scholarly journals Linear, redox modified DNA probes as electrochemical DNA sensors

2007 ◽  
pp. 3768 ◽  
Author(s):  
Francesco Ricci ◽  
Rebecca Y. Lai ◽  
Kevin W. Plaxco
Keyword(s):  
Dna Probes ◽  
Dna Sensors ◽  
Modified Dna

In situ Hybridization with Hapten-Modified DNA Probes

2015 ◽  
pp. 17-28 ◽  
Author(s):  
A. K. Raap ◽  
R. W. Dirks ◽  
N. M. Jiwa ◽  
P. M. Nederlof ◽  
M. van der Ploeg
Keyword(s):  
In Situ Hybridization ◽  
Dna Probes ◽  
Modified Dna

scholarly journals Establishment and Application of Ligation Reaction-Based Method for Quantifying MicroR-156b

2021 ◽  
Vol 12 ◽  
Author(s):  
Yuxuan He ◽  
Likun Long ◽  
Wei Yan ◽  
Liming Dong ◽  
Wei Xia ◽  
...  
Keyword(s):  
Quantitative Polymerase Chain Reaction ◽  
Dna Probes ◽  
Universal Primers ◽  
Quantitative Detection ◽  
Specific Method ◽  
Biological Regulation ◽  
Ligation Reaction ◽  
Complex Design ◽  
Modified Dna ◽  
Regulation Mechanisms

Microribonucleic acids (miRNAs) play significant roles in the regulation of biological processes and in responses to biotic or abiotic environmental stresses. Therefore, it is necessary to quantitatively detect miRNAs to understand these complicated biological regulation mechanisms. This study established an ultrasensitive and highly specific method for the quantitative detection of miRNAs using simple operations on the ground of the ligation reaction of ribonucleotide-modified deoxyribonucleic acid (DNA) probes. This method avoids the complex design of conventional reverse transcription. In the developed assay, the target miRNA miR156b was able to directly hybridize the two ribonucleotide-modified DNA probes, and amplification with universal primers was achieved following the ligation reaction. As a result, the target miRNA could be sensitively measured even at a detection limit as low as 0.0001 amol, and differences of only a single base could be detected between miR156 family members. Moreover, the proposed quantitative method demonstrated satisfactory results for overexpression-based genetically modified (GM) soybean. Ligation-based quantitative polymerase chain reaction (PCR) therefore has potential in investigating the biological functions of miRNAs, as well as in supervising activities regarding GM products or organisms.

Gold nanoparticle-based 2′-O-methyl modified DNA probes for breast cancerous theranostics

Talanta ◽  
2018 ◽  
Vol 183 ◽  
pp. 11-17 ◽  
Author(s):  
Jing Li ◽  
Jin Huang ◽  
Xiaohai Yang ◽  
Yanjing Yang ◽  
Ke Quan ◽  
...  
Keyword(s):  
Gold Nanoparticle ◽  
Dna Probes ◽  
Modified Dna

Micro Contact Printing of DNA Molecules

2004 ◽  
Author(s):  
H. Zhou ◽  
K. Ma ◽  
G. Jia ◽  
J. Zoval ◽  
M. Madou
Keyword(s):  
Plant Breeding ◽  
Surface Density ◽  
Genetic Disorders ◽  
Rapid Identification ◽  
Dna Probes ◽  
Dna Sensors ◽  
Contact Printing ◽  
Main Challenge ◽  
Human Genetic Disorders ◽  
Probe Layer

The development of DNA sensors has attracted substantial research efforts. Such devices could be used for the rapid identification of pathogens in humans, animals, and plant; in the detection of specific genes in animal and plant breeding; and in the diagnosis of human genetic disorders. The first step to fabricate the DNA sensors is the probe immobilization on the suitable substrate. Traditionally, the DNA probes are spotted on the substrate while the technique hardly controlled the small pattern and surface density of DNA probes. The main challenge here is to achieve probe layer uniformity and the nature of the probe layer itself in few micron and sub-micron feature range.

scholarly journals Chemically Modified DNA Probes That Significantly Improve Mismatch Discrimination

2006 ◽  
Vol 20 (5) ◽  
Author(s):  
Richard Owczarzy ◽  
Yong You ◽  
Bernardo G. Moreira ◽  
Mark A. Behlke
Keyword(s):  
Dna Probes ◽  
Chemically Modified ◽  
Modified Dna ◽  
Mismatch Discrimination

Modulation of RNase H activity by modified DNA probes: Major groove vs minor groove effects

1997 ◽  
Vol 5 (6) ◽  
pp. 1037-1042 ◽  
Author(s):  
Andrew T. Daniher ◽  
Jin Xie ◽  
Shashank Mathur ◽  
James K. Bashkin
Keyword(s):  
Minor Groove ◽  
Dna Probes ◽  
Rnase H ◽  
Major Groove ◽  
Modified Dna

Multiplex ligation-dependent probe amplification (MLPA) for ultrasensitive multiplexed microRNA detection using ribonucleotide-modified DNA probes

2013 ◽  
Vol 49 (85) ◽  
pp. 10013 ◽  
Author(s):  
Pengbo Zhang ◽  
Ye Liu ◽  
Yajing Zhang ◽  
Chenghui Liu ◽  
Zhibin Wang ◽  
...  
Keyword(s):  
Dna Probes ◽  
Microrna Detection ◽  
Modified Dna ◽  
Dependent Probe

Detection of mutations using microarrays of poly(C)10–poly(T)10 modified DNA probes immobilized on agarose films

2006 ◽  
Vol 352 (2) ◽  
pp. 188-197 ◽  
Author(s):  
Martin Dufva ◽  
Jesper Petersen ◽  
Michael Stoltenborg ◽  
Henrik Birgens ◽  
Claus B.V. Christensen
Keyword(s):  
Dna Probes ◽  
Modified Dna ◽  
Detection Of Mutations
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