Characterisation of porous freeze dried conducting carbon nanotube–chitosan scaffolds

2008 ◽  
Vol 18 (44) ◽  
pp. 5417 ◽  
Author(s):  
Luke Joshua Sweetman ◽  
Simon Edward Moulton ◽  
Gordon George Wallace
Keyword(s):  
Carbon Nanotube ◽  
Freeze Dried ◽  
Chitosan Scaffolds

scholarly journals Mutual influence of selenium nanoparticles and FGF2-STAB® on biocompatible properties of collagen/chitosan 3D scaffolds: in vitro and ex ovo evaluation

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Johana Muchová ◽  
Vanessa Hearnden ◽  
Lenka Michlovská ◽  
Lucie Vištejnová ◽  
Anna Zavaďáková ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Metabolic Activity ◽  
Mutual Influence ◽  
Chorioallantoic Membrane ◽  
Antagonistic Effect ◽  
Dermal Fibroblasts ◽  
Selenium Nanoparticles ◽  
Freeze Dried ◽  
Chitosan Scaffolds

AbstractIn a biological system, nanoparticles (NPs) may interact with biomolecules. Specifically, the adsorption of proteins on the nanoparticle surface may influence both the nanoparticles' and proteins' overall bio-reactivity. Nevertheless, our knowledge of the biocompatibility and risk of exposure to nanomaterials is limited. Here, in vitro and ex ovo biocompatibility of naturally based crosslinked freeze-dried 3D porous collagen/chitosan scaffolds, modified with thermostable fibroblast growth factor 2 (FGF2-STAB®), to enhance healing and selenium nanoparticles (SeNPs) to provide antibacterial activity, were evaluated. Biocompatibility and cytotoxicity were tested in vitro using normal human dermal fibroblasts (NHDF) with scaffolds and SeNPs and FGF2-STAB® solutions. Metabolic activity assays indicated an antagonistic effect of SeNPs and FGF2-STAB® at high concentrations of SeNPs. The half-maximal inhibitory concentration (IC50) of SeNPs for NHDF was 18.9 µg/ml and IC80 was 5.6 µg/ml. The angiogenic properties of the scaffolds were monitored ex ovo using a chick chorioallantoic membrane (CAM) assay and the cytotoxicity of SeNPs over IC80 value was confirmed. Furthermore, the positive effect of FGF2-STAB® at very low concentrations (0.01 µg/ml) on NHDF metabolic activity was observed. Based on detailed in vitro testing, the optimal concentrations of additives in the scaffolds were determined, specifically 1 µg/ml of FGF2-STAB® and 1 µg/ml of SeNPs. The scaffolds were further subjected to antimicrobial tests, where an increase in selenium concentration in the collagen/chitosan scaffolds increased the antibacterial activity. This work highlights the antimicrobial ability and biocompatibility of newly developed crosslinked collagen/chitosan scaffolds involving FGF2-STAB® and SeNPs. Moreover, we suggest that these sponges could be used as scaffolds for growing cells in systems with low mechanical loading in tissue engineering, especially in dermis replacement, where neovascularization is a crucial parameter for successful skin regeneration. Due to their antimicrobial properties, these scaffolds are also highly promising for tissue replacement requiring the prevention of infection.

scholarly journals Freeze-Dried Carbon Nanotube Aerogels for High-Frequency Absorber Applications

2018 ◽  
Vol 10 (23) ◽  
pp. 19806-19811 ◽  
Author(s):  
Ilya V. Anoshkin ◽  
James Campion ◽  
Dmitri V. Lioubtchenko ◽  
Joachim Oberhammer
Keyword(s):  
Carbon Nanotube ◽  
High Frequency ◽  
Freeze Dried

A comprehensive in vitro study of the carbon nanotube enhanced chitosan scaffolds for cancellous bone regeneration

2018 ◽  
Vol 4 (3) ◽  
pp. 035027 ◽  
Author(s):  
Mohammad Hassan Ramezan Zadeh ◽  
Majid Seifi ◽  
Maryam Abdolrahimi ◽  
Mahvash Hadavi
Keyword(s):  
Carbon Nanotube ◽  
Bone Regeneration ◽  
Cancellous Bone ◽  
In Vitro Study ◽  
Vitro Study ◽  
Chitosan Scaffolds

scholarly journals Hydroxyapatite-Based Magnetic Bionanocomposite as Pharmaceuticals Carriers in Chitosan Scaffolds

2021 ◽  
Vol 5 (2) ◽  
pp. 37
Author(s):  
Anderson Valério Chaves ◽  
Rafael Melo Freire ◽  
Victor Pinheiro Feitosa ◽  
Nágila Maria Pontes Silva Ricardo ◽  
Juliano Casagrande Denardin ◽  
...  
Keyword(s):  
Bone Growth ◽  
Average Particle Size ◽  
Electronic Spectroscopy ◽  
Average Particle ◽  
High Porosity ◽  
Magnetite Fe3o4 ◽  
Freeze Dried ◽  
Chitosan Scaffolds ◽  
Co Precipitation ◽  
New System

Hydroxyapatite (HA) is a bioceramic very similar to the mineral component of bones and teeth. It is well established that osteoblasts grow better onto HA-coated metals than on metals alone. Herein, the preparation of a new system consisting of magnetite (Fe3O4) and HA functionalized with oleic acid and simvastatin (SIMV), and incorporated in chitosan (CHI) scaffolds, was undertaken. HA was synthesized by the hydrothermal method, while Fe3O4 was synthesized by co-precipitation. The polymer matrix was obtained using a 2% CHI solution, and allowed to stir for 2 h. The final material was freeze-dried to produce scaffolds. The magnetic properties remained unchanged after the formation of the composite, as well as after the preparation of the scaffolds, maintaining the superparamagnetism. CHI scaffolds were analyzed by scanning electronic spectroscopy (SEM) and showed a high porosity, with very evident cavities, which provides the functionality of bone growth support during the remineralization process in possible regions affected by bone tissue losses. The synthesized composite showed an average particle size between 15 and 23 nm for particles (HA and Fe3O4). The scaffolds showed considerable porosity, which is important for the performance of various functions of the tissue structure. Moreover, the addition of simvastatin in the system can promote bone formation.

Fabrication, Surface Modification and Analysis of Biocompatibility of Biologic Chitosan Scaffold

2008 ◽  
Vol 373-374 ◽  
pp. 654-657 ◽  
Author(s):  
Ke Dong Song ◽  
Peng Fei Wen ◽  
Tian Qing Liu ◽  
Li Li Jiang ◽  
Guan Yu Mei
Keyword(s):  
Surface Modification ◽  
Film Coating ◽  
Biological Properties ◽  
Chitosan Scaffold ◽  
Freeze Dried ◽  
Cell Scaffold ◽  
Marrow Mesenchymal Stem Cells ◽  
Culture Process ◽  
Chitosan Scaffolds ◽  
Rat Tail

In this study, the surface modification and biocompatibility of the biologic chitosan scaffold were investigated. The chitosan scaffold with excellent reticular structure was attained after being purified, emulsionized, cross-linked, molded and freeze-dried step by step by using the native materials, coming from such as lobster shell, crab shell etc.. After that, its surface modification was operated with film coating by using gelatin. Then the bone marrow mesenchymal stem cells (BMSCs) derived from New Zealand rabbits were used as the seed cells and were inoculated onto the modified biologic chitosan scaffolds at 3×105 cells/ml to investigate the biocompatibility and bone conductive efficiency of this kind of scaffold in static culture for one week. As a control, the cell suspensions with same densities were inoculated onto the chitosan scaffold without being treated. During the whole culture process, the cellular adherence and expansion were observed under inverted microscope. After culture, the biological properties of the fabricated cell-scaffold tissues were detected by scanning electron microscope (SEM) and HO/PI fluorescent double staining. The results showed that the biologic chitosan scaffold treated with gelatin or rat-tail collagen promoted a higher adhesion and proliferation of BMSCs in comparison to the untreated samples. Besides, the BMSCs within the treated scaffold were more regular and well-distributed than those in untreated one. It is concluded that this kind of surface modification can be used to change the physicochemical properties of chitosan scaffold. The improved biologic chitosan scaffold is suitable to be an ideal biomedical scaffold for tissue engineering.

scholarly journals Influence of freezing temperature and deacetylation degree on the performance of freeze-dried chitosan scaffolds towards cartilage tissue engineering

2017 ◽  
Vol 95 ◽  
pp. 232-240 ◽  
Author(s):  
Lara Lopes Reys ◽  
Simone Santos Silva ◽  
Rogério Pedro Pirraco ◽  
Alexandra Pinto Marques ◽  
João Filipe Mano ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cartilage Tissue Engineering ◽  
Freezing Temperature ◽  
Cartilage Tissue ◽  
Deacetylation Degree ◽  
Freeze Dried ◽  
Chitosan Scaffolds

scholarly journals Freeze-Dried Sulfur–Graphene Oxide–Carbon Nanotube Nanocomposite for High Sulfur-Loading Lithium/Sulfur Cells

Nano Letters ◽  
2017 ◽  
Vol 17 (11) ◽  
pp. 7086-7094 ◽  
Author(s):  
Yoon Hwa ◽  
Hyeon Kook Seo ◽  
Jong-min Yuk ◽  
Elton J. Cairns
Keyword(s):  
Graphene Oxide ◽  
Carbon Nanotube ◽  
Freeze Dried ◽  
Lithium Sulfur ◽  
High Sulfur Loading
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