heart valve, blood vessel, bone, and cornea. Furthermore, HHP treatments are reported to have the ability of the extinction of bacillus and the inactivation of virus. So, the HHP treatment is also expected as the sterilization method. We are investigating efficient processes of decellularization and recellularization of biological tissues to have bioscaffolds keeping intact structure and biomechanical properties. Our recent studies on tissue engineering using HHP decellularized tissue will be reported here.

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