Separation distance dependent fluorescence enhancement of fluorescein isothiocyanate by silver nanoparticles

2007 ◽  
pp. 248-250 ◽  
Author(s):  
Daming Cheng ◽  
Qing-Hua Xu
Keyword(s):  
Silver Nanoparticles ◽  
Fluorescence Enhancement ◽  
Fluorescein Isothiocyanate ◽  
Separation Distance

scholarly journals Silver Nanoparticles Functionalized by Fluorescein Isothiocyanate or Rhodamine B Isothiocyanate: Fluorescent and Plasmonic Materials

2021 ◽  
Vol 11 (6) ◽  
pp. 2472
Author(s):  
Ilaria Fratoddi ◽  
Chiara Battocchio ◽  
Giovanna Iucci ◽  
Daniele Catone ◽  
Antonella Cartoni ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Rhodamine B ◽  
Photoelectron Spectroscopy ◽  
Transient Absorption ◽  
Fluorescein Isothiocyanate ◽  
Dye Molecules ◽  
Chemical Surface ◽  
Vis Spectroscopy ◽  
Rhodamine B Isothiocyanate ◽  
Fluorescent Molecules

This paper presents the synthesis of silver nanoparticles (AgNPs) functionalized with fluorescent molecules, in particular with xanthene-based dyes, i.e., fluorescein isothiocyanate (FITC, λmax = 485 nm) and rhodamine B isothiocyanate (RITC, λmax = 555 nm). An in-depth characterization of the particle–dye systems, i.e., AgNPs–RITC and AgNPs–FITC, is presented to evaluate their chemical structure and optical properties due to the interaction between their plasmonic and absorption properties. UV–Vis spectroscopy and the dynamic light scattering (DLS) measurements confirmed the nanosize of the AgNPs–RITC and AgNPs–FITC. Synchrotron radiation X-ray photoelectron spectroscopy (SR-XPS) was used to study the chemical surface functionalization by structural characterization, confirming/examining the isothiocyanate–metal interaction. For AgNPs–RITC, in which the plasmonic and fluorescence peak are not superimposed, the transient dynamics of the dye fluorescence were also studied. Transient absorption measurements showed that by exciting the AgNPs–RITC sample at a wavelength corresponding to the AgNP plasmon resonance, it was possible to preferentially excite the RITC dye molecules attached to the surface of the NPs with respect to the free dye molecules in the solution. These results demonstrate how, by combining plasmonics and fluorescence, these AgNPs can be used as promising systems in biosensing and imaging applications.

Fluorescence Enhancement of Modified Silver Nanoparticles

2011 ◽  
Vol 11 (11) ◽  
pp. 9721-9724 ◽  
Author(s):  
Meicen Liu ◽  
Zhenglong Zhang ◽  
Gaining Liu ◽  
Jun Dong ◽  
Yu Sun ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Fluorescence Enhancement

scholarly journals Cationic Antimicrobial Peptides and Biogenic Silver Nanoparticles Kill Mycobacteria without Eliciting DNA Damage and Cytotoxicity in Mouse Macrophages

2013 ◽  
Vol 57 (8) ◽  
pp. 3688-3698 ◽  
Author(s):  
Soumitra Mohanty ◽  
Prajna Jena ◽  
Ranjit Mehta ◽  
Rashmirekha Pati ◽  
Birendranath Banerjee ◽  
...  
Keyword(s):  
Dna Damage ◽  
Silver Nanoparticles ◽  
Antimicrobial Peptides ◽  
Fluorescein Isothiocyanate ◽  
Antibacterial Activities ◽  
Multidrug Resistant ◽  
Content Type ◽  
Biogenic Silver Nanoparticles ◽  
Transmission Electron ◽  
Mouse Macrophages

ABSTRACTWith the emergence of multidrug-resistant mycobacterial strains, better therapeutic strategies are required for the successful treatment of the infection. Although antimicrobial peptides (AMPs) and silver nanoparticles (AgNPs) are becoming one of the popular antibacterial agents, their antimycobacterial potential is not fully evaluated. In this study, we synthesized biogenic-silver nanoparticles using bacterial, fungal, and plant biomasses and analyzed their antibacterial activities in combination with AMPs against mycobacteria.Mycobacterium smegmatiswas found to be more susceptible to AgNPs compared toM. marinum. We found that NK-2 showed enhanced killing effect with NP-1 and NP-2 biogenic nanoparticles at a 0.5-ppm concentration, whereas LLKKK-18 showed antibacterial activity only with NP-2 at 0.5-ppm dose againstM. smegmatis. In case ofM. marinumNK-2 did not show any additive activity with NP-1 and NP-2 and LLKKK-18 alone completely inhibited the bacterial growth. Both NP-1 and NP-2 also showed increased killing ofM. smegmatisin combination with the antituberculosis drug rifampin. The sizes and shapes of the AgNPs were determined by transmission electron microscopy and dynamic light scattering. AgNPs showed no cytotoxic or DNA damage effects on macrophages at the mycobactericidal dose, whereas treatment with higher doses of AgNPs caused toxicity and micronuclei formation in cytokinesis blocked cells. Macrophages actively endocytosed fluorescein isothiocyanate-labeled AgNPs resulting in nitric oxide independent intracellular killing ofM. smegmatis. Apoptosis and cell cycle studies showed that treatment with higher dose of AgNPs arrested macrophages at the G1-phase. In summary, our data suggest the combined effect of biogenic-AgNPs and antimicrobial peptides as a promising antimycobacterial template.

Integral fluorescence enhancement by silver nanoparticles controlled via PMMA matrix

2011 ◽  
Vol 284 (1) ◽  
pp. 494-497 ◽  
Author(s):  
Yan Jie ◽  
Lu Yonghua ◽  
Wang Pei ◽  
Ming Hai
Keyword(s):  
Silver Nanoparticles ◽  
Fluorescence Enhancement ◽  
Pmma Matrix

Research on Fluorescence Enhancement Effect of Silver Nanoparticles on Zinc Porphyrin

2015 ◽  
Vol 35 (7) ◽  
pp. 0730001
Author(s):  
王静静 Wang Jingjing ◽  
刘莹 Liu Ying ◽  
吴莹 Wu Ying ◽  
孙松 Sun Song
Keyword(s):  
Silver Nanoparticles ◽  
Fluorescence Enhancement ◽  
Enhancement Effect ◽  
Zinc Porphyrin
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