Characterization of the selenocysteine-containing metabolome in selenium-rich yeast : Part 1. Identification of new species by multi-dimensional liquid chromatography with parallel ICP-MS and electrospray Q-TOFMS/MS detection

2008 ◽  
Vol 23 (1) ◽  
pp. 72-83 ◽  
Author(s):  
Mihaly Dernovics ◽  
Ryszard Lobinski
Keyword(s):  
Liquid Chromatography ◽  
New Species ◽  
Ms Detection ◽  
Icp Ms

Standardless identification of selenocystathionine and its γ-glutamyl derivatives in monkeypot nuts by 3D liquid chromatography with ICP-MS detection followed by nanoHPLC–Q-TOF-MS/MS

The Analyst ◽  
2007 ◽  
Vol 132 (5) ◽  
pp. 439-449 ◽  
Author(s):  
Mihaly Dernovics ◽  
Tamara García-Barrera ◽  
Katarzyna Bierla ◽  
Hugues Preud'homme ◽  
Ryszard Lobinski
Keyword(s):  
Liquid Chromatography ◽  
Ms Detection ◽  
Icp Ms ◽  
Tof Ms

Determination of reduced homocysteine in human serum by elemental labelling and liquid chromatography with ICP-MS and ESI-MS detection

2015 ◽  
Vol 407 (26) ◽  
pp. 7899-7906 ◽  
Author(s):  
Juan Gómez Espina ◽  
Maria Montes-Bayón ◽  
Elisa Blanco-González ◽  
Alfredo Sanz-Medel
Keyword(s):  
Liquid Chromatography ◽  
Human Serum ◽  
Esi Ms ◽  
Ms Detection ◽  
Icp Ms

scholarly journals Searching for Low Molecular Weight Seleno-Compounds in Sprouts by Mass Spectrometry

Molecules ◽  
2020 ◽  
Vol 25 (12) ◽  
pp. 2870 ◽  
Author(s):  
Eliza Kurek ◽  
Magdalena Michalska-Kacymirow ◽  
Anna Konopka ◽  
Olga Kościuczuk ◽  
Anna Tomiak ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Molecular Weight ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Analytical Techniques ◽  
Low Molecular Weight ◽  
Analytical Protocol ◽  
Icp Ms

A fit for purpose analytical protocol was designed towards searching for low molecular weight seleno-compounds in sprouts. Complementary analytical techniques were used to collect information enabling the characterization of selenium speciation. Conceiving the overall characterization of the behavior of selenium, inductively plasma optical mass spectrometry (ICP-MS) was used to determine the total selenium content in entire sprouts as well as in selected extracts or chromatographic fractions. Then, high-performance liquid chromatography combined with ICP-MS (HPLC-ICP-MS) was used to evaluate the presence of inorganic and organic seleno-compounds, with the advantages of being very sensitive towards selenium, but limited by available selenium standard compounds. Finally, ultra-high performance liquid chromatography electrospray ionization triple quadrupole mass spectrometry (UHPLC-ESI-QqQ-MS/MS) and UHPLC-ESI-Orbitrap-MS/MS were used for the confirmation of the identity of selected compounds and identification of several unknown compounds of selenium in vegetable sprouts (sunflower, onion, radish), respectively. Cultivation of plants was designed to supplement sprouts with selenium by using solutions of selenium (IV) at the concentration of 10, 20, 40, and 60 mg/L. The applied methodology allowed to justify that vegetable sprouts metabolize inorganic selenium to a number of organic derivatives, such as seleno-methylselenocysteine (SeMetSeCys), selenomethionine (SeMet), 5′-seleno-adenosine, 2,3-DHP-selenolanthionine, Se-S conjugate of cysteine-selenoglutathione, 2,3-DHP-selenocysteine-cysteine, 2,3-DHP-selenocysteine-cysteinealanine, glutathione-2,3-DHP-selenocysteine, gamma-Glu-MetSeCys or glutamyl-glycinyl-N-2,3-DHP-selenocysteine.

Characterization of metal complexes with metallothioneins by capillary zone electrophoresis (CZE) with ICP-MS and electrospray (ES)-MS detection

2000 ◽  
Vol 15 (6) ◽  
pp. 635-642 ◽  
Author(s):  
Sandra Mounicou ◽  
Kasia Połeć ◽  
Hubert Chassaigne ◽  
Martine Potin-Gautier ◽  
Ryszard Łobiński
Keyword(s):  
Metal Complexes ◽  
Capillary Zone Electrophoresis ◽  
Ms Detection ◽  
Icp Ms ◽  
Zone Electrophoresis ◽  
Capillary Zone
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