Direct electron transfer in nanostructured sol–gel electrodes containing bilirubin oxidase

2007 ◽  
Vol 9 (15) ◽  
pp. 1809-1814 ◽  
Author(s):  
James Lim ◽  
Nicolas Cirigliano ◽  
John Wang ◽  
Bruce Dunn
Keyword(s):  
Electron Transfer ◽  
Direct Electron Transfer ◽  
Sol Gel ◽  
Bilirubin Oxidase

Improved direct electron transfer-type bioelectrocatalysis of bilirubin oxidase using porous gold electrodes

2019 ◽  
Vol 843 ◽  
pp. 47-53 ◽  
Author(s):  
Yui Takahashi ◽  
Mizue Wanibuchi ◽  
Yuki Kitazumi ◽  
Osamu Shirai ◽  
Kenji Kano
Keyword(s):  
Electron Transfer ◽  
Direct Electron Transfer ◽  
Gold Electrodes ◽  
Bilirubin Oxidase ◽  
Porous Gold

scholarly journals Impact of Surface Modification with Gold Nanoparticles on the Bioelectrocatalytic Parameters of Immobilized Bilirubin Oxidase

Acta Naturae ◽  
2014 ◽  
Vol 6 (1) ◽  
pp. 102-106 ◽  
Author(s):  
D. V. Pankratov ◽  
Y. S. Zeifman ◽  
А. V. Dudareva ◽  
G. K. Pankratova ◽  
M. E. Khlupova ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Electron Transfer ◽  
Transfer Rate ◽  
Direct Electron Transfer ◽  
Electron Transfer Rate ◽  
Bilirubin Oxidase ◽  
Heterogeneous Electron Transfer ◽  
Enzyme Molecules ◽  
Different Diameters ◽  
The Impact

We unveil experimental evidence that put into question the widely held notion concerning the impact of nanoparticles on the bioelectrocatalytic parameters of enzymatic electrodes. Comparative studies of the bioelectrocatalytic properties of fungal bilirubin oxidase from Myrothecium verrucaria adsorbed on gold electrodes, modified with gold nanoparticles of different diameters, clearly indicate that neither the direct electron transfer rate (standard heterogeneous electron transfer rate constants were calculated to be 319 s -1) nor the biocatalytic activity of the adsorbed enzyme (bioelectrocatalytic constants were calculated to be 3411 s -1) depends on the size of the nanoparticles, which had diameters close to or larger than those of the enzyme molecules.

scholarly journals Significance of Nano-Structures of Carbon Materials for Direct-Electron-Transfer-type Bioelectrocatalysis of Bilirubin Oxidase

2020 ◽  
Vol 88 (5) ◽  
pp. 374-379 ◽  
Author(s):  
Mizue WANIBUCHI ◽  
Yui TAKAHASHI ◽  
Yuki KITAZUMI ◽  
Osamu SHIRAI ◽  
Kenji KANO
Keyword(s):  
Electron Transfer ◽  
Carbon Materials ◽  
Direct Electron Transfer ◽  
Bilirubin Oxidase ◽  
Nano Structures

Effects of axial ligand mutation of the type I copper site in bilirubin oxidase on direct electron transfer-type bioelectrocatalytic reduction of dioxygen

2007 ◽  
Vol 601 (1-2) ◽  
pp. 119-124 ◽  
Author(s):  
Yuji Kamitaka ◽  
Seiya Tsujimura ◽  
Kunishige Kataoka ◽  
Takeshi Sakurai ◽  
Tokuji Ikeda ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Direct Electron Transfer ◽  
Axial Ligand ◽  
Type I ◽  
Bilirubin Oxidase ◽  
Copper Site

scholarly journals Enhancement of Electrochemical Performance of Bilirubin Oxidase Modified Gas Diffusion Biocathode by Porphyrin Precursor

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Erica Pinchon ◽  
Mary Arugula ◽  
Kapil Pant ◽  
Sameer Singhal
Keyword(s):  
Electron Transfer ◽  
Oxygen Reduction ◽  
Power Density ◽  
Current Density ◽  
Direct Electron Transfer ◽  
Glucose Dehydrogenase ◽  
Phosphate Buffer Solution ◽  
Gas Diffusion ◽  
Biofuel Cell ◽  
Bilirubin Oxidase

Recent studies have focused on tailoring the catalytic currents of multicopper oxidase (MCO) enzymes-based biocathodes to enhance oxygen reduction. Biocathodes modified with natural substrates specific for MCO enzymes demonstrated drastic improvement for oxygen reduction. Performance of 1-pyrenebutanoic acid, succinimidyl ester (PBSE), and 2,5-dimethyl-1-phenyl-1H-pyrrole-3-carbaldehyde (Di-Carb) oriented bilirubin oxidase (BOx) modified gas diffusion biocathode has been highly improved by incorporating hematin, a porphyrin precursor as electron transfer enhancement moiety. Hematin modified electrodes demonstrated direct electron transfer reaction of BOx exhibiting larger O2 reduction in current density in phosphate buffer solution (pH 7.0) without the need of a mediator. A remarkable improvement in the catalytic currents with 2.5-fold increase compared to non-hematin modified oriented BOx electrodes was achieved. Moreover, a mediatorless and compartmentless glucose/O2 biofuel cell based on DET-type bioelectrocatalysis via the BOx cathode and the glucose dehydrogenase (GDH) anode demonstrated peak power densities of 1 mW/cm2 at pH 7.0 with 100 mM glucose/10 mM NAD fuel. The maximum current density of 1.6 mA/cm2 and the maximum power density of 0.4 mW/cm2 were achieved at 300 mV with nonmodified BOx cathode, while 3.5 mA/cm2 and 1.1 mW/cm2 of current and power density were achieved with hematin modified cathode. The performance improved 2.4 times which attributes to the hematin acting as a natural precursor and activator for BOx activity enhancement.

Sign in / Sign up

Export Citation Format

Share Document