Wrapping single-walled carbon nanotubes with long single-stranded DNA molecules produced by rolling circle amplification

2006 ◽  
pp. 3582 ◽  
Author(s):  
Weian Zhao ◽  
Yan Gao ◽  
Michael A. Brook ◽  
Yingfu Li
2020 ◽  
Vol MA2020-01 (7) ◽  
pp. 710-710
Author(s):  
Robert Nißler ◽  
Florian Mann ◽  
Parth Chaturvedi ◽  
Jan Horlebein ◽  
Daniel Meyer ◽  
...  

Sensors ◽  
2019 ◽  
Vol 19 (21) ◽  
pp. 4795 ◽  
Author(s):  
Hui Wang ◽  
Pankaj Ramnani ◽  
Tung Pham ◽  
Claudia Chaves Villarreal ◽  
Xuejun Yu ◽  
...  

Volatile organic compounds (VOCs) released by plants are closely associated with plant metabolism and can serve as biomarkers for disease diagnosis. Huanglongbing (HLB), also known as citrus greening or yellow shoot disease, is a lethal threat to the multi-billion-dollar citrus industry. Early detection of HLB is vital for removal of susceptible citrus trees and containment of the disease. Gas sensors are applied to monitor the air quality or toxic gases owing to their low-cost fabrication, smooth operation, and possible miniaturization. Here, we report on the development, characterization, and application of electrical biosensor arrays based on single-walled carbon nanotubes (SWNTs) decorated with single-stranded DNA (ssDNA) for the detection of four VOCs—ethylhexanol, linalool, tetradecene, and phenylacetaldehyde—that serve as secondary biomarkers for detection of infected citrus trees during the asymptomatic stage. SWNTs were noncovalently functionalized with ssDNA using π–π interaction between the nucleotide and sidewall of SWNTs. The resulting ssDNA-SWNT hybrid structure and device properties were investigated using Raman spectroscopy, ultraviolet (UV) spectroscopy, and electrical measurements. To monitor changes in the four VOCs, gas biosensor arrays consisting of bare SWNTs before and after being decorated with different ssDNA were employed to determine the different concentrations of the four VOCs. The data was processed using principal component analysis (PCA) and neural net fitting (NNF).


2016 ◽  
Vol 2016 ◽  
pp. 1-5 ◽  
Author(s):  
Kazuo Umemura ◽  
Katsuki Izumi ◽  
Yoshikazu Kumashiro ◽  
Shusuke Oura ◽  
Teruo Okano

Poly(N-isopropylacrylamide) (PNIPAAm) is one of the most popular thermoresponsive polymers. Adsorption of RecA proteins onto hybrids of PNIPAAm and single-walled carbon nanotubes (SWNTs) was observed in the presence and absence of DNA molecules. Although RecA molecules were adsorbed efficiently onto the hybrid surfaces at 37°C, even in the absence of DNA molecules, the adsorption of RecA was inhibited at 4°C. These results suggest that the thermoresponsive functions of PNIPAAm were effective, even on the SWNT surfaces, which supports the possibility of developing nanobiodevices using PNIPAAm-SWNT hybrids. However, although RecA is a DNA binding protein, there was no significant difference in the adsorption of RecA onto PNIPAAm-SWNT surfaces with and without DNA molecules. This study provides fundamental information for potential biological applications of PNIPAAm-SWNT hybrids.


2014 ◽  
Vol 2 (37) ◽  
pp. 6371-6377 ◽  
Author(s):  
Qingfeng Zhai ◽  
Siqi Zhang ◽  
Hong Jiang ◽  
Qin Wei ◽  
Erkang Wang ◽  
...  

Through SWNTs, duplex DNA derived from folding of single-stranded DNA can be quantitated with Zr4+–PEI coated cone-shaped nanopore. With Hg2+ detection, sensitivity and selectivity based on this paradigm is guaranteed without probe immobilization.


Science ◽  
2009 ◽  
Vol 327 (5961) ◽  
pp. 64-67 ◽  
Author(s):  
H. Liu ◽  
J. He ◽  
J. Tang ◽  
H. Liu ◽  
P. Pang ◽  
...  

Sign in / Sign up

Export Citation Format

Share Document