Efficient protein digestion with peptide separation in a micro-device interfaced to electrospray mass spectrometry

Haider Al-Lawati; Paul Watts; Kevin J. Welham

doi:10.1039/b600752j

Efficient protein digestion with peptide separation in a micro-device interfaced to electrospray mass spectrometry

The Analyst ◽

10.1039/b600752j ◽

2006 ◽

Vol 131 (5) ◽

pp. 656 ◽

Cited By ~ 12

Author(s):

Haider Al-Lawati ◽

Paul Watts ◽

Kevin J. Welham

Keyword(s):

Mass Spectrometry ◽

Electrospray Mass Spectrometry ◽

Protein Digestion ◽

Peptide Separation

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Integration of On-Line Protein Digestion, Peptide Separation, and Protein Identification Using Pepsin-Coated Photopolymerized Sol−Gel Columns and Capillary Electrophoresis/Mass Spectrometry

Analytical Chemistry ◽

10.1021/ac035107u ◽

2004 ◽

Vol 76 (7) ◽

pp. 1896-1902 ◽

Cited By ~ 94

Author(s):

Masaru Kato ◽

Kumiko Sakai-Kato ◽

Jin ◽

Kazuyuki Kubota ◽

Hiroshi Miyano ◽

...

Keyword(s):

Mass Spectrometry ◽

Capillary Electrophoresis ◽

Protein Identification ◽

Sol Gel ◽

Protein Digestion ◽

Peptide Separation ◽

On Line ◽

Capillary Electrophoresis Mass Spectrometry

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Integration of immobilized trypsin bead beds for protein digestion within a microfluidic chip incorporating capillary electrophoresis separations and an electrospray mass spectrometry interface

Rapid Communications in Mass Spectrometry ◽

10.1002/1097-0231(20000815)14:15<1377::aid-rcm31>3.0.co;2-2 ◽

2000 ◽

Vol 14 (15) ◽

pp. 1377-1383 ◽

Cited By ~ 136

Author(s):

Can Wang ◽

Richard Oleschuk ◽

Fahima Ouchen ◽

Jianjun Li ◽

Pierre Thibault ◽

...

Keyword(s):

Mass Spectrometry ◽

Capillary Electrophoresis ◽

Microfluidic Chip ◽

Electrospray Mass Spectrometry ◽

Protein Digestion ◽

Immobilized Trypsin

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Membrane-Based Nanoscale Proteolytic Reactor Enabling Protein Digestion, Peptide Separation, and Protein Identification Using Mass Spectrometry

Analytical Chemistry ◽

10.1021/ac025768b ◽

2003 ◽

Vol 75 (5) ◽

pp. 1067-1074 ◽

Cited By ~ 96

Author(s):

Jonathan W. Cooper ◽

Jinzhi Chen ◽

Yan Li ◽

Cheng S. Lee

Keyword(s):

Mass Spectrometry ◽

Protein Identification ◽

Protein Digestion ◽

Peptide Separation

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An On-Line Protein Digestion Device for Rapid Peptide Mapping by Electrospray Mass Spectrometry

Analytical Chemistry ◽

10.1021/ac000421z ◽

2000 ◽

Vol 72 (21) ◽

pp. 5423-5430 ◽

Cited By ~ 12

Author(s):

Gérald Marie ◽

Laurent Serani ◽

Olivier Laprévote

Keyword(s):

Mass Spectrometry ◽

Electrospray Mass Spectrometry ◽

Peptide Mapping ◽

Protein Digestion ◽

On Line

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Miniaturized membrane-based reversed-phase chromatography and enzyme reactor for protein digestion, peptide separation, and protein identification using electrospray ionization mass spectrometry

Journal of Chromatography A ◽

10.1016/s0021-9673(02)01242-6 ◽

2002 ◽

Vol 979 (1-2) ◽

pp. 241-247 ◽

Cited By ~ 14

Author(s):

Yan Li ◽

Jonathan W Cooper ◽

Cheng S Lee

Keyword(s):

Mass Spectrometry ◽

Electrospray Ionization ◽

Electrospray Ionization Mass Spectrometry ◽

Protein Identification ◽

Reversed Phase ◽

Ionization Mass ◽

Protein Digestion ◽

Enzyme Reactor ◽

Reversed Phase Chromatography ◽

Peptide Separation

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On-line coupling of micro-enzyme reactor with micro-membrane chromatography for protein digestion, peptide separation, and protein identification using electrospray ionization mass spectrometry

Journal of Chromatography A ◽

10.1016/s0021-9673(01)00718-x ◽

2001 ◽

Vol 924 (1-2) ◽

pp. 315-322 ◽

Cited By ~ 38

Author(s):

Yun Jiang ◽

Cheng S Lee

Keyword(s):

Mass Spectrometry ◽

Electrospray Ionization ◽

Electrospray Ionization Mass Spectrometry ◽

Protein Identification ◽

Ionization Mass ◽

Protein Digestion ◽

Enzyme Reactor ◽

Peptide Separation ◽

On Line ◽

Line Coupling

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Faculty Opinions recommendation of Determination of protein-ligand binding constants of a cooperatively regulated tetrameric enzyme using electrospray mass spectrometry.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.718145671.793488431 ◽

2013 ◽

Author(s):

Thomas Kodadek ◽

Xiaodan Liu

Keyword(s):

Mass Spectrometry ◽

Ligand Binding ◽

Electrospray Mass Spectrometry ◽

Binding Constants

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Analysis of Trace Level Perchlorate in Drinking Water and Ground Water by Electrospray Mass Spectrometry

10.21236/ada428122 ◽

1998 ◽

Author(s):

Rebecca A. Clewell ◽

Wayne T. Brashear ◽

David T. Tsui ◽

Sanwat Chaudhuri ◽

Rachel S. Cassady

Keyword(s):

Mass Spectrometry ◽

Drinking Water ◽

Ground Water ◽

Electrospray Mass Spectrometry ◽

Trace Level

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High-performance liquid chromatography–diode array and electrospray-mass spectrometry analysis of non-allowed substances in cosmetic products for preventing hair loss and other hormone-dependent skin diseases

Journal of Pharmaceutical and Biomedical Analysis ◽

10.1016/j.jpba.2008.06.008 ◽

2008 ◽

Vol 48 (3) ◽

pp. 641-648 ◽

Cited By ~ 35

Author(s):

Daniela De Orsi ◽

Manuela Pellegrini ◽

Simona Pichini ◽

Donatella Mattioli ◽

Emilia Marchei ◽

...

Keyword(s):

Mass Spectrometry ◽

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

High Performance ◽

Electrospray Mass Spectrometry ◽

Skin Diseases ◽

Mass Spectrometry Analysis ◽

Diode Array ◽

Cosmetic Products ◽

Spectrometry Analysis

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Determination of synthetic ferric chelates used as fertilizers by liquid chromatography-electrospray/mass spectrometry in agricultural matrices

Journal of the American Society for Mass Spectrometry ◽

10.1016/j.jasms.2006.08.018 ◽

2007 ◽

Vol 18 (1) ◽

pp. 37-47 ◽

Cited By ~ 16

Author(s):

Ana Álvarez-Fernández ◽

Irene Orera ◽

Javier Abadía ◽

Anunciación Abadía

Keyword(s):

Mass Spectrometry ◽

Liquid Chromatography ◽

Electrospray Mass Spectrometry

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