Design, synthesis, and evaluation of an isotopic labeling strategy for studying fatty acid–protein binding by NMR

2008 ◽  
Vol 4 (6) ◽  
pp. 551 ◽  
Author(s):  
Yong Han ◽  
Timothy E. Alexander ◽  
Gregory P. Tochtrop
Keyword(s):  
Fatty Acid ◽  
Protein Binding ◽  
Isotopic Labeling ◽  
Design Synthesis ◽  
Acid Protein

Exercise attenuates nuclear protein binding to gene regulatory sequences of hepatic fatty acid synthase

1999 ◽  
Vol 87 (3) ◽  
pp. 1009-1015 ◽  
Author(s):  
Russel Fiebig ◽  
Mitchell T. Gore ◽  
Li Li Ji
Keyword(s):  
Fatty Acid ◽  
Protein Binding ◽  
Fatty Acid Synthase ◽  
Nuclear Protein ◽  
Exhaustive Exercise ◽  
Regulatory Sequences ◽  
Transcription Rate ◽  
Response Element ◽  
Hepatic Fatty Acid ◽  
Fas Mrna

The effect of an acute bout of exhaustive exercise on hepatic fatty acid synthase (FAS) gene expression was examined in rats. Female Sprague-Dawley rats (age 8 wk) were fasted for 48 h (F, n = 6), or fasted, refed a high-fructose diet for 6 h, and killed at rest (R, n = 6) or killed after running on a treadmill at 27 m/min and 5% grade for 88 ± 7 min (E, n = 6). Gel mobility shift assay indicated that R rats had twofold higher liver nuclear protein binding to oligonucleotides corresponding to the insulin responsive sequence (−71/−50) and carbohydrate response element (+283/+303) on the FAS promoter, compared with F rats. Exercise severely attenuated this binding in liver nuclear extracts to the levels seen in F rats. Competition and supershift experiments revealed that the bound protein complexes contained the upstream stimulatory factors. Nuclear run-on experiment revealed a 49-fold increase in transcription rate of the FAS gene in R vs. F rats, whereas exercise suppressed the transcription rate. FAS mRNA abundance and FAS enzyme activity were dramatically increased with refeeding but were unaltered by exercise. The results reveal that dietary induction of hepatic FAS is stimulated by increased nuclear protein binding to insulin responsive sequence and carbohydrate response element, whereas exhaustive exercise attenuates the binding, which may precede downregulation of FAS mRNA and enzyme synthesis reported in our previous work (M. A. Griffiths, R. Fiebig, M. T. Gore, D. H. Baker, K. Esser, L. Oscai, and L. L. Ji. J. Nutr. 126, 1959–1971, 1996).

Design, synthesis, and biological evaluation of a series of piperazine ureas as fatty acid amide hydrolase inhibitors

2014 ◽  
Vol 22 (4) ◽  
pp. 1468-1478 ◽  
Author(s):  
Mitsunori Kono ◽  
Takahiro Matsumoto ◽  
Toshihiro Imaeda ◽  
Toru Kawamura ◽  
Shinji Fujimoto ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Amide Hydrolase ◽  
Acid Amide ◽  
Biological Evaluation ◽  
Design Synthesis ◽  
Amide Hydrolase ◽  
Synthesis And Biological Evaluation ◽  
Fatty Acid Amide

Food intake, body mass and gut peptide responses to intrajejunal infusions of a fatty acid, protein or glucose

Appetite ◽  
2009 ◽  
Vol 52 (3) ◽  
pp. 825
Author(s):  
M.J. Dailey ◽  
K.L.K. Tamashiro ◽  
T.H. Moran
Keyword(s):  
Fatty Acid ◽  
Food Intake ◽  
Body Mass ◽  
Acid Protein

The Effect of Renal Insufficiency on Mycophenolic Acid Protein Binding

1999 ◽  
Vol 39 (7) ◽  
pp. 715-720 ◽  
Author(s):  
Bruce Kaplan ◽  
H. U. Meier-Kriesche ◽  
Gary Friedman ◽  
Shamkant Mulgaonkar ◽  
Scott Gruber ◽  
...  
Keyword(s):  
Renal Insufficiency ◽  
Protein Binding ◽  
Mycophenolic Acid ◽  
Acid Protein

Design, synthesis and cytotoxic evaluation of keto-C-glycoside fatty acid conjugates.

1991 ◽  
Vol 1 (12) ◽  
pp. 721-724 ◽  
Author(s):  
Jean Hersovici ◽  
M. Idriss Bennani-Baiti ◽  
Roland Montserret ◽  
Charles Frayssinet ◽  
Kostas Antonakis
Keyword(s):  
Fatty Acid ◽  
Design Synthesis
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