Immobilised metal affinity chromatography for the capture of hydroxamate-containing siderophores and other Fe(iii)-binding metabolites directly from bacterial culture supernatants

The Analyst ◽  
2008 ◽  
Vol 133 (7) ◽  
pp. 877 ◽  
Author(s):  
Najwa Braich ◽  
Rachel Codd
Keyword(s):  
Affinity Chromatography ◽  
Bacterial Culture ◽  
Metal Affinity ◽  
Culture Supernatants ◽  
Immobilised Metal Affinity Chromatography

Immobilised Metal Affinity Chromatography for the Analysis of Proteins and Peptides

2008 ◽  
Vol 4 (2) ◽  
pp. 127-135 ◽  
Author(s):  
Gushinder Kaur-Atwal ◽  
Daniel Weston ◽  
Philip Bonner ◽  
Susan Crosland ◽  
Philip Green ◽  
...  
Keyword(s):  
Affinity Chromatography ◽  
Metal Affinity ◽  
Immobilised Metal Affinity Chromatography ◽  
Proteins And Peptides

scholarly journals Trace elements species fractionation in rye flour and rye (Secale cereale L.) seedlings

2009 ◽  
Vol 27 (No. 1) ◽  
pp. 39-48
Author(s):  
J. Polák ◽  
O. Mestek ◽  
R. Koplík ◽  
J. Šantrůček ◽  
J. Komínková ◽  
...  
Keyword(s):  
Trace Elements ◽  
Affinity Chromatography ◽  
Secale Cereale ◽  
Fresh Matter ◽  
Physiological Level ◽  
Metal Affinity ◽  
Rye Flour ◽  
Icp Ms ◽  
Minor Part ◽  
Immobilised Metal Affinity Chromatography

The fractionation of Cd, Cu, Mo, Ni, and Zn species in extracts of rye (cv. Fernando) seedlings (grown up in both standard and Cd<sup>2+</sup>-enriched medium) and rye flour was performed by SEC/ICP−MS method. The majority of Cu, Zn, and Ni in all samples were bound in the 1–2 kDa fraction. Molybdenum occurred in all samples in the fraction of 3 kDa. During five days of cultivation in a solution of 30 µmol/l Cd<sup>2+</sup>, the plants accumulated as much as 5 mg/kg fresh matter of Cd, but its soluble portion represented only 12–15%. The prevailing portion of Cd complexes was contained in the fraction of 3 kDa, while the minor part occurred in the fraction of 20 kDa. The speciation of elevated Cd in plants differs from that of other metals present at a physiological level. The metal-rich fractions of the extracts of all samples (i.e. those of 1–2 kDa) were refined by immobilised metal affinity chromatography. The isolated ligands of trace elements were peptides rich in dicarboxylic aminoacids.

Sign in / Sign up

Export Citation Format

Share Document