Metabolic monitoring of the electrically stimulated single heart cell within a microfluidic platform

Lab on a Chip ◽  
2006 ◽  
Vol 6 (11) ◽  
pp. 1424 ◽  
Author(s):  
Wei Cheng ◽  
Norbert Klauke ◽  
Helen Sedgwick ◽  
Godfrey L. Smith ◽  
Jonathan M. Cooper
Keyword(s):  
Heart Cell ◽  
Microfluidic Platform ◽  
Metabolic Monitoring ◽  
Single Heart Cell

CHARACTERIZATION OF SINGLE HEART CELL CONTRACTILITY BY RAPID IMAGING

1989 ◽  
Vol 16 (3) ◽  
pp. 179-184 ◽  
Author(s):  
Leanne M. Delbridge ◽  
Peter J. Harris ◽  
Trefor O. Morgan
Keyword(s):  
Heart Cell ◽  
Rapid Imaging ◽  
Cell Contractility ◽  
Single Heart Cell

The bee venom apamin, decreases Isi and increases K+ current in single heart cell

1987 ◽  
Vol 19 ◽  
pp. S41-S41
Author(s):  
G BKAILY ◽  
M BENABDERRAZIK ◽  
Y YAMAMOTO ◽  
D JACQUES ◽  
A SCULPTOREANU ◽  
...  
Keyword(s):  
Bee Venom ◽  
Heart Cell ◽  
K Current ◽  
Single Heart Cell

Bay K 8644 induce enhancement of K+ current in both single heart cell and smooth muscle cell

1989 ◽  
Vol 80 (1-2) ◽  
Author(s):  
J-F Renaud ◽  
G. Bkaily ◽  
M. Benabderrazik ◽  
D. Jacques ◽  
N. Sperelakis
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cell ◽  
Muscle Cell ◽  
Bay K 8644 ◽  
Heart Cell ◽  
K Current ◽  
Single Heart Cell

Ultra-Low-Volume, Real-Time Measurements of Lactate from the Single Heart Cell Using Microsystems Technology

2002 ◽  
Vol 74 (4) ◽  
pp. 908-914 ◽  
Author(s):  
Xinxia Cai ◽  
Norbert Klauke ◽  
Andrew Glidle ◽  
Peter Cobbold ◽  
Godfrey L. Smith ◽  
...  
Keyword(s):  
Real Time ◽  
Heart Cell ◽  
Single Heart Cell ◽  
Low Volume ◽  
Microsystems Technology

Confocal imaging of calcium in intact ventricular muscle provides a new view of excitation-contraction coupling

1996 ◽  
Vol 54 ◽  
pp. 738-739
Author(s):  
W.G. Wier
Keyword(s):  
Local Control ◽  
Cardiac Tissue ◽  
Cell Function ◽  
Isolated Heart ◽  
Cardiac Cells ◽  
Confocal Imaging ◽  
Ion Concentration ◽  
Heart Cell ◽  
Free Calcium ◽  
Heart Cells

A fundamentally new understanding of cardiac excitation-contraction (E-C) coupling is being developed from recent experimental work using confocal microscopy of single isolated heart cells. In particular, the transient change in intracellular free calcium ion concentration ([Ca2+]i transient) that activates muscle contraction is now viewed as resulting from the spatial and temporal summation of small (∼ 8 μm3), subcellular, stereotyped ‘local [Ca2+]i-transients' or, as they have been called, ‘calcium sparks'. This new understanding may be called ‘local control of E-C coupling'. The relevance to normal heart cell function of ‘local control, theory and the recent confocal data on spontaneous Ca2+ ‘sparks', and on electrically evoked local [Ca2+]i-transients has been unknown however, because the previous studies were all conducted on slack, internally perfused, single, enzymatically dissociated cardiac cells, at room temperature, usually with Cs+ replacing K+, and often in the presence of Ca2-channel blockers. The present work was undertaken to establish whether or not the concepts derived from these studies are in fact relevant to normal cardiac tissue under physiological conditions, by attempting to record local [Ca2+]i-transients, sparks (and Ca2+ waves) in intact, multi-cellular cardiac tissue.

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