Supercritical carbon dioxide foaming of elastomer/heterocyclic methacrylate blends as scaffolds for tissue engineering

2005 ◽  
Vol 15 (46) ◽  
pp. 4881 ◽  
Author(s):  
John J. A. Barry ◽  
Showan N. Nazhat ◽  
Felicity R. A. J. Rose ◽  
Atticus H. Hainsworth ◽  
Colin A. Scotchford ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Tissue Engineering ◽  
Supercritical Carbon Dioxide ◽  
Supercritical Carbon

Putting the fizz into chemistry: applications of supercritical carbon dioxide in tissue engineering, drug delivery and synthesis of novel block copolymers

2007 ◽  
Vol 35 (3) ◽  
pp. 516-521 ◽  
Author(s):  
H. Tai ◽  
V.K. Popov ◽  
K.M. Shakesheff ◽  
S.M. Howdle
Keyword(s):  
Carbon Dioxide ◽  
Drug Delivery ◽  
Tissue Engineering ◽  
Block Copolymers ◽  
Supercritical Carbon Dioxide ◽  
Drug Carriers ◽  
Controlled Polymerization ◽  
Polymeric Scaffolds ◽  
Potential Use ◽  
Supercritical Carbon

This paper describes the recent progress at Nottingham towards the exploitation of the unique properties of scCO2 (supercritical carbon dioxide) for the preparation of polymeric scaffolds for tissue engineering applications and new devices for controlled drug delivery, as well as the synthesis of novel block copolymers by the combination of eROP (enzymatic ring opening polymerization) and controlled polymerization methods for the potential use as drug carriers.

New Designed Decellularized Scaffolds for Scaffold-based Gene Therapy from Elastic Cartilages via Supercritical Carbon Dioxide Fluid and Alkaline/Protease Treatments

2021 ◽  
Vol 21 ◽  
Author(s):  
Ching-Cheng Huang
Keyword(s):  
Carbon Dioxide ◽  
Tissue Engineering ◽  
Gene Therapy ◽  
Supercritical Carbon Dioxide ◽  
Potential Application ◽  
Alkaline Protease ◽  
Thermo Gravimetric Analysis ◽  
Gravimetric Analysis ◽  
Cellular Components ◽  
Supercritical Carbon

Background: Scaffold-based gene therapy provides a promising approach for tissue engineering, which is important and popular, that combines medical applications with engineering materials knowledge. Objective: The decellularization techniques were employed to remove the cellular components from porcine elastic cartilages, leaving a native decellularized extracellular matrix(dECM) composition and architecture integrity of largely insoluble collagen, elastin, and tightly bound glycosaminoglycans. For newly designed collagen scaffold samples, elastic cartilages was hydrolyzed by protease with different concentrations. In this way, it could gain state completely and clearly. Methods: An extraction process of supercritical carbon dioxide(ScCO2) was used to remove cellular components from porcine elastic cartilage. The dECM scaffolds with collagen must be characterized by Fourier transform infrared spectroscopy(FTIR), thermo-gravimetric analysis (TGA), and scanning electron microscope(SEM). Results: The study provided a new treatment combined with supercritical carbon dioxide and alkaline/protease to prepare dECM scaffolds with hole-scaffold microstructures and introduce into a potential application on osteochondral tissue engineering using scaffold-based gene therapy. The new process is simple and efficient. The pore-scaffold microstructures were observed in dECM scaffolds derived from porcine elastic cartilages. The Tdmax values of the resulting dECM scaffolds were observed over 330oC. Conclusion: A series of new scaffolds were successfully obtained from porcine tissue by using ScCO2 and alkaline/enzyme treatments such as an aqueous mixing solution of NH4OH and papain. The dECM scaffolds with high thermal stability were obtained. The resulting scaffold with clean pore-scaffold microstructure could be a potential application for scaffold-based gene therapy.

Scaffold for tissue engineering fabricated by non-isothermal supercritical carbon dioxide foaming of a highly crystalline polyester

2010 ◽  
Vol 6 (1) ◽  
pp. 130-136 ◽  
Author(s):  
Chiara Gualandi ◽  
Lisa J. White ◽  
Liu Chen ◽  
Richard A. Gross ◽  
Kevin M. Shakesheff ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Tissue Engineering ◽  
Supercritical Carbon Dioxide ◽  
Supercritical Carbon
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