Investigation of matrix effects in boron determination using organic solvents as modifiers for liquid chromatography coupled to ICP-MS

2006 ◽  
Vol 21 (3) ◽  
pp. 305 ◽  
Author(s):  
Niklas Forsgard ◽  
Eva Nilsson ◽  
Marit Andersson ◽  
Jean Pettersson
Keyword(s):  
Liquid Chromatography ◽  
Organic Solvents ◽  
Matrix Effects ◽  
Icp Ms ◽  
Boron Determination

scholarly journals Analytical Methods for Quantification and Identification of Intact Glucosinolates in Arabidopsis Roots Using LC-QqQ(LIT)-MS/MS

Metabolites ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 47
Author(s):  
Kourosh Hooshmand ◽  
Inge S. Fomsgaard
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Crude Extract ◽  
Matrix Effects ◽  
Critical Role ◽  
Reversed Phase ◽  
Biologically Active ◽  
Natural Soil ◽  
Chromatography Method ◽  
Arabidopsis Root

Glucosinolates are biologically active secondary metabolites in Brassicaceae plants that play a critical role in positive and negative interactions between plants and root-associated microbial communities. The aim of this study was to develop a reversed-phase liquid chromatography method to quantify and identify intact glucosinolates in the root of Arabidopsis thaliana (Arabidopsis) grown in non-sterile natural soil by using liquid chromatography-hybrid triple quadruple-linear ion trap (LC-QqQ(LIT)) mass spectrometry. The Synergi Fusion C18-based column was found to be effective for sufficient retention and separation of nine intact glucosinolates without the need for time-consuming desulfation or ion-pairing steps. Method validation results showed satisfactory inter-day and intra-day precision for all glucosinolates except for 4-hydroxyglucobrassicin. Good inter-day and intra-day accuracy and recovery results were observed for glucoiberin, gluconapin, glucobrassicin, 4-methoxyglucobrassicin and neoglucobrassicin. However, for 4-hydroxyglucobrassicin, glucoraphanin and glucoerucin corrections to quantification results might be necessary since the recovery and accuracy results were not optimal. Matrix effects were shown to have a negligible effect on the ionization of all target analytes. The established liquid chromatography–tandem mass spectrometry (LC-MS/MS) method was applied to quantify target intact glucosinolates in Arabidopsis root crude extract of four different wild-type accessions where differences in terms of concentration and composition of intact glucosinolates were observed. Employment of sensitive and selective precursor ion survey scan of m/z 97 in combination with the information-dependent acquisition (IDA) of the enhanced product ion (EPI) dependent scan (Prec97-IDA-EPI) using LC-QqQ(LIT) provided high confidence in structural characterization of diverse intact glucosinolate profiles in complex Arabidopsis root crude extract.

scholarly journals Matrix effects during laser ablation MC ICP-MS analysis of boron isotopes in tourmaline

2014 ◽  
Vol 29 (5) ◽  
pp. 903 ◽  
Author(s):  
Jitka Míková ◽  
Jan Košler ◽  
Michael Wiedenbeck
Keyword(s):  
Laser Ablation ◽  
Matrix Effects ◽  
Boron Isotopes ◽  
Ms Analysis ◽  
Icp Ms

Overcoming Matrix Effects in Liquid Chromatography−Mass Spectrometry

2008 ◽  
Vol 80 (23) ◽  
pp. 9343-9348 ◽  
Author(s):  
Achille Cappiello ◽  
Giorgio Famiglini ◽  
Pierangela Palma ◽  
Elisabetta Pierini ◽  
Veronica Termopoli ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Matrix Effects ◽  
Liquid Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry

Matrix effects using an ICP-MS with a single positive ion lens and grounded stop: analyte mass dependent?

2017 ◽  
Vol 32 (5) ◽  
pp. 951-966 ◽  
Author(s):  
John W. Olesik ◽  
Shi Jiao
Keyword(s):  
Gas Flow ◽  
Matrix Effects ◽  
Mass Matrix ◽  
Gas Flow Rate ◽  
Icp Ms ◽  
Single Positive ◽  
Mass Dependent ◽  
Positive Ion ◽  
Comprehensive Characterization

Comprehensive characterization of ICP-MS matrix effects as a function of analyte mass, matrix mass, lens voltage and nebulizer gas flow rate was carried out.

Some features of the analysis of water, soil and ground in mass spectrometers with inductively coupled plasma (ICP-MS)

2021 ◽  
Vol 5 ◽  
pp. 70-78
Author(s):  
T. K. Nurubeyli ◽  
Keyword(s):  
Matrix Element ◽  
Space Charge ◽  
Inductively Coupled Plasma ◽  
Matrix Effects ◽  
Atomic Emission ◽  
Space Charge Density ◽  
Mass Spectrometers ◽  
Mass Spectral ◽  
Inductively Coupled ◽  
Icp Ms

The paper discusses the possibilities and limitations of the method of mass spectrometry with inductively coupled plasma on the example of elemental analysis of natural and drinking waters, soils and grounds. It is shown that the combination of this method with the simpler atomic emission method makes it possible to expand the range of determined elements, simplify the mass-spectral analysis and increase its reliability. It is shown that the use of the ICP-MS method in the analysis of various objects makes it possible to determine the majority of elements with extremely low detection limits. The reason for the manifestation of matrix effects is the positive space charge formed between the interface and the extractor, the composition of which is determined by the composition of singly charged argon ions. The increase in the concentration of ions in this region is the appearance of a matrix element, which facilitates the scattering of ions from this region. It was found that the heavier the ions of the matrix element, the more the space charge density increases and the scattering occurs. A serious limitation of the method is associated with interferences due to the presence of a certain amount of two and three-charged ions in the plasma. These ions, which have approximately the same mass as the isotopes of the element being determined, are formed as a result of various plasma-chemical reactions and interfere with the determination.

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